A polarized light microscopy method for accurate and reliable grading of collagen organization in cartilage repair

Changoor, Adele; Tran-Khanh, Nicolas; Méthot, Stéphane; Garon, M.; Hurtig, Mark; Shive, Matthew S.; Buschmann, Michael D.

doi:10.1016/j.joca.2010.10.010

Cited by 70 publications

(87 citation statements)

References 62 publications

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“…A quantitative GAG evaluation and collagen typing was not performed in this study. However, examination of our histological sections under a polarised light microscope allowed us to evaluate the loss of GAG content as well as the maintenance or disappearance of the collagen arcades (Módis, 1991;Changoor et al, 2011) in the donor and recipient regions (Figs 5 and 6). In a large multicentre study with human mosaicplasty patients, good gliding surface and histologically proven survival of the transplanted hyaline cartilage was found in 81 of the 98 control arthroscopies (Hangody et al, 2008).…”

Section: Recipient Sitesmentioning

confidence: 99%

Mosaic arthroplasty of the medial femoral condyle in horses — An experimental study

Bodó¹,

Vásárhelyi

Hangody

et al. 2014

Acta Veterinaria Hungarica

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One Arabian and 5 Hungarian half-bred horses were used to study the macroscopic and microscopic survival of autologous osteochondral grafts in the weight-bearing surface of the medial femoral condyle (MFC). Grafts were harvested from the cranial surface of the medial femoral trochlea (MFT) under arthroscopic control. Three of them were transplanted into the weight-bearing surface of the contralateral MFC using an arthrotomy approach. Three months later this transplantation procedure was repeated on the opposite stifle joints in the same animals, but at that time transplantation was performed arthroscopically. Follow-up arthroscopy was carried out 12 months after the first operations, and biopsies were taken from both the recipient and the donor sites for histological examination. During follow-up arthroscopy, the transplanted areas looked congruent and smooth. Microscopically, the characteristics of hyaline cartilage were present in 5 out of the 10 biopsies examined; however, in the other half of biopsies glycosaminoglycan (GAG) loss and change in the architecture of the transplanted cartilage was observed. In a 16-year-old horse, all grafts broke during harvesting, and thus transplantation was not performed. No radiological signs of osteoarthritic changes were detected 9 to 12 months after the operations in the donor and recipient joints. Clinically, no lameness or effusion was present three months after the transplantations.

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Section: Recipient Sitesmentioning

confidence: 99%

Mosaic arthroplasty of the medial femoral condyle in horses — An experimental study

Bodó¹,

Vásárhelyi

Hangody

et al. 2014

Acta Veterinaria Hungarica

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“…Essentially, a polariser filter placed after the light source on a microscope ensures only linearly polarised light is transmitted to the specimen. Optically anisotropic materials change the direction of the polarised light, which is known as birefringence 38 . PLM exploits the natural birefringence of cartilage which varies through the depth of the tissue due to the orientation and the alignment of collagen fibres.…”

Section: Polarised Light Microscopy (Plm)mentioning

confidence: 99%

The role of the superficial region in determining the dynamic properties of articular cartilage

Gannon

Nagel

Kelly

2012

Osteoarthritis and Cartilage

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“…In addition, light microscopy based score systems are used in other areas, such as cartilage research or cytotoxicity assays (Changoor et al, 2011;Grogan et al, 2006). In order to determine the influence of the particulate biomaterials on the ability of PBMCs to develop osteoclast-like cell morphology during culturing, evaluations of cells cultured on glass cover slips, by light microscopy, were performed using a new scoring system.…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

Russmueller

Winkler²,

Lieber³

et al. 2017

eCM

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Much research has been done on bone cells, but only a few studies deal with biomaterial-induced effects on human osteoclasts, which may take on an important role in the successful regeneration of bone. In order to highlight such effects, human peripheral blood mononuclear cells (PBMCs) were extracted from venous blood, differentiated to osteoclasts and then cultured in, the presence of five particulate hydroxyapatite (HA)/β-tricalcium phosphate (TCP) biomaterials, on bovine bone slices and glass cover slips. The biomaterials, AlgOSS 50/50 (50 % HA/50 % TCP), AlgOSS 20/80 (20 % HA/80 % TCP), Algipore (98 % HA), Cerasorb (100 % TCP) and Bio-Oss (100 % HA) were chosen to assess their influence on cell morphology and numbers. Light microscopic evaluation was performed during ongoing cell culture. After 21 d of cultivation, the biomaterial-induced effects on osteoclastic resorption of the bone slices were evaluated by scanning electron microscopy (SEM). Osteoclast-like cells were identified by TRAP staining. All five biomaterials showed larger area fractions of resorbed bone than the control (5.6 ± 6.8 %), as measured on SEM images. The purely hydroxyapatite-based Algipore (9.8 ± 9.7 %) and Bio-Oss (7.9 ± 8.8 %) showed significantly elevated area fraction rates (p ≤ 0.05) of bone resorption. Light Microscope evaluation revealed a significant, but inhibiting effect of Cerasorb (p = 0.05). These data indicated that introducing of small biomaterial hydroxyapatite particles may have improved the performance of bone substitute materials.

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A polarized light microscopy method for accurate and reliable grading of collagen organization in cartilage repair

Abstract: This PLM method offers a novel means for systematically evaluating collagen organization in repair cartilage. We propose that it be used to supplement current gold standard histological scoring systems for a more complete assessment of repair tissue quality.

Cited by 70 publications

References 62 publications

Mosaic arthroplasty of the medial femoral condyle in horses — An experimental study

Mosaic arthroplasty of the medial femoral condyle in horses — An experimental study

The role of the superficial region in determining the dynamic properties of articular cartilage

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

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