A Pollen-Expressed Gene for a Novel Protein with an F-box Motif that is Very Tightly Linked to a Gene for S-RNase in Two Species of Cherry, Prunus cerasus and P. avium

Yamane, Hisayo; Ikeda, Kazuo; Ushijima, Koichiro; Sassa, Hidenori; Tao, Ryutaro

doi:10.1093/pcp/pcg088

Cited by 172 publications

(186 citation statements)

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“…In Rosaceae, the pistil S-determinant is encoded by an S-RNase gene (Bo˘skovic´and Tobutt 1996;Sassa et al 1996;Ushijima et al 1998;Tao et al 1999), whereas, the S-haplotype-specific F-box [SFB]/S-locus F-box [SLF] is a candidate gene for the pollen S-determinant in Prunus species, belonging to the Prunoideae subfamily (Entani et al 2003;Ushijima et al 2003Ushijima et al , 2004Yamane et al 2003a;Zhang et al 2007). However, genes for the pollen S-determinant in Malus species, belonging to the Maloideae subfamily, have not been functionally characterized (Cheng et al 2006;Kakui et al 2007Kakui et al , 2011Okada et al 2008Okada et al , 2011Sassa et al 2007Sassa et al , 2010.…”

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Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Wang

Korban

et al. 2015

Can. J. Plant Sci.

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. 2015. Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species. Can. J. Plant Sci. 95: 213Á225. Most Rosaceae fruit trees such as Prunus and Malus species exhibit gametophytic self-incompatibility that is genetically controlled by the S-locus. In turn, the S-locus contains at least two tightly-linked S-determinant genes, a pistil S-RNase and a pollen SFB. In this study, S-genotypes of 120 cultivated and wild Prunus accessions (peach) and seven wild Malus accessions (crabapple) have been characterized. Among cultivated Prunus genotypes, four S-RNase alleles, designated S 1 , S 2 , S 3 , and S 4 , have been identified, and they share typical structural features of S-RNases from all other self-incompatible Prunus species. Four S-genotypes, S 1 S 2 , S 1 S 3 , S 1 S 4 , and S 2 S 2 , were identified in peach cultivars, while only one S-genotype S 1 S 2 for wild Prunus species. The S 1 S 2 genotype is predominant in peach cultivars, accounting for 58.3% of all evaluated accessions. Similarly, four SFB alleles were identified in peach cultivars and wild accessions. However, all the four SFB alleles encode truncated proteins due to a frame-shift mutation, resulting in loss of hyper-variable and/or variable regions. For Malus species, a total of 14 S-RNase alleles are identified, and of those, two alleles encode truncated proteins. Overall, the genetic variation of both S-RNase and SFB genes in peach is significantly lower than that of S-RNase and SFB genes in self-incompatible Malus and/or Prunus species. The relationship between the genetic variation of SFB genes and the diversification of S-RNase genes in Rosaceae is also discussed.Key words: Prunus, Malus, Self-incompatibility, S-genotypes, S-RNase, Pollen, S-determinant Gu, C., Wang, L., Korban, S. S. et Han, Y. 2015. Identification et caracte´risation des ge`nes de la S-RNase et des ge´notypes-S chez les espe`ces des genres Prunus et Malus. Can. J. Plant Sci. 95: 213Á225. La plupart des arbres fruitiers de la famille des Rosace´es comme ceux des espe`ces des genres Prunus et Malus illustrent une auto-incompatibilite´game´tophytique que controˆle ge´ne´tiquement le locus-S. Ce dernier regroupe au moins deux ge`nes S-de´terminants e´troitement lie´s, un ge`ne S-RNase du pistil et un ge`ne SFB du pollen. Les auteurs ont caracte´rise´le ge´notype-S de 120 obtentions domestique´es et sauvages de peˆcher (Prunus) et de sept obtentions sauvages de pommetier (Malus). Chez les ge´notypes domestique´s de Prunus, ils ont identifie´quatre alle`les du ge`ne S-RNase, baptise´s S 1 , S 2 , S 3 , et S 4 . Ces alle`les partagent les particularite´s structurales communes aux S-RNases des autres espe`ces auto-incompatibles du genre Prunus. Quatre ge´notypes-S, S 1 S 2 , S 1 S 3 , S 1 S 4 , et S 2 S 2 ont aussi e´te´identifie´s chez les cultivars de peˆcher, mais un seul chez les espe`ces sauvages, S 1 S 2 . Le ge´notype S 1 S 2 pre´domine chez les cultivars de peˆcher et repre´sente 58,3 % de toutes les obtentions examine´es. De meˆme, on a ...

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confidence: 99%

Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Wang

Korban

et al. 2015

Can. J. Plant Sci.

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“…Cultivars belonging to the same group cannot be effective pollinators for one another because of gametophytic self-incompatibility (GSI). This character in sweet cherry is attributed to a single multi-allelic gene (S), which controls the synthesis of proteins responsible for the incompatibility (Yamane et al 2003). Fertilisation can occur only when the S-alleles in the haploid genome of the pollen grain differ from the S-alleles of the pistil genome (Wünsch & Hormaza 2004).…”

Section: Introductionmentioning

confidence: 99%

Examination of the Suitability of Different Pollinators for Four Sweet Cherry Cultivars Commonly Grown in Poland

Głowacka

Rozpara

2014

Journal of Horticultural Research

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The aim of the work was to select pollinators for sweet cherry cultivars: 'Vanda', 'Techlovan', 'Sylvia' and 'Regina', which are of great economic significance in Poland. As pollinators, three or four different cultivars with the same or similar flowering time as the pollinated cultivars were evaluated annually. The pollinators belonged to different incompatibility groups. Pollination was carried out over four consecutive seasons. Assuming that for a good yield more than 20% of the flowers should develop fruits, a good pollinator for the cv. 'Vanda' was 'Techlovan', and for the cv. 'Regina' -'Sylvia'. The cv. 'Techlovan' was pollinated to best effect by 'Vega', and the cv. 'Sylvia' by 'Regina', but the setting of fruits in the last two combinations was moderately good. Low to moderately good fruit setting but depending on the year was observed in the combinations 'Sylvia' × 'Summit', 'Regina' × 'Summit', and 'Regina' × 'Rainier'. Cultivar 'Summit' for 'Sylvia', and 'Summit' and 'Rainier' for the cv. 'Regina' should not be used as pollinators because the low percentage of fruit set obtained annually does not guarantee a satisfactory yield.

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“…Among the various SI systems, the predominant form is gametophytic self-incompatibility (GSI), which has been found in 3 plant families, namely Solanaceae, Plantaginaceae, and Rosaceae (McCubbin and Kao, 2000). GSI is controlled by a single multiallelic locus, termed the S locus, which contains at least 2 closely linked genes: 1 pistil determinant S-ribonuclease gene (S-RNase gene) (Ushijima et al, 1998;Tao et al, 1997Tao et al, , 1999 and 1 pollen determinant S-haplotype-specific F-box gene/S locus F-box (SFB/SLF) gene (Entani et al, 2003;Yamane et al, 2003a;Ushijima et al, 2003Ushijima et al, , 2004. Thus, the term haplotype is used to describe variants of the S locus.…”

Section: Introductionmentioning

confidence: 99%

“…After the first identification of S-RNase in the Solanaceae (McClure et al, 1989), a very good candidate gene for the pollen S gene, an SFB, was recently found in several species of Prunus in the Rosaceae, including sweet cherry (P. avium; Yamane et al, 2003a;Ikeda et al, 2004;Sonneveld et al, 2005;Vaughan et al, 2006), almond (P. dulcis; Ushijima et al, 2003Ushijima et al, , 2004Sutherland et al, 2008), Japanese apricot (P. mume; Entani et al, 2003;Yamane et al, 2003b), apricot (P. armeniaca; Romero et al, 2004;Vilanova et al, 2006;Wu et al, 2009), Japanese plum (P. salicina; Zhang et al, 2007), dwarf almond (P. tenella; Šurbanovski et al, 2007), and Chinese cherry (P. pseudocerasus; Huang et al, 2008;Gu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume)

Wang

Gao

Ni³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. SFB, a candidate gene for the pollen S gene, has been identified in several species of Prunus (Rosaceae). We isolated 5 new SFB alleles from 6 Japanese apricot (Prunus mume) lines using a specific Prunus SFB primer pair (SFB-C1F and Pm-Vb), which was designed from conserved regions of Prunus SFB. The nucleotide sequences of these SFB genes were submitted to the GenBank database. The 5 new SFB alleles share typical structural features with SFB alleles from other Prunus species and were found to be polymorphic, with 67.08 to 96.91% amino acid identity. These new SFB alleles were specifically expressed in the pollen. We conclude that the PmSFB alleles that we identified are the pollen S determinants of Japanese apricot; they have potential as a tool for studies of the mechanisms of pollen self-incompatibility.

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A Pollen-Expressed Gene for a Novel Protein with an F-box Motif that is Very Tightly Linked to a Gene for S-RNase in Two Species of Cherry, Prunus cerasus and P. avium

Cited by 172 publications

References 30 publications

Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Examination of the Suitability of Different Pollinators for Four Sweet Cherry Cultivars Commonly Grown in Poland

Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume)

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