A polyion complex sensor array for markerless and noninvasive identification of differentiated mesenchymal stem cells from human adipose tissue

Abstract

A sensor array of cross-reactive polyion complexes enabled markerless and noninvasive identification of osteogenic and adipogenic differentiation of human mesenchymal stem cells.

The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species

“…1). 8,9,17 The catalytic activity of enzymes was reversibly inhibited without denaturing by non-covalent PIC formation with PEGylated polyamines. 18 Since each PIC was expected to possess different affinities for serum proteins, the addition of mammalian sera to PICs would render unique patterns of changes in enzyme activity through competitive interactions between PICs and serum proteins.…”

“…The sensitivity of the PIC sensor array is dependent on the type of enzyme/ substrate pair and the monitoring time for the enzymatic reaction, because the signal of competitive interactions between PICs and analyte proteins are amplified through enzymatic hydrolysis of substrates. We have successfully discriminated sera containing 20 μg/mL proteins, and previously 4.4 to 38.7 μg/mL proteins 8,9 and 0.25 μg/mL secreted proteins from cultured cells, 17 whereas our PIC sensor array would identify more dilute biofluids-based analytes by selecting suited enzyme/ substrate pairs and experimental conditions for target analytes.…”

“…15,16 We found that a cross-reactive PIC sensor array can be used for identifying the source of mammalian sera with 100% accuracy. Our sensing system can recognize proteomic signatures of complex sera, in addition to pure proteins 8,9 and cell culture media containing secreted proteins from human cells, 17 suggesting the versatility of an enzyme-based sensor array to profile real biofluids. The degrees of polymerization of P1 -P3 were 102 for PEG and 35 for PAMA or QPAMA.…”

Aggregative protein–polyelectrolyte complex for high-concentration formulation of protein drugs

“…The salt responsibility of PPC is generally independent of the state of the PPC. The release of protein molecules from a PPC is influenced not only by the incremental increase in ionic strength but also by the addition of other electrolyte, including polyelectrolytes and proteins, and hence are used in several applications, such as purification [36,[41][42][43][44][45], enzyme switch [24,[30][31][32], biosensors [46][47][48], and drug delivery systems [25,49,50]. 6 …”

Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application

“…Seven kinds of serum proteins in the order of 100 nM were identified by a kind of multivariate analysis [60]. This highly discriminative fingerprinting approach can also be used to identify four kinds of homologous mammalian albumins from bovine, equine, human, and rabbit sources [61], 8 mammalian sera [62], and secreted proteins from differentiated mesenchymal stem cells [63].…”