A Polymer/Peptide Complex‐Based Sensor Array That Discriminates Bacteria in Urine

Abstract

An egatively charged poly(para-phenyleneethynylene) (PPE) forms electrostatic complexes with four positively charged antimicrobial peptides (AMP). The AMPs partially quenchthe fluorescence of the PPE and discriminate fourteen different bacteria in water and in human urine by pattern-based fluorescence recognition;t he AMP-PPE complexes bind differentially to the components of bacterial surfaces.T he bacterial species and strains form clusters according to staining properties (Gram-positive and Gram-negative) or genetic similarity (genus,s pecies,a nd strain). The identification and data treatment is performed by pattern evaluation with linear discriminant analysis (LDA) of the collected fluorescence intensity data.

Disposable Paper-Based Biosensors for the Point-of-Care Detection of Hazardous Contaminations—A Review

“…In recent years, with the development of sensor arrays, it has become possible to detect and determine bacteria simultaneously [296][297][298][299][300]. The detection mechanism is based on changes in the optical or electrical properties of the indicator after the interaction with bacteria or metabolites emitted from the bacterial growth.…”

Label‐Free Electrochemiluminescence Aptasensor for Highly Sensitive Detection of Acetylcholinesterase Based on Au‐Nanoparticle‐Functionalized g‐C3N4 Nanohybrid

“…Aptamer is a DNA (deoxyribonucleic acid), RNA (ribonucleic acid) sequence, XNA (nucleic acid analogues) or peptide selected from random nucleic acid molecule libraries via in vitro screening technique named systematic evolution of ligands by an exponential enrichment (SELEX) ,. Aptamers have high specificity and binding affinity to a variety of target molecules such as peptides, drugs, metal ions, proteins, and cells ,,. Besides, owing to good stability, easy labeling, simple synthesis and modification, aptamers can be taken as attractive recognition elements in designing different affinity‐based assays .…”

An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

“…Aptamers possess great promise for the biosensing of macromolecules or low-molecular-weight substrates owing to their good stability, high specicity, cost-effectiveness, and relative ease of isolation and modication. [18][19][20] Various optical, [21][22][23] electrochemical, [24][25][26] and atomic force microscopy (AFM) 27 aptasensors have been developed in the past decade. Among these aptasensors, some require labeling the aptamers using dye molecules for achieving high sensitivity, or functionalizing the aptamers with various functional groups (such as -NH 2 and -SH) to fabricate the sensing interface.…”