A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus

Moraes, Marcia Terezinha Baroni de; Niel, Christian; Gomes, Saint Clair

doi:10.1590/s0100-879x1999000100006

Cited by 21 publications

(19 citation statements)

References 17 publications

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“…These results are in agreement with the studies accomplished by TEDESCHI et al (1989) 38 and CHIARAMONTE et al (1991) 11 although they differ from other studies in which it has been observed higher indexes of detection for HBV-DNA by PCR 2,16,27 and Dot-blot 26 .…”

Section: Discussionsupporting

confidence: 90%

Seroprevalence of hepatitis B virus infection in individuals with clinical evidence of hepatitis in Goiânia, Goiás: detection of viral DNA and determination of subtypes

Silva

Azevedo

Soares

et al. 2002

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe presence of serological markers for hepatitis B virus (HBsAg, anti-HBc IgM and Anti-HBc total) was investigated in the serum of 1,396 individuals who had clinical suspect of hepatitis. It was observed that 50.7% of the individuals were positive and, from the total of the studied individuals, 14.5% were positive for HBsAg. From these, 8.5% were also positive for anti-HBc IgM. The analysis in relation to gender showed a higher seroprevalence index among male individuals (p < 0.0001). It was observed the occurrence of subtypes adw 2 (62.7%), ayw 3 (23.5%), ayw 2 (9.8%) and adw 4 (3.9%). The viral DNA was detected in 61 (33.9%) HBsAg positive samples and in one sample positive only for anti-HBc total. These results indicate an important incidence of the HBV infection in this population, and reinforce previous studies regarding this virus in the central west region of Brazil.

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Section: Discussionsupporting

confidence: 90%

Seroprevalence of hepatitis B virus infection in individuals with clinical evidence of hepatitis in Goiânia, Goiás: detection of viral DNA and determination of subtypes

Silva

Azevedo

Soares

et al. 2002

Rev. Inst. Med. trop. S. Paulo

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“…W hen analyzing samples previously characterized by a monoclonal antibody technique compared with primers for genotype F, we identified genotype A as the most frequent, followed by D and F, which suggested that there was an over-estimation of genotype F in other studies because they used monoclonal antibodies to identify the serotypes 18 .…”

Section: Ethical Considerationsmentioning

confidence: 80%

“…Studies on the distribution of HBV genotypes in Brazil and the Amazon also show that in the northern region, genotype A is the most common, followed by genotype D and genotype F 12,[17][18][19] . However, other studies performed in the Brazilian Amazon revealed that genotype F is more prevalent [20][21][22] .…”

Section: Ethical Considerationsmentioning

confidence: 99%

Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

Dias

Oliveira

Castilho

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Hepatitis B virus (HBV) infection is a serious public health issue worldwide. Hepatitis B virus is classified into eight genotypes, varying from A to H, with distinct geographical distributions. In Brazil, the most frequent genotypes are A, D, and F. METHODS: This study aimed to characterize the HBV genotypes in cases of hepatitis B virus and hepatitis D virus (HDV) co-infections in an endemic area in the Western Brazilian Amazon. We analyzed 86 serum samples reactive for HBsAg from indigenous and non-indigenous populations obtained from previous serological surveys. RESULTS: Of the 86 reactive serum samples, 39 were found to be HBV-DNA-positive by semi-nested PCR. The genotypes were established by sequencing the amplified S gene region. We obtained 20 sequences classified into three genotypes: A, D, and F. Genotype A was the most frequent (60%), followed by D (35%) and F (5%). CONCLUSIONS: The distribution of the HBV genotypes reflected the pattern of historical occupation of the region.

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“…A mixture of one sense primer PS1 (5 0 -CCATATTCTTGGGAACAAGA-3 0 , nt positions 2826-2845) and two antisense primers (S2, 5 0 -GGGTTTAAA-TGTATACCCAAAGA-3 0 , 819-841, and S22, 5 0 -GTATT-TAAATGGATACCCACAGA-3 0 , 819-841) was used. This was because, according to a previous study [Moraes et al, 1999], the simultaneous use of antisense primers S2 and S22, located at the same position on genome, would facilitate the amplification of all HBV genotypes. After an initial DNA denaturation for 3 min at 948C, amplification was for 30 cycles at 958C for 30 sec, 528C for 40 sec, and 728C for 2 min, followed by a final elongation for 7 min at 728C.…”

Section: Hbv Dna Detection and Genotypingmentioning

confidence: 98%

Hepatitis B virus infection in isolated Afro-Brazilian communities

et al. 2005

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The prevalence and genotypes of hepatitis B virus (HBV) have distinct geographical distribution. In Brazil, some African-descendants have been maintained as small isolated communities since the slavery period. In this study, HBV infection among these communities of African origin was examined. Individuals (1,058) living in 12 communities were interviewed and serum samples screened for the presence of HBV markers. HBsAg-positive sera were tested for HBV DNA by PCR and positive samples were genotyped by restriction fragment length polymorphism (RFLP). The overall prevalence of HBV infection was 19.8% (95% CI: 17.5-22.3), ranging from 5.5% to 42.4%, depending on the communities studied. Multivariate analysis of risk factors showed that increasing age, family history of hepatitis, and sexual activity were associated significantly with this infection. HBsAg was detected in 23/1,058 (2.2%) individuals. HBV DNA was present in 2/2 of HBeAg-positive serum samples and in 18/21 (85.7%) anti-HBe-positive samples. All HBV isolates belonged to genotype A, subtype Aa. Three RFLP patterns were identified: AI (17 isolates), AIV (1 isolate), and AVI (2 isolates). These findings suggest a common introduction of HBV during the slave trade from Africa to Brazil.

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A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus

Cited by 21 publications

References 17 publications

Seroprevalence of hepatitis B virus infection in individuals with clinical evidence of hepatitis in Goiânia, Goiás: detection of viral DNA and determination of subtypes

Seroprevalence of hepatitis B virus infection in individuals with clinical evidence of hepatitis in Goiânia, Goiás: detection of viral DNA and determination of subtypes

Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

Hepatitis B virus infection in isolated Afro-Brazilian communities

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