A polymerase chain reaction for the detection of Brucella canis in semen of naturally infected dogs

Keid, Lara Borges; Soares, Rodrigo Martins; Vasconcellos, Sílvio Arruda; Chiebáo, Daniela Pontes; Megid, Jane; Salgado, Vanessa Riesz; Richtzenhain, Leonardo José

doi:10.1016/j.theriogenology.2007.01.003

Cited by 46 publications

(38 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, the sensitivity and/or specificity of PCR for the diagnosis of B. canis might be affected by the type of sample. Studies have demonstrated that in dogs with reproductive problems or naturally infected by B canis, the identification of this pathogen by PCR using vaginal swabs or semen samples is more sensitive comparable to the utilization of PCR derived from blood or serum samples (KEID et al, 2007). Additionally, the utilization of serum samples of infected dogs for the diagnosis of B canis by PCR is of restricted diagnostic value (KEID et al, 2010).…”

mentioning

confidence: 99%

Serological detection of Brucella canis in shelter dogs from Northern Paraná

et al. 2012

View full text Add to dashboard Cite

This study evaluated the presence of circulating antibodies of anti-Brucella canis and anti-B. abortus in shelter dogs maintained in four cities of Northern Paraná. Serum samples of 100 dogs, of both sexes, from the cities of Apucarana, Arapongas, Londrina and Rolândia were collected for the determination of the serological presence of B. canis by the utilization of agarose gel immunodiffusion and B. abortus by the buffered acidified antigen assay. Only 4% (4/100) of the samples evaluated demonstrated seroreactivity to B. canis, with seropositivity varying between 4.17 -10%. However, positive samples originated only from the cities of Apucarana (4.17%; 2/48) and Londrina (10%; 2/20). Seroreactivity to B. abortus antibodies was not verified within the canine population evaluated. These results suggest that B. canis is circulating within the dog population of Northern Paraná. Key words: Canine disease, epidemiology, brucellosis, serology ResumoEste estudo avaliou a presença de anticorpos circulantes anti-Brucella canis e anti-B. abortus em cães mantidos em abrigos situados em quatro municípios da região Norte do Paraná. Amostras séricas de 100 cães, de ambos os sexos, oriundos das cidades de Apucarana, Arapongas, Londrina e Rolândia foram colhidas para determinação sorológica da presença de anticorpos contra B. canis por meio da técnica de imunodifusão em gel de agarose e de anticorpos contra B. abortus por meio da prova do antígeno acidificado tamponado. Somente 4% (4/100) das amostras avaliadas foram sororreagentes a B. canis, com soropositividade variando entre 4.17 -10%. Contudo, as amostras positivas foram oriundas somente das cidades de Apucarana (4.17%; 2/48) e Londrina (10%; 2/20). Não foram verificadas amostras sororreagentes a B. abortus na população canina avaliada. Esses resultados sugerem que B. Canis circula na população de cães da região Norte do Paraná.

show abstract

mentioning

confidence: 99%

Serological detection of Brucella canis in shelter dogs from Northern Paraná

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In dogs, Brucella species DNA has been detected in blood (Keid and others 2007c), semen (Kim and others 2006, Keid and others 2007a) and vaginal swab samples (Keid and others 2007b). Despite the high sensitivity of blood PCR (Keid and others 2007c), the procedure used to extract DNA from whole blood can be time consuming.…”

mentioning

confidence: 99%

Comparison of a PCR assay in whole blood and serum specimens for canine brucellosis diagnosis

et al. 2010

Self Cite

View full text Add to dashboard Cite

The performance of a serum PCR assay was compared with that of a blood PCR assay for the diagnosis of canine brucellosis caused by Brucella canis in 72 dogs. The dogs were classified into three groups (infected, non-infected and suspected brucellosis) according to the results of blood culture and serological tests. The sensitivities of blood PCR and serum PCR were, respectively, 97.14 per cent and 25.71 per cent. The specificities of both were 100 per cent. In the group of dogs with suspected brucellosis, three were positive by blood PCR and none was positive by serum PCR. Serum PCR showed little value for the direct diagnosis of canine brucellosis as the assay had low diagnostic sensitivity and fewer positive dogs were detected by this test than by blood culture, blood PCR, rapid slide agglutination test (RSAT) and RSAT with 2-mercaptoethanol.

show abstract

“…Nowadays, more specific techniques such as Polymerase Chain Reaction (PCR) are used in Brucella spp. detection (Cortez et al, 2001;Manterola et al, 2003;Keid, 2004). The objective of the present study was the adaptation and evaluation of PCR for B. ovis in semen, urine and organs samples collected from experimentally inoculated animals.…”

mentioning

confidence: 99%