A polymorphic microsatellite located within the second intron of the Methylmalonyl-CoA Mutase (MUT) gene on SSC 1

Duscher, S; Peters, Ulrike; Brenig, Bertram

doi:10.1046/j.1365-2052.2000.00664.x

Cited by 3 publications

(5 citation statements)

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“…MUTm was not highly polymorphic in our study population, which had only three alleles of distinct repeat structure and size [allele A: (AT) 5 (AC) 2 , 271 bp; allele B: (AT) 3 ACATAC(AT) 8 , 285 bp and allele C: (AT) 3 ACAT(AC) 2 (AT) 8 , 287 bp]. This finding was unexpected, as no less than 11 alleles have been described for this locus in domestic pigs (Duscher et al. 2000).…”

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 91%

“…2005). The MUT gene () is located in the pig chromosome 1q13–q14, and a (AT) 3 ACATAC(AT) 8 dinucleotide repeat microsatellite <100 bp from the mature peptide in exon 2 () was reported within the second intron (Duscher et al. 1999, 2000), indicating strong linkage of the microsatellite with the gene.…”

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 99%

“…MUTm polymorphisms were analysed using oligonucleotide primers (forward: 5′‐FAM‐TCCTTTCACACCATGTGC‐3′ and reverse: 5′‐AGTACTGTGGAAGAGAGC‐3′) (Duscher et al. 2000).…”

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 99%

“…2004). Although we studied non‐coding microsatellite allele associations with bTB, MUTm is closely linked to a protein‐coding sequence in exon 2 of the MUT gene (Duscher et al. 1999, 2000).…”

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 99%

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Influence of methylmalonyl‐CoA mutase alleles on resistance to bovine tuberculosis in the European wild boar (Sus scrofa)

et al. 2008

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An association study was carried out to examine the influence of methylmalonyl-CoA mutase (MUT) polymorphisms on the susceptibility of a well-studied wild boar population from southern Spain to develop bovine tuberculosis (bTB). To this end, we examined polymorphisms at a closely linked dinucleotide microsatellite flanking exon 2 of the MUT gene in 37 wild boars with bTB and 36 non-infected individuals. The microsatellite showed low polymorphism in the studied population, with only three alleles (MUTm-A, MUTm-B and MUTm-C) found, in contrast to the 11 alleles previously reported for domestic pigs. Our case-control study showed that the MUTm-B allele was associated with disease in a dominant pattern (odds ratio = 3.36; 95% CI = 1.05-10.72; P = 0.04), while the MUTm AA genotype appeared to have a protective effect against bTB infection (odds ratio = 4.33; 95% CI = 1.20-14.96; P = 0.02). Interestingly, infected wild boars heterozygous for MUTm AB are at an advantage (11-fold) to contain the systemic spread of the disease when compared to other genotypes, implying that a balanced polymorphism may be present in the population. These results strengthen previous observations regarding the importance of the MUT gene on bTB resistance in wild boars and indicate that polymorphisms at this locus will influence the risk of acquiring and maintaining bTB in the studied population.

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Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 91%

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 99%

“…MUTm polymorphisms were analysed using oligonucleotide primers (forward: 5′‐FAM‐TCCTTTCACACCATGTGC‐3′ and reverse: 5′‐AGTACTGTGGAAGAGAGC‐3′) (Duscher et al. 2000).…”

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 99%

Section: Distribution Of Mutm Genotypic and Allelic Frequencies Amonmentioning

confidence: 99%

See 2 more Smart Citations

Influence of methylmalonyl‐CoA mutase alleles on resistance to bovine tuberculosis in the European wild boar (Sus scrofa)

et al. 2008

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“…PCR results were analyzed using the interpreting web pages at INRA (http://imprh.toulouse.inra.fr). (Duscher et al, 2000(Duscher et al, , 2003. …”

Section: Methodsmentioning

confidence: 99%

Assignment of the methylmalonyl-CoA mutase gene (MUT) to porcine chromosome 7q13→q14 by in situ hybridization and analysis of radiation hybrid panels

Kierstein

Peters

Habermann

et al. 2003

Cytogenet Genome Res

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Multi-omic network-based interrogation of rat liver metabolism following gastric bypass surgery featuring SWATH proteomics

et al. 2017

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Morbidly obese patients often elect for Roux-en-Y gastric bypass (RYGB), a form of bariatric surgery that triggers a remarkable 30% reduction in excess body weight and reversal of insulin resistance for those who are type II diabetic. A more complete understanding of the underlying molecular mechanisms that drive the complex metabolic reprogramming post-RYGB could lead to innovative non-invasive therapeutics that mimic the beneficial effects of the surgery, namely weight loss, achievement of glycemic control, or reversal of non-alcoholic steatohepatitis (NASH). To facilitate these discoveries, we hereby demonstrate the first multi-omic interrogation of a rodent RYGB model to reveal tissue-specific pathway modules implicated in the control of body weight regulation and energy homeostasis. In this study, we focus on and evaluate liver metabolism three months following RYGB in rats using both SWATH proteomics, a burgeoning label free approach using high resolution mass spectrometry to quantify protein levels in biological samples, as well as MRM metabolomics. The SWATH analysis enabled the quantification of 1378 proteins in liver tissue extracts, of which we report the significant down-regulation of Thrsp and Acot13 in RYGB as putative targets of lipid metabolism for weight loss. Furthermore, we develop a computational graph-based metabolic network module detection algorithm for the discovery of non-canonical pathways, or sub-networks, enriched with significantly elevated or depleted metabolites and proteins in RYGB-treated rat livers. The analysis revealed a network connection between the depleted protein Baat and the depleted metabolite taurine, corroborating the clinical observation that taurine-conjugated bile acid levels are perturbed post-RYGB.

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A polymorphic microsatellite located within the second intron of the Methylmalonyl-CoA Mutase (MUT) gene on SSC 1

Cited by 3 publications

References 4 publications

Influence of methylmalonyl‐CoA mutase alleles on resistance to bovine tuberculosis in the European wild boar (Sus scrofa)

Influence of methylmalonyl‐CoA mutase alleles on resistance to bovine tuberculosis in the European wild boar (Sus scrofa)

Assignment of the methylmalonyl-CoA mutase gene (MUT) to porcine chromosome 7q13→q14 by in situ hybridization and analysis of radiation hybrid panels

Multi-omic network-based interrogation of rat liver metabolism following gastric bypass surgery featuring SWATH proteomics

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