A Polymorphism in the 5′-Untranslated Region of the Porcine Cholecystokinin Type A Receptor Gene Affects Feed Intake and Growth

Houston, Ross D.; Haley, Chris; Archibald, Alan; Cameron, N. D.; Plastow, Graham; Rance, K. A.

doi:10.1534/genetics.106.059659

Cited by 25 publications

(21 citation statements)

References 23 publications

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“…One hypothesis that might explain the observed association between CCKAR genotypes and growth traits is that g. 420 A represses the transcriptional activity of the CCKAR gene, leading to a reduced satiety effect and increased appetite and growth. In pigs, Houston et al (2006) reported that a low-affinity YY1 binding site in the 5′ -UTR of the CCKAR gene might be related to high growth performance. Meanwhile, our data showed that a high affinity YY1 binding site in the 5′ -UTR of the CCKAR gene might be related to high growth performance in chickens.…”

Section: Resultsmentioning

confidence: 99%

Effect of a Single-Nucleotide Polymorphism in the <i>Cholecystokinin Type A Receptor</i> Gene on Growth Traits in the Hinai-dori Chicken Breed

et al. 2013

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We have previously reported the association between chicken cholecystokinin type A receptor gene (CCKAR) haplotypes and growth traits in an F 2 resource population produced by crossing low-and high-growth lines of the Hinai-dori breed. The high-growth line was developed from a low-growth founder from the Preservation Society of the Hinai-dori breed by long-term selection for growth performance at the Akita Prefectural Livestock Experiment Station. In the present study, we determined the effect of a single-nucleotide polymorphism (SNP, AB604331: g.420 C＞A) in the 5′ -untranslated region of CCKAR on the growth traits of the F 2 population. A mismatch amplification mutation polymerase chain reaction assay was developed to distinguish between the 3 genotypes (A/A, A/C, and C/C) in the F 2 population, and the effect of the SNP on growth traits was estimated. The data showed that body weight at 10 and 14 weeks of age, and average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age in individuals with the A allele was superior to that in those with the C allele. The electrophoretic mobility shift assay was performed to clarify the contribution of the g. 420 C＞A SNP in the predicted YY1 binding site. As a result, the YY1 protein showed a stronger binding affinity for g.420 A probe, suggesting the possibility that the SNP affects transcriptional efficiency of the CCKAR. The A allele frequencies in the high-and low-growth lines maintained in fiscal year 2010 were 0.889 and 0.124, respectively. The difference in the allele frequencies of these lines is thought to be caused by long-term selection for growth performance since the probability was too significantly (P＜0.01) low to be caused by random genetic drift.

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Section: Resultsmentioning

confidence: 99%

Effect of a Single-Nucleotide Polymorphism in the <i>Cholecystokinin Type A Receptor</i> Gene on Growth Traits in the Hinai-dori Chicken Breed

et al. 2013

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“…Polymorphisms, including one in a YY1 transcription binding site in the CCKAR promoter, were associated with growth in pigs (28, 29). It was suggested that snp.13.786.12116.S.3 might effect this transcription binding site in chickens, but no evidence was presented (54).…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of the satiety signal receptor CCKAR is responsible for increased growth and body weight during the domestication of chickens

Dunn

Meddle

Wilson

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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Animal domestication has resulted in changes in growth and size. It has been suggested that this may have involved selection for differences in appetite. Divergent growth between chickens selected for egg laying or meat production is one such example. The neurons expressing AGRP and POMC in the basal hypothalamus are important components of appetite regulation, as are the satiety feedback pathways that carry information from the intestine, including CCK and its receptor CCKAR (CCK1 receptor). Using 16 generations of a cross between a fast and a relatively slow growing strain of chicken has identified a region on chromosome 4 downstream of the CCKAR gene, which is responsible for up to a 19% difference in body weight at 12 wk of age. Animals possessing the high-growth haplotype at the locus have lower expression of mRNA and immunoreactive CCKAR in the brain, intestine, and exocrine organs, which is correlated with increased levels of orexigenic AGRP in the hypothalamus. Animals with the high-growth haplotype are resistant to the anorectic effect of exogenously administered CCK, suggesting that their satiety set point has been altered. Comparison with traditional breeds shows that the high-growth haplotype has been present in the founders of modern meat-type strains and may have been selected early in domestication. This is the first dissection of the physiological consequences of a genetic locus for a quantitative trait that alters appetite and gives us an insight into the domestication of animals. This will allow elucidation of how differences in appetite occur in birds and also mammals.

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“…A major focus for the discovery of polymorphisms in production animals has been to identify those associated with traits that are considered to be economically important such as lean muscle accretion, feed efficiency, and growth (Franco et al, 2005;de Oliveira Peixoto et al, 2006;Houston et al, 2006;Verner et al, 2007;Nie et al, 2008). Far fewer studies have identified polymorphisms associated with overall animal health, specifically as it relates to bone health.…”

Section: Discussionmentioning

confidence: 99%

A calcitonin receptor (CALCR) single nucleotide polymorphism is associated with growth performance and bone integrity in response to dietary phosphorus deficiency

Alexander

Cutler

et al. 2010

Journal of Animal Science

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Although concerns over the environmental impact of excess P in the excreta from pig production and governmental regulations have driven research toward reducing dietary supplementation of P to swine diets for over a decade, recent dramatic increases in feed costs have further motivated researchers to identify means to further reduce dietary P supplementation. We have demonstrated that genetic background impacts P utilization in young pigs and have identified genetic polymorphisms in several target genes related to mineral utilization. In this study, we examined the impact of a SNP in the calcitonin receptor gene (CALCR) on P utilization in growing pigs. In Exp. 1, 36 gilts representing the 3 genotypes identified by this CALCR SNP (11, 12, and 22) were fed a P-adequate (PA) or a marginally P-deficient (approximately 20% less available P; PD) diet for 14 wk. As expected, P deficiency reduced plasma P concentration, bone strength, and mineral content (P < 0.05). However, the dietary P deficiency was mild enough to not affect the growth performance of these pigs. A genotype × dietary P interaction (P < 0.05) was observed in measures of bone integrity and mineral content, with the greatest reduction in bone strength and mineral content due to dietary P deficiency being associated with the allele 1. In Exp. 2, 168 pigs from a control line and low residual feed intake (RFI) line were genotyped for the CALCR SNP and fed a PA diet. As expected, pigs from the low RFI line consumed less feed but also gained less BW when compared with the control line (P < 0.05). Although ADFI did not differ between genotypes, pigs having the 11 genotype gained less BW (P < 0.05) than pigs having the 12 or 22 genotypes. Pigs of the 11 and 12 genotypes had bones that tolerated greater load when compared with animals having the 22 genotype (P < 0.05). A similar trend was observed in bone modulus and ash % (P < 0.10). These data are supportive of the association of this CALCR SNP with bone integrity and its response to dietary P restriction. Although the allele 1 is associated with greater bone integrity and mineral content during adequate P nutrition, it is also associated with the greatest loss in bone integrity and mineral content in response to dietary P restriction. Understanding the underlying genetic mechanisms that regulate P utilization may lead to novel strategies to produce more environmentally friendly pigs. ABSTRACT: Although concerns over the environmental impact of excess P in the excreta from pig production and governmental regulations have driven research toward reducing dietary supplementation of P to swine diets for over a decade, recent dramatic increases in feed costs have further motivated researchers to identify means to further reduce dietary P supplementation. We have demonstrated that genetic background impacts P utilization in young pigs and have identified genetic polymorphisms in several target genes related to mineral utilization. In this study, we examined the impact of a SNP in the calcitonin receptor ge...

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A Polymorphism in the 5′-Untranslated Region of the Porcine Cholecystokinin Type A Receptor Gene Affects Feed Intake and Growth

Cited by 25 publications

References 23 publications

Effect of a Single-Nucleotide Polymorphism in the <i>Cholecystokinin Type A Receptor</i> Gene on Growth Traits in the Hinai-dori Chicken Breed

Effect of a Single-Nucleotide Polymorphism in the <i>Cholecystokinin Type A Receptor</i> Gene on Growth Traits in the Hinai-dori Chicken Breed

Decreased expression of the satiety signal receptor CCKAR is responsible for increased growth and body weight during the domestication of chickens

A calcitonin receptor (CALCR) single nucleotide polymorphism is associated with growth performance and bone integrity in response to dietary phosphorus deficiency

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