A poor imitation of a natural process

Zhang, Yemin; Lin, Yao; Yu, Xiuchong; Ou, Jun; Ning, Hui; Liu, Shanrong

doi:10.4161/cc.22575

Cited by 12 publications

(4 citation statements)

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“…93 Nevertheless, the present results strongly suggested that individual somatic cells' ability to enter reprogramming at different time points after transgene induction and the length of time required to complete the reprogramming sequence is greatly impacted by the bioenergetic and anabolic status of the cell, namely the AMPK status (Fig. 6).…”

Section: 53mentioning

confidence: 68%

The mitochondrial H⁺-ATP synthase and the lipogenic switch

et al. 2013

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Section: 53mentioning

confidence: 68%

The mitochondrial H⁺-ATP synthase and the lipogenic switch

et al. 2013

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“…iPS cells generated by this technology are now widely used for understanding many aspects of biology, drug discovery and regenerative medicine [21,65]. However, the efficiency of reprogramming adult tissue cells to ES-like iPS cells remains extremely low, and the full potential of this technology for disease modeling and cell therapy has yet to be realized [18,66]. Although how ML reprograms host cells is a vastly complex process and unknown, certainly the long incubation time within cells gives greater opportunity to alter cellular dynamics without causing oncogenic transformation.…”

Section: Discussionmentioning

confidence: 99%

Bacterial-induced cell reprogramming to stem cell-like cells: new premise in host–pathogen interactions

Hess

Rambukkana

2015

Current Opinion in Microbiology

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Bacterial pathogens employ a myriad of strategies to alter host tissue cell functions for bacterial advantage during infection. Recent advances revealed a fusion of infection biology with stem cell biology by demonstrating developmental reprogramming of lineage committed host glial cells to progenitor/stem cell-like cells by an intracellular bacterial pathogen Mycobacterium leprae. Acquisition of migratory and immunomodulatory properties of such reprogrammed cells provides an added advantage for promoting bacterial spread. This presents a previously unseen sophistication of cell manipulation by hijacking the genomic plasticity of host cells by a human bacterial pathogen. The rationale for such extreme fate conversion of host cells may be directly linked to the exceedingly passive obligate life style of M. leprae with a degraded genome and host cell dependence for both bacterial survival and dissemination, particularly the use of host-derived stem cell-like cells as a vehicle for spreading infection without being detected by immune cells. Thus, this unexpected link between cell reprogramming and infection opens up a new premise in host-pathogen interactions. Furthermore, such bacterial ingenuity could also be harnessed for developing natural ways of reprogramming host cells for repairing damaged tissues from infection, injury and diseases.

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“…However, their populations must be carefully purified to avoid risks of teratoma formation, and chromosomal defects arising over long periods in culture add to tumorogenic risks [6]. Currently, iPSC derivation is relatively inefficient (0.01–1.4%) [7]. The full derivation, expansion, and purification of pluripotent stem cell derived cardiomyocytes can take several months [8, 9].…”

Section: Introductionmentioning

confidence: 99%

Direct reprogramming of mouse fibroblasts to cardiomyocyte-like cells using Yamanaka factors on engineered poly(ethylene glycol) (PEG) hydrogels

Smith¹,

Hoyne

Nguyen

et al. 2013

Biomaterials

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Direct reprogramming strategies enable rapid conversion of somatic cells to cardiomyocytes or cardiomyocyte-like cells without going through the pluripotent state. A recently described protocol couples Yamanaka factor induction with pluripotency inhibition followed by BMP4 treatment to achieve rapid reprogramming of mouse fibroblasts to beating cardiomyocyte-like cells. The original study was performed using Matrigel-coated tissue culture polystyrene (TCPS), a stiff material that also non-specifically adsorbs serum proteins. Protein adsorption-resistant poly(ethylene glycol) (PEG) materials can be covalently modified to present precise concentrations of adhesion proteins or peptides without the unintended effects of non-specifically adsorbed proteins. Here, we describe an improved protocol that incorporates custom-engineered materials. We first reproduced the Efe et al. protocol on Matrigel-coated TCPS (the original material), reprogramming adult mouse tail tip mouse fibroblasts (TTF) and mouse embryonic fibroblasts (MEF) to cardiomyocyte-like cells that demonstrated striated sarcomeric α-actinin staining, spontaneous calcium transients, and visible beating. We then designed poly(ethylene glycol) culture substrates to promote MEF adhesion via laminin and RGD-binding integrins. PEG hydrogels improved proliferation and reprogramming efficiency (evidenced by beating patch number and area, gene expression, and flow cytometry), yielding almost twice the number of sarcomeric α-actinin positive cardiomyocyte-like cells as the originally described substrate. These results illustrate that cellular reprogramming may be enhanced using custom-engineered materials.

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A poor imitation of a natural process

Cited by 12 publications

References 100 publications

The mitochondrial H⁺-ATP synthase and the lipogenic switch

The mitochondrial H⁺-ATP synthase and the lipogenic switch

Bacterial-induced cell reprogramming to stem cell-like cells: new premise in host–pathogen interactions

Direct reprogramming of mouse fibroblasts to cardiomyocyte-like cells using Yamanaka factors on engineered poly(ethylene glycol) (PEG) hydrogels

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A poor imitation of a natural process

Cited by 12 publications

References 100 publications

The mitochondrial H+-ATP synthase and the lipogenic switch

The mitochondrial H+-ATP synthase and the lipogenic switch

Bacterial-induced cell reprogramming to stem cell-like cells: new premise in host–pathogen interactions

Direct reprogramming of mouse fibroblasts to cardiomyocyte-like cells using Yamanaka factors on engineered poly(ethylene glycol) (PEG) hydrogels

Contact Info

Product

Resources

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The mitochondrial H⁺-ATP synthase and the lipogenic switch

The mitochondrial H⁺-ATP synthase and the lipogenic switch