Purpose: Positivity of HBsAg, anti HCV, and anti HIV can expected to be higher in patients treated in an Alcohol and Drug Addiction Treatment Center (ADATC) as they have many risk factors. In this study, screening of these serologic markers in hospitalised patients was aimed. Material and methods: A total of 434 patients with a history of alcohol, injecting or noninjecting drug abuse who received treatment in Bursa ADATC unit in 2016 were included in the study. Patient's files were retrospectively screened for HBsAg, anti HCV, anti HIV positivity and demographic features. Results: Of the 434 patients included in the study with a mean age of 31.95±11.42 years. HbsAg and anti HCV positivity were 2.8%, and 1.4% respectively. None of the patients had HIV positivity. 59.2% of the patients had a family history of drug use. Conclusion: It was found that the prevalence of hepatitis B or hepatitis C was higher in the patients who had begun drug use in 9-12 age range, or in the patients who were homeless, who had no social support and living alone. Screening of these infections in ADATC is important for public health which will provide prevention and treatment of these diseases.