A porcine model of early-onset scoliosis combined with thoracic insufficiency syndrome: Construction and transcriptome analysis

A novel growth-friendly system alleviates pulmonary dysplasia in early-onset scoliosis combined with thoracic insufficiency syndrome: Radiological, pathological, and transcriptomic assessments

“…1 A). As we previously described [ 12 ], the Model and Treatment groups performed the construction of the EOS + TIS model. At 8 weeks, posterior spinal-tethering and rib-tethering devices were removed from piglets in the Model and Treatment groups, and implantation of the novel growth-friendly system was performed in the Treatment group.…”

“…Other operations were consistent with the Model and Treatment groups. As we previously described [ 12 ], X-ray and computed tomography (CT) were performed at the 0th, 8th, 10th, and 12th weeks to assess the spine, thorax, and lungs, coronal (θs) and sagittal Cobb angle (θk), and total lung volume (TLV). Finally, all piglets were humanely euthanized via electric shock, after which lung tissue was removed for subsequent pathological and transcriptomic analysis.…”

“…It is significant to note that we have previously developed a porcine model of the EOS + TIS [ 12 ]. Therefore, the present study intends to comprehensively assess the effects of our system on scoliosis and pulmonary hypoplasia, examining radiological, pathological, and transcriptomic outcomes in this model, while also investigating the underlying molecular mechanisms.…”

Early-onset scoliosis

“…Orthopedics decreased TLV, and pulmonary hypoplasia and described transcriptomic changes in the EOS model that may cause pulmonary hypoplasia [4].…”

“…Simultaneously, the convex hemithorax displays widened intercostal spaces incapable of generating normal expiratory force. Interestingly, a recent study in pig model was able to replicate the common clinical features of EOS such as rib fusion, asymmetric thoracic cage, increased cobb angle, decreased TLV, and pulmonary hypoplasia and described transcriptomic changes in the EOS model that may cause pulmonary hypoplasia [4].…”