A Porcine Model of Familial Adenomatous Polyposis

Flisikowska, Tatiana; Merkl, Claudia; Landmann, Martina; Eser, Stefan; Rezaei, Nousin; Cui, Xinxin; Kurome, Mayuko; Zakhartchenko, Valeri; Keßler, Barbara; Wieland, Hagen; Rottmann, O.; Schmid, Roland M.; Schneider, Günter; Kind, Alexander; Wolf, Eckhard; Saur, Dieter; Schnieke, Angelika

doi:10.1053/j.gastro.2012.07.110

Cited by 116 publications

(111 citation statements)

References 13 publications

Supporting

Mentioning

111

Contrasting

Order By: Relevance

“…It is important to note that a model can also be too large. For example, domestic pig breeds, such as those being used in other cancer models (8)(9)(10), are 2-3 times bigger. This would exclude domestic pigs from most longitudinal monitoring/treatment studies with clinical imaging technologies, as they would quickly outgrow the size capacity of a typical clinical imaging scanner with a bore diameter of 60-70 cm.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development and translational imaging of a TP53 porcine tumorigenesis model

Sieren¹,

Meyerholz²,

Wang³

et al. 2014

J. Clin. Invest.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…Efforts have begun to move cancer-modeling technology previously developed in mice to new species. For example, xenograft models are being tested in immunodeficient pigs (8), and several groups, including our own in the present study, are using gene engineering technology to introduce cancer-related mutations to the porcine genome (9,10).…”

Section: Introductionmentioning

confidence: 99%

Development and translational imaging of a TP53 porcine tumorigenesis model

Sieren¹,

Meyerholz²,

Wang³

et al. 2014

J. Clin. Invest.

View full text Add to dashboard Cite

“…In recent years, improved methods for transgenesis have made this model even more valuable 5 . Humanized pig models include those for retinitis pigmentosa 6 , cystic fibrosis 7 , Alzheimer´s disease 8 , Huntington´s disease 9 , familial adenomatous polyposis 10 , and immunodeficiency 11 . However, transgenesis in the pig, most commonly achieved by pronuclear DNA injection (PNI) or by somatic cell nuclear transfer (SCNT), is an inefficient and expensive process, hampered by poor predictability of levels and patterns of transgene expression 2,3,[12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

et al. 2014

View full text Add to dashboard Cite

2The pig has emerged as an important large animal model in biomedical and pharmaceutical research.We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in pigs by using the Sleeping Beauty transposon system. The protocol is based on co-injection of a plasmid encoding the SB100X hyperactive transposase together with a second plasmid carrying a transgene flanked by binding sites for the transposase, into the cytoplasm of porcine zygotes. The transposase mediates excision of the transgene cassette from the plasmid vector and its permanent insertion into the genome to produce stable transgenic animals. This method compares favorably in terms of both efficiency and reliable transgene expression to classic pronuclear microinjection or somatic cell nuclear transfer, and offers comparable efficacies to lentiviral approaches, without limitations on vector design, issues of transgene silencing as well as the toxicity and biosafety concerns of working with viral vectors. Microinjection of the vectors into zygotes and transfer of the embryos to recipient animals can be performed in one day; generation of germline-transgenic lines by using this protocol takes approximately one year.

show abstract

“…Mesenchymal stem cells, multi-potent tissue stem cells, as well as fibroblasts and kidney cells are already known to support full term development when used as donor cells in pig SCNT [21,23,41,53,[64][65][66][67]. Briefly, mesenchymal stem cells from bone marrow were isolated from femurs and tibias of 6 to 7 months old Landrace x Pietrain pigs [64,68].…”

Section: Generation Of Genetically Modified Pigsmentioning

confidence: 99%

“…Donor cells were isolated by standard methods mainly using collagenase II or trypsin/ EDTA [41]. For details of transfection and characterization of de novo modified cells see references [7,8,41,64]. Cells were used for SCNT at passage 6-8 after additive gene transfer, passage 6-10 after homologous recombination, and passage 2-8 from re-established transgenic pig lines.…”

Section: Generation Of Genetically Modified Pigsmentioning

confidence: 99%

39 Factors Influencing the Efficiency of Generating Genetically Engineered Pigs by Nuclear Transfer: Multi-Factorial Analysis of a Large Data Set

Kurome¹,

Keßler²,

Zakhartchenko³

et al. 2013

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Background: Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells is currently the most widely used strategy to generate tailored pig models for biomedical research. Although this approach facilitates a similar spectrum of genetic modifications as in rodent models, the outcome in terms of live cloned piglets is quite variable. In this study, we aimed at a comprehensive analysis of environmental and experimental factors that are substantially influencing the efficiency of generating genetically engineered pigs. Based on a considerably large data set from 274 SCNT experiments (in total 18,649 reconstructed embryos transferred into 193 recipients), performed over a period of three years, we assessed the relative contribution of season, type of genetic modification, donor cell source, number of cloning rounds, and pre-selection of cloned embryos for early development to the cloning efficiency.

show abstract

A Porcine Model of Familial Adenomatous Polyposis

Cited by 116 publications

References 13 publications

Development and translational imaging of a TP53 porcine tumorigenesis model

Development and translational imaging of a TP53 porcine tumorigenesis model

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

39 Factors Influencing the Efficiency of Generating Genetically Engineered Pigs by Nuclear Transfer: Multi-Factorial Analysis of a Large Data Set

Contact Info

Product

Resources

About