A portable and low cost equipment for flow injection chemiluminescence measurements

Rocha, Fábio R.P.; Ródenas‐Torralba, Eva; Reis, Boaventura F.; Morales‐Rubio, Ángel; Guárdia, Miguel de la

doi:10.1016/j.talanta.2005.03.021

Cited by 72 publications

(39 citation statements)

References 17 publications

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“…19,20 In this work, we intend to develop an automated multicommuted flow procedure for the determination of bromide in L-alanine employing detection by chemiluminescence. The method is based on the bromide oxidation by chloramine-T followed by the reaction of the formed bromine with luminol causing light emission.…”

Section: Indroductionmentioning

confidence: 99%

“…The detection is carried out employing a homemade luminometer described elsewhere. 20,21 In this sense, efforts will be directed to integrate the flow system and the detection unit to obtain a compact and robust equipment. The flow setup will be designed associating an automatic injector and solenoid valves aiming to obtain a versatile analytical module with ability to provided low regent consumption and waste generation.…”

Section: Indroductionmentioning

confidence: 99%

“…The equipment set up comprised a Pentium III microcomputer furnished with an interface card (PCL711S, Advantech Corp) for control and data acquisition; an IPC-8 Ismatec peristaltic pump furnished with Tygon pumping tubes; two three-way solenoid valves (161T031, Nresearch); a homemade automatic injector; 22 a home made luminometer; 20,21 a column machined in acrylic presenting as dimension 15 mm long and 5 mm inner diameter, packed with the anionic resin AG1-X8; reaction coils and flow lines were of polyethylene tubing; and a stabilized 12 V power supplier to feed the solenoid valves. A homemade electronic interface to drive the automatic injector and the solenoid valves based on the integrated circuit ULN2803 that was wired to the output port of the PCL711S interface card by mean of a multiline flat cable.…”

Section: Apparatusmentioning

confidence: 99%

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Automatic flow analysis procedure for the determination of bromide in L-alanine by chemiluminescence detection

Borges¹,

Reis

2007

J. Braz. Chem. Soc.

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Um procedimento automático empregando sistema de análise em fluxo para determinação de brometo em aminoácido L-alanina empregando detecção por quimiluminescência é descrito. O procedimento foi baseado na oxidação do brometo por cloramina-T seguido pela reação com lominol. Fortificando amostras com solução padrão obteve-se recuperação na faixa de 94 a 106 %. Foram obtidas características importantes tais como: resposta linear na faixa de 0,01 até 1,000 mg L ; relative standard deviation of 3.0% (n = 10); sampling throughput of 18 determination per hour; and reagent consumption 0.07 mg luminol and 0.4 mg chloramine-T per determination were achieved.

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Section: Indroductionmentioning

confidence: 99%

Section: Indroductionmentioning

confidence: 99%

Section: Apparatusmentioning

confidence: 99%

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Automatic flow analysis procedure for the determination of bromide in L-alanine by chemiluminescence detection

Borges¹,

Reis

2007

J. Braz. Chem. Soc.

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“…18,19 The use of a photodiode detector and immobilized enzymes in flow systems present some relevant advantages such as simplicity, reliability, low-cost and lower reagent consumption. 18,20 Multicommutation is a powerful alternative to increase the versatility of flow-based procedures, in which discrete commuting devices are employed for solution handling. 21 This approach also presents the advantage of minimizing both reagent consumption and waste generation.…”

Section: Introductionmentioning

confidence: 99%

A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence

Leite¹,

Vieira²,

Fatibello‐Filho³

et al. 2010

J. Braz. Chem. Soc.

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Um sistema de análises em fluxo com multicomutação com detecção por quimiluminescência foi desenvolvido para a determinação de colesterol livre e total sem alterações no módulo de análises. Colesterol estearase e colesterol oxidase foram imobilizadas em esferas de vidro via glutaraldeído/3-aminopropiletoxisilano e mini-colunas contendo as enzimas foram usadas para tratamento das amostras em linha. Ésteres de colesterol foram clivados a colesterol e ácidos graxos na coluna empacotada com colesterol estearase. No reator contendo colesterol oxidase, o analito foi convertido a 4-colesten-3-ona e peróxido de hidrogênio. A detecção foi baseada na quimiluminescência produzida por H 2 O 2 no sistema hexacianoferrato(III)-luminol. Os efeitos de variáveis químicas e hidrodinâmicas sobre o sinal de quimiluminescência foram avaliados. As curvas analíticas foram lineares entre 250 e 2500 mg L -1 e 500 e 4000 mg L -1 , para colesterol livre e total, respectivamente. Limites de detecção para ambos os analitos foram estimados em 60 mg L -1 (99,7% de confiança). A frequência de amostragem foi de 55 h -1 e o consumo de reagentes foi de 350 mg de luminol e 2,6 mg de hexacianoferrato(III) de potássio por determinação. O procedimento desenvolvido foi aplicado à determinação de colesterol em ovos e soro sanguíneo humano gerando resultados concordantes com o procedimento espectrofotométrico de referência com nível de confiança de 95%.A multicommuted flow-based procedure with detection by chemiluminescence for the determination of total and free cholesterol without changes in the flow manifold is proposed. Cholesterol esterase and cholesterol oxidase were both immobilized on glass beads via glutaraldehyde/(3-aminopropyl)-triethoxysilane and mini-columns containing the enzymes were used for online sample treatment. Cholesterol esters were cleaved to cholesterol and fatty acids at the packed reactor containing cholesterol esterase. The reactor containing cholesterol oxidase converted cholesterol to cholest-4-en-3-one also yielding hydrogen peroxide. Detection was based on the chemiluminescence produced by H 2 O 2 in the hexacyanoferrate(III)-luminol system. Influence of both chemical and hydrodynamic variables on the chemiluminescence signals were investigated. The analytical curves were linear from 250 to 2500 mg L -1 and from 500 to 4000 mg L -1 , for free and total cholesterol, respectively. Detection limits for both analytes were estimated as 60 mg L -1 at 99.7% confidence level. The sampling rate was 55 h -1 and reagent consumption was 350 mg of luminol and 2.6 mg of potassium hexacyanoferrate(III) per determination. The procedure developed was successfully applied for determination of cholesterol in eggs and in human blood serum with results in agreement with the reference spectrophotometric method at the 95% confidence level.

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“…The development of a rapid and sensitive method for sensing H2O2 is of great significance for numerous processes. Many analytical methods, such as titrimetry, 2 chemiluminescence, 3 spectrophotometry, 4 and electrochemistry, 5 have been employed for this purpose. Among these, electrochemical detection is one of the promising approaches.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Sensor for Hydrogen Peroxide Based on Immobilized Cytochrome c on Binary Self-assembled Monolayers

Nakamura

2009

ANAL. SCI.

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A portable and low cost equipment for flow injection chemiluminescence measurements

Cited by 72 publications

References 17 publications

Automatic flow analysis procedure for the determination of bromide in L-alanine by chemiluminescence detection

Automatic flow analysis procedure for the determination of bromide in L-alanine by chemiluminescence detection

A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence

Amperometric Sensor for Hydrogen Peroxide Based on Immobilized Cytochrome c on Binary Self-assembled Monolayers

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