A portable culture chamber for studying the effects of hydrostatic pressure on cellular monolayers

Kiyoumarsioskouei, Amir; Saidi, Mohammad R.; Mosadegh, Bobak; Firoozabadi, Bahar

doi:10.1177/0954406218766204

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…The multilayer structure is not observed in subconfluent ECs under pressure 193 , which confirms that it originates from an excessive proliferation of confluent ECs. Physiological pressure further alters endothelial function by increasing intercellular gaps 197 , inducing a reversible loss of monolayer integrity 191,207 , loss of barrier function 200 , and increased permeability 204 . In contrast, low pressure was shown to protect pulmonary endothelial monolayer integrity against inflammatory agents 208 .…”

Section: Flow-derived Mechanical Cuesmentioning

confidence: 99%

Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology

et al. 2021

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Endothelial cells (ECs) lining all blood vessels are subjected to large mechanical stresses that regulate their structure and function in health and disease. Here, we review EC responses to substrate-derived biophysical cues, namely topography, curvature, and stiffness, as well as to flow-derived stresses, notably shear stress, pressure, and tensile stresses. Because these mechanical cues in vivo are coupled and are exerted simultaneously on ECs, we also review the effects of multiple cues and describe burgeoning in vitro approaches for elucidating how ECs integrate and interpret various mechanical stimuli. We conclude by highlighting key open questions and upcoming challenges in the field of EC mechanobiology.

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Section: Flow-derived Mechanical Cuesmentioning

confidence: 99%

Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology

et al. 2021

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“…In addition to flow-induced traction force, hydrostatic pressure from fluid flow may disrupt the vascular endothelialcadherin junction, leading to a multilayer endothelium structure with increased intercellular gap and permeability. [333][334][335] This creates favorable conditions for endothelial cell migration and proliferation, which results in the formation of new vessels.…”

Section: Flow-induced Shear Stressmentioning

confidence: 99%

Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

Shou

Teo

et al. 2023

Advanced Science

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Cells interact with their surrounding environment through a combination of static and dynamic mechanical signals that vary over stimulus types, intensity, space, and time. Compared to static mechanical signals such as stiffness, porosity, and topography, the current understanding on the effects of dynamic mechanical stimulations on cells remains limited, attributing to a lack of access to devices, the complexity of experimental set-up, and data interpretation. Yet, in the pursuit of emerging translational applications (e.g., cell manufacturing for clinical treatment), it is crucial to understand how cells respond to a variety of dynamic forces that are omnipresent in vivo so that they can be exploited to enhance manufacturing and therapeutic outcomes. With a rising appreciation of the extracellular matrix (ECM) as a key regulator of biofunctions, researchers have bioengineered a suite of ECM-mimicking hydrogels, which can be fine-tuned with spatiotemporal mechanical cues to model complex static and dynamic mechanical profiles. This review first discusses how mechanical stimuli may impact different cellular components and the various mechanobiology pathways involved. Then, how hydrogels can be designed to incorporate static and dynamic mechanical parameters to influence cell behaviors are described. The Scopus database is also used to analyze the relative strength in evidence, ranging from strong to weak, based on number of published literatures, associated citations, and treatment significance. Additionally, the impacts of static and dynamic mechanical stimulations on clinically relevant cell types including mesenchymal stem cells, fibroblasts, and immune cells, are evaluated. The aim is to draw attention to the paucity of studies on the effects of dynamic mechanical stimuli on cells, as well as to highlight the potential of using a cocktail of various types and intensities of mechanical stimulations to influence cell fates (similar to the concept of biochemical cocktail to direct cell fate). It is envisioned that this progress report will inspire more exciting translational development of mechanoresponsive hydrogels for biomedical applications.

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A portable culture chamber for studying the effects of hydrostatic pressure on cellular monolayers

Cited by 2 publications

References 40 publications

Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology

Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology

Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

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