A portable kit for rapid detection of bromadiolone in human blood and urine via surface-enhanced raman scattering coupled with salt-induced liquid-liquid phase separation

Shang, Ming; Wei, Haiyan; Gao, Ge; Li, Nianlu; Zou, Wei; Liu, Ranran; Zhang, Mengping; Meng, Xiao; Chen, Wenwen; Sun, Yaxin; Wang, Cuijuan

doi:10.1016/j.snb.2022.132809

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…After adding salt, the particle gaps became smaller and fused, and the dispersed nanoparticles in the colloidal solution agglomerated. Figure D displays that the UV–vis absorption band for the Au@Ag NP solution was detected at 409 nm, which is consistent with a previous study . The observed bandwidth was relatively narrow, further suggesting that the nanoparticles exhibit a high degree of uniformity.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2D displays that the UV−vis absorption band for the Au@Ag NP solution was detected at 409 nm, which is consistent with a previous study. 23 The observed bandwidth was relatively narrow, further suggesting that the nanoparticles exhibit a high degree of uniformity. The uniformity of the NPs provides the basis for efficient SERS enhancement through local surface plasmon resonance.…”

Section: Resultsmentioning

confidence: 99%

“…Preparation of Au@Ag NPs. The synthesis of Au@ Ag nanoparticles (NPs) was conducted by reducing HAuCl 4 or AgNO 3 with sodium citrate, following our established methodology outlined in ref 23. In brief, 100 mL of 0.1% HAuCl 4 aqueous solution was boiled for 15 min at 130 °C.…”

Section: Reagents and Materialsmentioning

confidence: 99%

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Portable SERS-Based POCT Kit for Ultrafast and Sensitive Determining Paraquat in Human Gastric Juice and Urine

Li,

Zhang,

Zhang

et al. 2024

ACS Omega

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Paraquat (PQ) poisoning poses a significant public health concern. Unfortunately, point-of-care testing (POCT) of PQ in biofluids remains challenging. This study developed a portable kit that enables swift and reliable identification and quantification of PQ in human urine and gastric juice. The approach employed the surface-enhanced Raman scattering (SERS) technique, leveraging gold−silver core−shell nanoparticles (Au@Ag NPs) as the substrate. The kit comprised a portable Raman spectrometer and three sealed tubes containing Au@Ag NPs colloid, KI solution, and MgSO 4 solution. A discernible correlation was observed between signal intensity and the logarithmic concentration, spanning from 5 to 500 μg/L in urine and 10 μg/L to 1 mg/L in gastric juice. The detection limits, calculated from the characteristic peak at 1648 cm −1 , were 1.36 and 4.05 μg/L in human urine and gastric juice, respectively. Notably, this POCT kit obviated the need for pretreatment procedures, and the detection process was accomplished within 1 min, yielding satisfactory recoveries. This expeditious time frame is crucial for clinical diagnosis and rescue operations. Compared to conventional methods, this kit demonstrated real-time determinations in nonlaboratory settings. The simplicity and practicality of this POCT assay suggest its significant potential as an innovative alternative for poisoning detection applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Portable SERS-Based POCT Kit for Ultrafast and Sensitive Determining Paraquat in Human Gastric Juice and Urine

Li,

Zhang,

Zhang

et al. 2024

ACS Omega

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“…Previous research indicates that the combination of Raman spectroscopy techniques and machine learning algorithms facilitates the identification and classification of samples for various applications [ 16 , 17 , 18 , 19 ]. The model leverages Raman spectral data’s fingerprint properties and machine learning (ML) algorithms to simplify data processing.…”

Section: Introductionmentioning

confidence: 99%

Performance of Classification Models of Toxins Based on Raman Spectroscopy Using Machine Learning Algorithms

Zhang,

Liu,

et al. 2023

Molecules

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Rapid and accurate detection of protein toxins is crucial for public health. The Raman spectra of several protein toxins, such as abrin, ricin, staphylococcal enterotoxin B (SEB), and bungarotoxin (BGT), have been studied. Multivariate scattering correction (MSC), Savitzky–Golay smoothing (SG), and wavelet transform methods (WT) were applied to preprocess Raman spectra. A principal component analysis (PCA) was used to extract spectral features, and the PCA score plots clustered four toxins with two other proteins. The k-means clustering results show that the spectra processed with MSC and MSC-SG methods have the best classification performance. Then, the two data types were classified using partial least squares discriminant analysis (PLS-DA) with an accuracy of 100%. The prediction results of the PCA and PLS-DA and the partial least squares regression model (PLSR) perform well for the fingerprint region spectra. The PLSR model demonstrates excellent classification and regression ability (accuracy = 100%, Rcv = 0.776). Four toxins were correctly classified with interference from two proteins. Classification models based on spectral feature extraction were established. This strategy shows excellent potential in toxin detection and public health protection. These models provide alternative paths for the development of rapid detection devices.

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New redox behavior and electrochemical sensing of anticoagulant rodenticide bromadiolone using low‐cost graphite electrode: An ultrasensitive tool for forensic and environmental analysis

Conrado,

Pedão,

Ferreira

et al. 2024

Electroanalysis

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A portable kit for rapid detection of bromadiolone in human blood and urine via surface-enhanced raman scattering coupled with salt-induced liquid-liquid phase separation

Cited by 8 publications

References 42 publications

Portable SERS-Based POCT Kit for Ultrafast and Sensitive Determining Paraquat in Human Gastric Juice and Urine

Portable SERS-Based POCT Kit for Ultrafast and Sensitive Determining Paraquat in Human Gastric Juice and Urine

Performance of Classification Models of Toxins Based on Raman Spectroscopy Using Machine Learning Algorithms

New redox behavior and electrochemical sensing of anticoagulant rodenticide bromadiolone using low‐cost graphite electrode: An ultrasensitive tool for forensic and environmental analysis

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