A portable sensor for cell optical density measurement in microfluidic chips

Alam, Muhd Nazrul Hisham Zainal; Kumar, Jayanth Jaya; Whyte, Daniel; Doeven, Egan H.; Kouzani, Abbas Z.

doi:10.1177/0020294018783440

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…The optical components were placed on a probe holder on each end of the miniature bioreactor. A low-cost plastic transmitter (SFH756, Avago Technologies, US) was used to transmit a bright yellow LED light (modulated square wave) from the reference probe at 47 Hz (5v current signal) through the chamber of the miniature bioreactor and is then collected by the receiver photodetector probe (PD) (SFH250, Avago Technologies, US) with similar configuration as the transmitter probe (Zainal Alam et al, 2018). Schematic of the miniature bioreactor platform is illustrated in Figure 1.…”

Section: Design and Fabrication Of The Miniature Bioreactormentioning

confidence: 99%

“…Each sample was loaded into the miniature bioreactor for OD measurement. OD values was attained based on the correlation between the ratio of transmitted and collected analog voltage signals attained (Vs/VT) and different yeast sample concentration (Zainal Alam et al, 2018). A program for automation of the reactor was written in LabVIEW TM Academic Premium Suite software.…”

Section: Process Control and Automation Of The Miniature Bioreactormentioning

confidence: 99%

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Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Arriafdi

Ramachendrin

Alam

2021

MJS

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This paper presents the establishment of a miniature bioreactor platform for anaerobic microbial fermentation processes. It is made from a universal glass bottle and has a working volume of 16 mL. Reactor features included mixing via magnetic stirrer, temperature control via electrical heater and cells optical density (OD) sensing. All sensors and actuators integrated into the reactor were operated using LabVIEW TM (National Instrument, TX, US). The top lid of the bottle was modified to include a 3mm poly(methylmethacrylate) (PMMA) polymer layer where it was machined to provide spaces for fluidic ports and integration of sensors. Each reactor components were sterilized prior to conducting fermentation experiments. Main body of the reactor was made of glass and it was sterilized using the standard heat sterilization method (121 o C for 15 minutes) where else other components were sterilized by exposure to UV light for 2 hours. A series of anaerobic fermentation experiments were conducted in batch mode using S.cerevisiae to evaluate the workability of the system. Fermentation experiments were conducted using inoculum concentration of 2 g•L-1 and starting glucose concentration between 10 g•L-1 and 20 g•L-1. In every experiment, mixing was set to operate at 400 rpm and temperature was adjusted to 30 ± 2 o C. Experiments were carried out until stationary phase was attained. Under these conditions, the best fermentation profile was obtained with glucose concentration of 20 /L where cell specific growth rate was found to be about 0.28 h-1. Bench marking step was also performed where results attained in a miniature bioreactor platform were comparable with the one attained using a 50 mL flask.

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Section: Design and Fabrication Of The Miniature Bioreactormentioning

confidence: 99%

Section: Process Control and Automation Of The Miniature Bioreactormentioning

confidence: 99%

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Arriafdi

Ramachendrin

Alam

2021

MJS

Self Cite

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“…), measurements are usually chosen at around 600 nm [9] . Fungal cultures like yeast can be measured at a wavelength of 660 nm [10] . For the biomass determination of green (C. vulgaris) and blue-green (Phormidium sp.)…”

Section: Introductionmentioning

confidence: 99%

Robust determination of adherent and suspended cancer cell concentration using optical density

Chen

Yu²,

Shi³

et al. 2023

International Conference on Optoelectronic Materials and Devices (ICOMD 2022)

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Optical density is commonly used as a simple and rapid indirect measurement method to estimate biomass concentration in liquid cultures. However, the object of optical density detection is often algae cells, colonies, and other microorganisms， few studies adopt optical density to quantitatively measure the concentration of cancer cells. In this paper, different liquid media and cancer cells were used to implement a full-wavelength spectral analysis by microplate reader to find the corresponding robust wavelength. According to the experimental results, we suggest measuring cell concentration at nearinfrared wavelengths, such as 850 nm, to facilitate the subsequent unification of protocols applicable to different cell types and culture conditions. Meanwhile, a portable flow cell sensor based on optical density is demonstrated. The calibration curves under various experimental conditions have high regression coefficient R 2 values, all greater than 0.99, which are expected to be used for online real-time measurement of cell concentration in biological reaction processes. This study provides the feasibility of using the optical density method as a quick and easy way for indirect quantitative measurement of cancer cell concentration.

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“…Some of the light is scattered where else some passed through the suspended cells and read by a photo detector, which outputs a value. The concentration of the suspended cells and the transmitted light can indeed be correlated by the application of the Beer-Lambert's law (Laganovska et al, 2022;Zainal Alam et al, 2018). Often a linear relation is obtained over a certain range of cells concentration and this correlation is used to estimate the cells concentration (cells OD) within the measured sample.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Portable Arduino-based Mini Spectrometer Prototype for Cells Optical Density Measurement

Zainal Alam

2023

BBT

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Cell optical density (OD) measurement is essential for tracking the progress of microbial fermentation processes. It is typically performed through a spectrophotometer at the wavelength of 600 nm. In this paper, a portable Arduino-based mini spectrometer prototype is presented. In the proposed prototype, a turbidity sensor was utilized to perform the optical density measurement of cells based on the Beer’s Lambert law. Differences on the light intensity from the excitation source and the collective point were correlated with the known cell concentration (using S. cerevisae yeast with concentration between 1 g/L and 5 g/L). The data attained was used as the standard curve and also to validate the functionality of the device. Fermentation experiments were carried out and the progress of the fermentation were measured using the proposed OD sensing prototype and compared to the standard bench top spectrometer. Both devices produced a calibration curve with R-squared value greater than 0.95 (0.95 for bench spectrometer and 0.97 for the Arduino device) and a comparable fermentation curve attained from both devices highlighted the workability of the proposed prototype. In addition, the device has a small footprint, portable and affordable.

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A portable sensor for cell optical density measurement in microfluidic chips

Cited by 7 publications

References 22 publications

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Robust determination of adherent and suspended cancer cell concentration using optical density

Evaluation of a Portable Arduino-based Mini Spectrometer Prototype for Cells Optical Density Measurement

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