1992
DOI: 10.1016/0378-1119(92)90217-d
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A position- and orientation-dependent element in the first intron is required for expression of the mouse hprt gene in embryonic stem cells

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Cited by 21 publications
(9 citation statements)
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“…Placing the UbC intron, alone or with the flanking exon 1, upstream of the proximal promoter, in both sense and antisense orientation, caused a drastic drop of luciferase expression, demonstrating that the intron was not able to support expression when localized outside the transcribed region, even if the other promoter elements were present. This evidence suggests that the UbC intron is not acting as a typical transcriptional enhancer, but rather regulates gene expression in a context-dependent manner, as reported for other intronic cis -elements which affect promoter activity orientation- or orientation- and position-dependently [45], [46].…”
Section: Discussionsupporting
confidence: 58%
“…Placing the UbC intron, alone or with the flanking exon 1, upstream of the proximal promoter, in both sense and antisense orientation, caused a drastic drop of luciferase expression, demonstrating that the intron was not able to support expression when localized outside the transcribed region, even if the other promoter elements were present. This evidence suggests that the UbC intron is not acting as a typical transcriptional enhancer, but rather regulates gene expression in a context-dependent manner, as reported for other intronic cis -elements which affect promoter activity orientation- or orientation- and position-dependently [45], [46].…”
Section: Discussionsupporting
confidence: 58%
“…Because the predominant retarded band of gel‐mobility shift experiments was obtained using the intronic probe P2 containing one putative octamer‐binding site and EcRE half‐site, the short intron 1, characteristic of cuticular protein genes, could be considered as an important element in the transcriptional regulation of ACP‐20 . Increasing numbers of studies emphasize the importance of regulatory elements located within introns, and most often in intron 1, of housekeeping as well as tissue‐specifically expressed genes, and such sequences are characterized as positive or negative regulatory elements, enhancers or silencers and as alternative intronic promoters (reviewed in Magin et al ., 1992; Fatyol et al ., 1999; Wang et al ., 2002; Kolb, 2003). It has also been reported that the mere presence of an intron can increase gene expression levels and that an exogenous intron could be recognized by the splicing machinery, leading to an increase in transgene expression levels (Palmiter et al ., 1991; Choi et al ., 1991; Duncker et al ., 1997).…”
Section: Discussionmentioning
confidence: 99%
“…Classically, cis-enhanding elements have been reported to up-regulate the homologous promoter in an orientation-and positionindependent manner. However, recently, some intronic enhancers have been reported to up-regulate the homologous and/or heterologous promoter orientationdependently [41,42], or orientation-and positiondependently [43]. We assume that the CAGCTG motif in intron 1 also regulates the gene expression in a contextdependent manner.…”
Section: Discussionmentioning
confidence: 99%