A positive transcription factor in osmotic stress tolerance, ZAT10, is regulated by MAP kinases in Arabidopsis

Nguyen, Xuan Canh; Kim, Sun Ho; Hussain, Shah; An, Jonguk; Yoo, Yeji; Han, Hay Ju; Yoo, Ju Soon; Lim, Chae Oh; Yun, Dae‐Jin; Chung, Woo Sik

doi:10.1007/s12374-016-0442-4

Cited by 27 publications

(20 citation statements)

References 31 publications

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“…ZAT10 is phosphorylated by MPKs that are thought to function in abiotic stress tolerance (Nguyen et al, 2012). ZAT10 is a positive regulator of osmotic stress responses that is regulated by MAP kinases in Arabidopsis (Nguyen et al, 2016). In rice, RZF71 was strongly induced by 20% PEG6000 treatment, suggesting that RZF71 plays an important role in the response to osmotic stress (Guo et al, 2007).…”

Section: Roles In Osmotic Stressmentioning

confidence: 99%

C2H2 Zinc Finger Proteins: Master Regulators of Abiotic Stress Responses in Plants

et al. 2020

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Abiotic stresses such as drought and salinity are major environmental factors that limit crop yields. Unraveling the molecular mechanisms underlying abiotic stress resistance is crucial for improving crop performance and increasing productivity under adverse environmental conditions. Zinc finger proteins, comprising one of the largest transcription factor families, are known for their finger-like structure and their ability to bind Zn 2+ . Zinc finger proteins are categorized into nine subfamilies based on their conserved Cys and His motifs, including the Cys2/His2-type (C2H2), C3H, C3HC4, C2HC5, C4HC3, C2HC, C4, C6, and C8 subfamilies. Over the past two decades, much progress has been made in understanding the roles of C2H2 zinc finger proteins in plant growth, development, and stress signal transduction. In this review, we focus on recent progress in elucidating the structures, functions, and classifications of plant C2H2 zinc finger proteins and their roles in abiotic stress responses. FIGURE 1 | Structure of C2H2 zinc finger proteins. Structural model of the Arabidopsis C2H2 zinc finger protein STZ produced using the Protein Model Portal tool. Han et al.

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Section: Roles In Osmotic Stressmentioning

confidence: 99%

C2H2 Zinc Finger Proteins: Master Regulators of Abiotic Stress Responses in Plants

et al. 2020

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“…Arabidopsis plants overexpressing ZAT10 exhibited growth retardation and enhanced tolerance to drought, salt, osmotic, heat, and oxidative stress, as well as photo-inhibitory light [ 62 – 64 ]. Although Mittler and coworkers [ 62 ] observed that knockout and RNAi mutants of Zat10 were more tolerant to osmotic and salinity stress, it has recently been reported that this gene functions as a positive regulator in osmotic stress tolerance, withZAT10 phosphorylation being required for its function in Arabidopsis [ 65 ]. We propose that the transcription factors identified in our study are promising candidate genes for controlling chilling tolerance in zucchini, especially those specifically induced by low temperature in the cold-tolerant cultivar Natura (MYB76-like, CUUC97743; AP2/ERF-like, CUUC104934; and ZAT10-like, CUUC61049).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity

et al. 2018

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BackgroundZucchini fruit is susceptible to chilling injury (CI), but the response to low storage temperature is cultivar dependent. Previous reports about the response of zucchini fruit to chilling storage have been focused on the physiology and biochemistry of this process, with little information about the molecular mechanisms underlying it. In this work, we present a comprehensive analysis of transcriptomic changes that take place after cold storage in zucchini fruit of two commercial cultivars with contrasting response to chilling stress.ResultsRNA-Seq analysis was conducted in exocarp of fruit at harvest and after 14 days of storage at 4 and 20 °C. Differential expressed genes (DEGs) were obtained comparing fruit stored at 4 °C with their control at 20 °C, and then specific and common up and down-regulated DEGs of each cultivar were identified. Functional analysis of these DEGs identified similarities between the response of zucchini fruit to low temperature and other stresses, with an important number of GO terms related to biotic and abiotic stresses overrepresented in both cultivars. This study also revealed several molecular mechanisms that could be related to chilling tolerance, since they were up-regulated in cv. Natura (CI tolerant) or down-regulated in cv. Sinatra (CI sensitive). These mechanisms were mainly those related to carbohydrate and energy metabolism, transcription, signal transduction, and protein transport and degradation. Among DEGs belonging to these pathways, we selected candidate genes that could regulate or promote chilling tolerance in zucchini fruit including the transcription factors MYB76-like, ZAT10-like, DELLA protein GAIP, and AP2/ERF domain-containing protein.ConclusionsThis study provides a broader understanding of the important mechanisms and processes related to coping with low temperature stress in zucchini fruit and allowed the identification of some candidate genes that may be involved in the acquisition of chilling tolerance in this crop. These genes will be the basis of future studies aimed to identify markers involved in cold tolerance and aid in zucchini breeding programs.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4500-9) contains supplementary material, which is available to authorized users.

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“…The C2H2‐type zinc finger, also named the TRANSCRIPTION FACTOR IIIA (TFIIIA)‐type finger, was first identified in Xenopus sp. and represents an important class of eukaryotic TFs (Nguyen et al ., ). The C2H2‐type zinc finger domain includes the ‘CX2‐4CX3FX5LX2HX3‐5H’ motif, which is unique to plant zinc finger proteins, and consists of about two pairs of conserved cysteine and histidine tetrahedrally bound to a zinc ion and 30 amino acids (Pabo et al ., ; Takatsuji, ).…”

Section: Introductionmentioning

confidence: 97%

PeSTZ1, a C2H2‐type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2

Wang

et al. 2019

Plant Biotechnology Journal

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Summary In the present study, PeSTZ1, a cysteine‐2/histidine‐2‐type zinc finger transcription factor, was isolated from the desert poplar, Populus euphratica, which serves as a model stress adaptation system for trees. PeSTZ1 was preferentially expressed in the young stems and was significantly up‐regulated during chilling and freezing treatments. PeSTZ1 was localized to the nucleus and bound specifically to the PeAPX2 promoter. To examine the potential functions of PeSTZ1, we overexpressed it in poplar 84K hybrids (Populus alba × Populus glandulosa), which are known to be stress‐sensitive. Upon exposure to freezing stress, transgenic poplars maintained higher photosynthetic activity and dissipated more excess light energy (in the form of heat) than wild‐type poplars. Thus, PeSTZ1 functions as a transcription activator to enhance freezing tolerance without sacrificing growth. Under freezing stress, PeSTZ1 acts upstream of ASCORBATE PEROXIDASE2 (PeAPX2) and directly regulates its expression by binding to its promoter. Activated PeAPX2 promotes cytosolic APX that scavenges reactive oxygen species (ROS) under cold stress. PeSTZ1 may operate in parallel with C‐REPEAT‐BINDING FACTORS to regulate COLD‐REGULATED gene expression. Moreover, PeSTZ1 up‐regulation reduces malondialdehyde and ROS accumulation by activating the antioxidant system. Taken together, these results suggested that overexpressing PeSTZ1 in 84K poplar enhances freezing tolerance through the modulation of ROS scavenging via the direct regulation of PeAPX2 expression.

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A positive transcription factor in osmotic stress tolerance, ZAT10, is regulated by MAP kinases in Arabidopsis

Cited by 27 publications

References 31 publications

C2H2 Zinc Finger Proteins: Master Regulators of Abiotic Stress Responses in Plants

C2H2 Zinc Finger Proteins: Master Regulators of Abiotic Stress Responses in Plants

Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity

PeSTZ1, a C2H2‐type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2

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