A possibility of chitinolytic bacteria utilization to control basal stems disease caused by Ganoderma boninense in oil palm seedling

Abstract

The utilization of chitinolytic bacterial isolates Enterobacter sp. KR05, Enterobacter cloacae LK08, Bacillus sp. BK13, Enterobacter sp. BK15, and Bacillus sp. BK17 to control basal stem rot disease caused by Ganoderma boninense in oil palm seedling was studied. Antagonistic assay of chitinolytic bacterial isolates to G. boninense was conducted in minimum salt medium agar with 2% colloidal chitin as sole carbon source. To examine ability of the isolates in reducing basal stem rot disease incidence, the 3 to 4 months old of oil palm seedlings were treated by pouring oil palm seedling growing media with chitinolytic bacterial isolates a day prior infestation of G. boninense spores. The result showed that all chitinolytic isolates inhibited the growth of G. boninense in vitro. Hyphal abnormalities that is, dwarf, tiny, curled, twisted and bulby hyphae were observed after antagonistic assay. All chitinolytic isolates were able to reduce the disease incidence on the oil palm seedling to some extent. The isolates might infest into the oil palm seedling root as endophytes.

Potential of Chitinolytic Bacillus amyloliquefaciens SAHA 12.07 and Serratia marcescens KAHN 15.12 as Biocontrol Agents of Ganoderma boninense

“…BK17 were also able to suppressed the growth of G. boninense in chitin media agar (Suryanto et al, 2012) but in our study using PDA as a good media for the growth of fungi. The result was indicated by the growth of G. boninense on chitin media during antagonistic test against bacteria which has lower fungal mycelium density (Suryanto et al, 2012), compared to the growth of G. boninense on PDA media (control) in this study. The efforts to get a potential biocontrol agent during antagonist test is needed to maintain all the conditions of well growth the pathogen.…”

“…The control effort of BSR disease using technical culture and chemical fungicides still does not show the maximum results, even causing problems such as environmental pollution and resistance of pathogen to fungicides. Biological control using endophyte bacteria was conducted to suppress the growth of G. boninense (Bivi et al, 2010;Suryanto et al, 2012). However, applications in the field need consistency in eradicating of G. boninense, because of differences environmental.…”

EXAMINATION OF CHITINOLYTIC BACTERIA IN ALGINATE-CHITOSAN ENCAPSULATION ON CHILI SEED AGAINST DAMPING OFF CAUSED BY <i>FUSARIUM OXYSPORUM</i>

“…raphani, Phytophthora cinnamomi, P. nicotianae and Rhizoctonia solani (Kavraulakis et al, 2010). Our previous studies also showed that chitinolytic bacterial isolates reduce disease incidence of G. boninense in oil palm seedling (Suryanto et al, 2012) and inhibited some plant pathogenic fungal growth (Suryanto et al, 2011).…”

Purification, characterization, and bioassay of putative protease inhibitors from Hevea brasiliensis latex

“…The bioassay of fungal activity was performed using modified technique from Suryanto et al (2012). The outer colony of pure fungal cultures were taken using a cork borer with a diameter of 1 cm and inoculated on potato dextrose agar (PDA) media.…”