A possible novel link between organophosphorus and DDT insecticide resistance genes in Anopheles: Supporting evidence from fenitrothion metabolism studies

Hemingway, Janet; Miyamoto, Junshi; Herath, P. R. J.

doi:10.1016/0048-3575(91)90213-6

Cited by 84 publications

(47 citation statements)

References 7 publications

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“…In contrast, several previous studies showed significant elevation in the activity of esterases implicated in the resistance to OP insecticides [20,[29][30][31][32][33][34][35]. Likewise, several studies reported the implication of GST in OPs resistance in mosquitoes including Culex and Anopheles [36][37][38][39][40]. Our resulted mentioned the minor role of CYP450 in the recorded chlorpyrifos resistance.…”

Section: Discussioncontrasting

confidence: 52%

Evaluation of Chlorpyrifos Resistance and Biochemical Mechanisms of Culex pipiens in Five Localities of Grand Tunis Area, Northeast Tunisia

Daaboub¹,

Tabbabi²,

Lamari³

et al. 2017

Hereditary Genet

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Five Culex pipiens samples were collected at preimaginal stages from breeding sites in 5 localities of Grand Tunis area, Northeast Tunisia, between March 2002 and November 2005. Larvae were used for bioassays using ethanol solutions of chlorpyrifos and propoxur insecticides. All samples were resistant to chlorpyrifos (RR>1, p<0.05). The highest resistance levels (>1,000-fold) were recorded in samples # 2, 4, and 5. Our synergist study showed that the increased detoxification by EST (and/or GST) had only a minor role in the chlorpyrifos resistance, although several overproduced esterases, known to be involved in the OPs resistance, were detected in all the resistant field samples. The mortality due to propoxur was significantly correlated with the LC 50 of chlorpyrifos and indicated an insensitive AChE.

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Section: Discussioncontrasting

confidence: 52%

Evaluation of Chlorpyrifos Resistance and Biochemical Mechanisms of Culex pipiens in Five Localities of Grand Tunis Area, Northeast Tunisia

Daaboub¹,

Tabbabi²,

Lamari³

et al. 2017

Hereditary Genet

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“…Our and previous study confirmed the presence of stronger esterase activity in the most resistant chlorpyrifos samples (samples # 1 and 2) [8,18,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39]. The esterase activity is very variable between individuals of the same sample, as evidenced by the results observed on single individuals after starch gel electrophoresis of the extracts, but it is clear that the proportion of individuals with high esterase activity is more important in samples where resistance is high.…”

Section: Discussionsupporting

confidence: 73%

Susceptibility of Larval Stage of Mosquito Culex pipiens against Chlorpyrifos Insecticide in Southern Tunisia

Daaboub¹,

Tabbabi²,

Cheikh³

et al. 2017

Hereditary Genet

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Three populations of mosquito Culex pipiens were used to study the susceptibility of larval stage against organophosphate chlorpyrifos and carbamate propoxur insecticides in Southern Tunisia. All samples were resistant to chlorpyrifos (RR>1, p<0.05) and the tolerance to this insecticide was varied between 1.8 and 1318. The sample # 3 was the most susceptible and disclosed a little difference when compared to S-Lab (RR50=1.9). The highest resistance levels (>1,000-fold) was recorded in samples # 2 (South East). The level of resistance was lower, not exceeding 2-fold in samples # 3 (Far South). Total esterase activity of field populations detected one or several esterases in all the studied samples, with the exception of the sample # 3 despite the addition of DEF showed that the increased detoxification by EST and/or GST was not responsible for chlorpyrifos resistance in these samples. Oxidative metabolism accounted for only a small part of the observed resistances because chlorpyrifos resistance ratios remained high in the presence of the Pb. The mortality due to propoxur was significantly correlated with the LC50 of chlorpyrifos and indicated an insensitive AChE in samples # 2 which showed the highest resistance levels to chlorpyrifos insecticide (1%). Results were discussed in relation to resistance mechanisms and Culex pipiens control.

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“…The two isoforms present in both species are certainly responsible for the detoxification of common xenobiotics while the isoform only present in the aphidophagous species are probably an adaptation to cope with chemicals specific to its environment and prey. The compounds frequently encountered by S. ribesii include pesticides that are known to induce GST activity (Clark et al, 1986;Hemingway et al, 1991;Kostaropoulos et al, 2001). Other compounds that this species has to face are plant allelochemicals.…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of glutathione S-transferases from two syrphid flies (Syrphus ribesii and Myathropa florae)

Vanhaelen

Francis

Haubruge

2004

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Glutathione S-transferases (GST) play an important role in the detoxification of many substances including organic pollutants and plant secondary metabolites. We compared the GST of two syrphid species, the aphidophagous Syrphus ribesii and the saprophagous Myathropa florea to assess the relation between feeding type and GST patterns. Differences between the GST of the hoverfly species were observed after purification by affinity chromatography, SDS-PAGE and kinetic studies. While the specific activities of the purified enzymes were different, the purification yields were similar. The variation in specific activities was related to the presence of different isoenzymes in both syrphid species by SDS-PAGE. While two bands of 24 and 32 kDa were observed for M. florea, one more band of 26 kDa was present in S. ribesii. When a range of substrate and glutathione concentrations was tested, differences in K and V between the m max glutathione S-transferases from both hoverfly species were also observed. These results are discussed in terms of adaptations to the feeding habit and the habitat of the two syrphid species. ᮊ

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A possible novel link between organophosphorus and DDT insecticide resistance genes in Anopheles: Supporting evidence from fenitrothion metabolism studies

Cited by 84 publications

References 7 publications

Evaluation of Chlorpyrifos Resistance and Biochemical Mechanisms of Culex pipiens in Five Localities of Grand Tunis Area, Northeast Tunisia

Evaluation of Chlorpyrifos Resistance and Biochemical Mechanisms of Culex pipiens in Five Localities of Grand Tunis Area, Northeast Tunisia

Susceptibility of Larval Stage of Mosquito Culex pipiens against Chlorpyrifos Insecticide in Southern Tunisia

Purification and characterization of glutathione S-transferases from two syrphid flies (Syrphus ribesii and Myathropa florae)

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