A possible relationship between the fatty acid composition of yeasts and the ‘petite’ mutation

Johnson, B.; Brown, C. M.

doi:10.1007/bf02328085

Cited by 24 publications

(16 citation statements)

References 18 publications

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“…p values were calculated using a Mann-Whitney U test. p values were corrected for multiple hypotheses post hoc using the Benjamini and Hochberg false discovery rate (26 Equation 3 was calculated based on the fact that that S. pombe only synthesizes saturated and mono-unsaturated fatty acids (27). In the case where fatty acid saturation was determined, lipid species were hydrolyzed in silico prior to normalization (25).…”

Section: Methodsmentioning

confidence: 99%

Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast

Burr

Stewart

Shao

et al. 2016

Journal of Biological Chemistry

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Eukaryotic lipid synthesis is oxygen-dependent with cholesterol synthesis requiring 11 oxygen molecules and fatty acid desaturation requiring 1 oxygen molecule per double bond. Accordingly, organisms evaluate oxygen availability to control lipid homeostasis. The sterol regulatory element-binding protein (SREBP) transcription factors regulate lipid homeostasis. In mammals, SREBP-2 controls cholesterol biosynthesis, whereas SREBP-1 controls triacylglycerol and glycerophospholipid biosynthesis. In the fission yeast Schizosaccharomyces pombe, the SREBP-2 homolog Sre1 regulates sterol homeostasis in response to changing sterol and oxygen levels. However, notably missing is an SREBP-1 analog that regulates triacylglycerol and glycerophospholipid homeostasis in response to low oxygen. Consistent with this, studies have shown that the Sre1 transcription factor regulates only a fraction of all genes up-regulated under low oxygen. To identify new regulators of low oxygen adaptation, we screened the S. pombe nonessential haploid deletion collection and identified 27 gene deletions sensitive to both low oxygen and cobalt chloride, a hypoxia mimetic. One of these genes, mga2, is a putative transcriptional activator. In the absence of mga2, fission yeast exhibited growth defects under both normoxia and low oxygen conditions. Mga2 transcriptional targets were enriched for lipid metabolism genes, and mga2⌬ cells showed disrupted triacylglycerol and glycerophospholipid homeostasis, most notably with an increase in fatty acid saturation. Indeed, addition of exogenous oleic acid to mga2⌬ cells rescued the observed growth defects. Together, these results establish Mga2 as a transcriptional regulator of triacylglycerol and glycerophospholipid homeostasis in S. pombe, analogous to mammalian SREBP-1.

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Section: Methodsmentioning

confidence: 99%

Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast

Burr

Stewart

Shao

et al. 2016

Journal of Biological Chemistry

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“…Yeasts generally abound in unsaturated fatty acids, 18:1 (oleic acid) being one of the major components (Rattray et al, 1975). Polyunsaturated acids, 18:2 (linoleic acid) and 18:3 (linolenic acid), are also usually associated with yeast strains (Johnson and Brown, 1972). Figure 4 also shows the effect of various C 18 unsaturated acids differing in the number of double bonds on the antifungal activity of compound 1.…”

Section: Resultsmentioning

confidence: 96%

Antimicrobial triterpenes from Ilex integra and the mechanism of antifungal action

et al. 1999

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Antimicrobial triterpenes were isolated from the fruits of Ilex integra. Their structures were elucidated by spectral data and identified as rotundic acid (1), ulsolic acid (2) and peduncloside (3). Triterpene 1 showed significantly broad antimicrobial activity against bacteria, yeast and filamentous fungi. The antifungal activity of 1 was reversed by fatty acids. Cellular constituents leaked from Candida albicans cells incubated with triterpene 1. These results suggest that the antimicrobial activity of triterpenes in I. integra is due to a change of membrane permeability arising from membrane lipid alteration.

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“…If polyunsaturated fatty acids were detected in cells, they would only arise from the culture medium. Moreover, this characteristic seems to be related to the ability to yield respiratorydeficient (petite) mutants on treatment with acriflavine (Johnson and Brown 1972) and particularly with repression of respiration by fermentation (Crabtree effect, De Deken 1966).…”

Section: Discussionmentioning

confidence: 97%

“…This yeast is at present indexed as Torulaspora delbrueckii (Barnett et a1 1983). This species seems to be characterised by a heterogeneous fatty acid profile (Johnson and Brown 1972;Kaneko et a1 1976) unless the classification integrates physiologically dissimilar yeasts. MalfeitoFerreira et a1 (1989) emphasise the fact that T delbrueckii may be identified by comparing their profiles.…”

Section: Discussionmentioning

confidence: 98%

Differentiation between fermenting and spoilage yeasts in wine by total free fatty acid analysis

et al. 1992

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Differentiation between fermenting and spoilage yeasts in wine was estimated by cellular fatty acid profiles. Forty-two strains of yeasts representing 17 genera were grown on a defined liquid medium for 48 h at 25OC in a rotary shaker. After saponification of yeast cells, free fatty acid extracts were analysed by gas chromatography. Multivariate analysis was performed by Principal Components Analysis (PCA) to define clusters of fatty acids and yeasts.Strains were characterised especially by long-chain fatty acids, palmitic (C16) to linolenic (C18 : 3) acid under aerobic culture. Nevertheless, most of Saccharomyces cerevisiae and also Dekkera bruxellensis (former names D intermedia and Brettunomyces lumbicus) synthesized medium-chain fatty acids, octanoic (C8) acid to dodecanoic (C12) acid. With this method it was possible to differentiate fermenting grape yeasts such as S cerevisiae from spoilage yeasts on the basis of the absence of linoleic (C18 : 2) and linolenic (C18 : 3) acids. However, the method seemed unreliable for the identification of strains, more particularly those of species of S cerevisiae.

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A possible relationship between the fatty acid composition of yeasts and the ‘petite’ mutation

Cited by 24 publications

References 18 publications

Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast

Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast

Antimicrobial triterpenes from Ilex integra and the mechanism of antifungal action

Differentiation between fermenting and spoilage yeasts in wine by total free fatty acid analysis

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