A Possible Role of Charged Amino-Acid Clusters on the Surface of Cysteine Proteases for Preserving Activity when Binding with Polymers

Pankova, S. M.; Sakibaev, F. A.; Holyavka, M. G.; Artyukhov, V. G.

doi:10.1134/s0006350922010146

Cited by 2 publications

(1 citation statement)

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“…With the advancements in bioinformatics and computational biology, the identification of interaction mechanisms between enzymes and immobilization matrices, as well as their effects on enzyme structure, has become more accessible. Molecular docking methods have made it possible to unveil the interaction mechanisms down to the specific amino acid residue and the characteristic type of interaction [34][35][36][37]. However, due to the imperfections of simulation algorithms and the absence of a complete macromolecule model, in silico results should be validated with experimental data.…”

Section: Introductionmentioning

confidence: 99%

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Сорокин

Goncharova

Lavlinskaya

et al. 2023

IJMS

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This study investigates the features of interactions between cysteine proteases (bromelain, ficin, and papain) and a graft copolymer of carboxymethyl cellulose sodium salt with N-vinylimidazole. The objective is to understand the influence of this interactions on the proteolytic activity and stability of the enzymes. The enzymes were immobilized through complexation with the carrier. The interaction mechanism was examined using Fourier-transform infrared spectroscopy and flexible molecular docking simulations. The findings reveal that the enzymes interact with the functional groups of the carrier via amino acid residues, resulting in the formation of secondary structure elements and enzyme’s active sites. These interactions induce modulation of active site of the enzymes, leading to an enhancement in their proteolytic activity. Furthermore, the immobilized enzymes demonstrate superior stability compared to their native counterparts. Notably, during a 21-day incubation period, no protein release from the conjugates was observed. These results suggest that the complexation of the enzymes with the graft copolymer has the potential to improve their performance as biocatalysts, with applications in various fields such as biomedicine, pharmaceutics, and biotechnology.

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Section: Introductionmentioning

confidence: 99%