A possible role of S-nitrosothiols at the nitrergic relaxations in the mouse corpus cavernosum

Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum

“…On the other hand, pyrogallol, a superoxide generating agent (22) and putative indirect nitric oxide scavenger (16), did not affect relaxations induced by S-NONOate or SNP. These findings are consistent with our previous studies (16). It was suggested that pyrogallol could not penetrate into cells (23).…”

“…Hydroxocobalamin (15,16,21) significantly inhibited the relaxations to S-NONOate and SNP. This finding may suggest that direct NO-dependent sGC activation by S-NONOate plays a critical role in the relaxant activity of the substance.…”

“…After the equilibration period, the tissue was treated with 5 µM of phenylephrine. The active tone reached a stable level within 5 min; at the end of this period, EFS (2,4,8,16, and 32 Hz: 30 V, 0.5 ms) was applied to the tissue for 15 s by a Grass S88 stimulator via 2 parallel platinum electrodes embedded in Perspex. We used the same EFS parameters in our previously published papers (11,15).…”

“…In some experiments, after the first series of responses were recorded, the tissues were incubated with S-NONOate (1 and 5 µM) for 30 min and then the second series of relaxation responses to EFS were examined. In another group, we evaluated the effect of S-NONOate on the neurogenic contractile responses to EFS (2,4,8,16, and 32 Hz: 30 V, 0.5 ms) in the presence of L-NOARG (100 µM). In these experiments, L-NOARG was always present in the bathing medium to inhibit nitric oxide synthase enzyme.…”

“…Mouse corpus cavernosum has been used previously for in vivo and in vitro studies of erectile mechanisms (14)(15)(16)(17)(18). Therefore, in the present study, we aimed to demonstrate the pharmacological effects of S-NONOate in the mouse cavernous tissue.…”