A possible role of tachykinin-related peptide on an immune system activity of mealworm beetle, Tenebrio molitor L.

Urbański, Arkadiusz; Konopińska, N.; Lubawy, Jan; Walkowiak-Nowicka, Karolina; Marciniak, Paweł; Rolff, Jens

doi:10.1016/j.dci.2021.104065

Cited by 6 publications

(22 citation statements)

References 59 publications

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“…We also identified and predicted the sequence and structure of the TRP receptor (TRPR) and confirmed the presence of gene expression encoding TRPR in Tenebrio haemocytes. This result supports the notion that TRPs directly influence the activity of insect haemocytes 9 . In addition, research conducted by Kamareddine et al 11 showed that the innate immune deficiency (IMD) pathway can regulate TRP transcription in the Drosophila melanogaster gut.…”

Section: Introductionsupporting

confidence: 90%

“…Our previous research provided the first evidence for the possible role of TRPs in regulating the insect immune system in the mealworm beetle Tenebrio molitor 9 , a storage pest species. The application of Tenmo-TRP-7 (one of the TRPs identified in T. molitor ) elicited many physiological effects, resulting in an increase in the total number of circulating haemocytes, a decrease in the number of phagocytic immune cells, and altered haemocyte adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…The application of Tenmo-TRP-7 (one of the TRPs identified in T. molitor ) elicited many physiological effects, resulting in an increase in the total number of circulating haemocytes, a decrease in the number of phagocytic immune cells, and altered haemocyte adhesion. Moreover, Tenmo-TRP-7 enhanced the activity of phenoloxidase (PO) in T. molitor haemolymph 9 , which is one of the main components of the insect immune system 10 . The injection of Tenmo-TRP-7 also reduced the DNA damage observed in haemocytes 9 .…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Tenmo-TRP-7 enhanced the activity of phenoloxidase (PO) in T. molitor haemolymph 9 , which is one of the main components of the insect immune system 10 . The injection of Tenmo-TRP-7 also reduced the DNA damage observed in haemocytes 9 . We also identified and predicted the sequence and structure of the TRP receptor (TRPR) and confirmed the presence of gene expression encoding TRPR in Tenebrio haemocytes.…”

Section: Introductionmentioning

confidence: 99%

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Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Urbański

Johnston

Bittermann

et al. 2022

Sci Rep

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Tachykinins (TKs) are a group of conserved neuropeptides. In insects, tachykinin-related peptides (TRPs) are important modulators of several functions such as nociception and lipid metabolism. Recently, it has become clear that TRPs also play a role in regulating the insect immune system. Here, we report a transcriptomic analysis of changes in the expression levels of immune-related genes in the storage pest Tenebrio molitor after treatment with Tenmo-TRP-7. We tested two concentrations (10–8 and 10–6 M) at two time points, 6 and 24 h post-injection. We found significant changes in the transcript levels of a wide spectrum of immune-related genes. Some changes were observed 6 h after the injection of Tenmo-TRP-7, especially in relation to its putative anti-apoptotic action. Interestingly, 24 h after the injection of 10–8 M Tenmo-TRP-7, most changes were related to the regulation of the cellular response. Applying 10–6 M Tenmo-TRP-7 resulted in the downregulation of genes associated with humoral responses. Injecting Tenmo-TRP-7 did not affect beetle survival but led to a reduction in haemolymph lysozyme-like antibacterial activity, consistent with the transcriptomic data. The results confirmed the immunomodulatory role of TRP and shed new light on the functional homology between TRPs and TKs.

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Section: Introductionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Urbański

Johnston

Bittermann

et al. 2022

Sci Rep

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show abstract

“…Between insect TRPs and vertebrate TKs exist not only structural similarities, much research concerning this issue showed a wide spectrum of functional homology of these neuropeptides ( Table 1 ). For example, members of both families participate in the regulation of visceral muscle contractions, nociception, and immune system activity [ 11 , 160 ]. In addition, TKs and TRPs probably play a very important role in modulation of lipid metabolism [ 161 , 162 ].…”

Section: Peptides–neuropeptides–hormonesmentioning

confidence: 99%

Insects as a New Complex Model in Hormonal Basis of Obesity

Walkowiak-Nowicka

Chowański

Urbański

et al. 2021

IJMS

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Nowadays, one of the biggest problems in healthcare is an obesity epidemic. Consumption of cheap and low-quality energy-rich diets, low physical activity, and sedentary work favor an increase in the number of obesity cases within many populations/nations. This is a burden on society, public health, and the economy with many deleterious consequences. Thus, studies concerning this disorder are extremely needed, including searching for new, effective, and fitting models. Obesity may be related, among other factors, to disrupting adipocytes activity, disturbance of metabolic homeostasis, dysregulation of hormonal balance, cardiovascular problems, or disorders in nutrition which may lead to death. Because of the high complexity of obesity, it is not easy to find an ideal model for its studies which will be suitable for genetic and physiological analysis including specification of different compounds’ (hormones, neuropeptides) functions, as well as for signaling pathways analysis. In recent times, in search of new models for human diseases there has been more and more attention paid to insects, especially in neuro-endocrine regulation. It seems that this group of animals might also be a new model for human obesity. There are many arguments that insects are a good, multidirectional, and complex model for this disease. For example, insect models can have similar conservative signaling pathways (e.g., JAK-STAT signaling pathway), the presence of similar hormonal axis (e.g., brain–gut axis), or occurrence of structural and functional homologues between neuropeptides (e.g., neuropeptide F and human neuropeptide Y, insulin-like peptides, and human insulin) compared to humans. Here we give a hint to use insects as a model for obesity that can be used in multiple ways: as a source of genetic and peptidomic data about etiology and development correlated with obesity occurrence as well as a model for novel hormonal-based drug activity and their impact on mechanism of disease occurrence.

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Functional homology of tachykinin signalling: The influence of human substance P on the immune system of the mealworm beetle, Tenebrio molitor L.

Urbański

Konopińska

Walkowiak-Nowicka

et al. 2023

Developmental & Comparative Immunology

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A possible role of tachykinin-related peptide on an immune system activity of mealworm beetle, Tenebrio molitor L.

Cited by 6 publications

References 59 publications

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Insects as a New Complex Model in Hormonal Basis of Obesity

Functional homology of tachykinin signalling: The influence of human substance P on the immune system of the mealworm beetle, Tenebrio molitor L.

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