A potato late blight resistance gene protects against multiple Phytophthora species by recognizing a broadly conserved RXLR-WY effector

Lin, Xiao; Olave-Achury, Andrea; Heal, Robert; Pais, Marina; Witek, Kamil; Ahn, Hyung‐Joon; Zhao, He; Bhanvadia, Shivani; Karki, Hari S.; Song, Tianqiao; Wu, Chih‐Hang; Adachi, Hiroaki; Kamoun, Sophien; Vleeshouwers, Vivianne G. A. A.; Jones, Jonathan D. G.

doi:10.1016/j.molp.2022.07.012

Cited by 41 publications

(49 citation statements)

References 57 publications

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“…The HR index (strong HR = 2, weak HR = 1, no HR = 0, NA = not available) was used for the heatmap. These effectors were screened on N. benthamiana 30 , their recognitions are included in this heatmap. Empty PVX vector was used as negative control, co-expression of Rpi-amr3::HF and AVRamr3::GFP was used as positive control.…”

Section: Resultsmentioning

confidence: 99%

“…Both Rpi-amr1 and Rpi-amr3 can recognize the AVRamr1 and AVRamr3 homologs from other Phytophthora pathogens, and Rpi-amr3 confers resistance against P. parasitica and P. palmivora by detecting their conserved AVRamr3 homologs 29,30 . Conceivably, Rpi-amr4, Rpi-amr16 and Rpi-amr17 might also recognize the effectors from other important Phytophthora pathogens and confer resistance against multiple diseases.…”

Section: Discussionmentioning

confidence: 99%

“…2e). Rpi-amr3 and Rpi-amr1 require NRC2/3/4 and NRC2/3, respectively 29,30 . However, NRC1 is missing in N. benthamiana 41 .…”

Section: Defining the S Americanum Pan-nlr Repertoirementioning

confidence: 99%

“…Diploid Solanum americanum and its closely related hexaploid descendant S. nigrum are both highly resistant to LB. Previously, our group cloned Rpi-amr1 and Rpi-amr3 from different resistant S. americanum accessions, and their functional homologs were cloned from S. nigrum 25,29,30 . Both Rpi-amr1 and Rpi-amr3 confer late blight resistance in potato.…”

Section: Introductionmentioning

confidence: 99%

“…Both Rpi-amr1 and Rpi-amr3 confer late blight resistance in potato. The cognate effectors AVRamr1 and AVRamr3 were identified by pathogen enrichment sequencing (PenSeq), and effectoromics screening 30,31 .…”

Section: Introductionmentioning

confidence: 99%

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TheSolanum americanumpangenome and effectoromics reveal new resistance genes against potato late blight

Lin

Jia

Heal

et al. 2022

Preprint

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Late blight caused by the oomycete pathogen Phytophthora infestans continues to cause major worldwide losses in potato and tomato. Most accessions of Solanum americanum, a globally distributed, wild Solanaceae plant, are highly resistant to late blight. We generated high-quality reference genomes of four S. americanum accessions, re-sequenced 52 accessions, and we defined variation in the NLR immune receptor genes (the S. americanum NLRome). We further screened for variation in recognition of ~315 P. infestans RXLR effectors in 52 S. americanum accessions. Using these genotypic and phenotypic data, we cloned three novel NLR-encoding genes Rpi-amr4, Rpi-amr16 and Rpi-amr17, and determined their corresponding RXLR effector genes Avramr4 (PITG_22825), Avramr16 (PITG_02860) and Avramr17 (PITG_04373) from P. infestans. These genomic resources and methodology will support efforts to convert potato into a nonhost of late blight and can be applied to diseases of other crops.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…2e). Rpi-amr3 and Rpi-amr1 require NRC2/3/4 and NRC2/3, respectively 29,30 . However, NRC1 is missing in N. benthamiana 41 .…”

Section: Defining the S Americanum Pan-nlr Repertoirementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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TheSolanum americanumpangenome and effectoromics reveal new resistance genes against potato late blight

Lin

Jia

Heal

et al. 2022

Preprint

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New genomic techniques can contribute to reduced pesticide usage in Europe

Sundström,

Berlin,

Phuong

et al. 2024

Plants People Planet

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Societal Impact StatementCan modern breeding technologies, such as genome editing, contribute to reduced pesticide usage? This question has been accentuated by a recent legal proposal to exempt genome‐edited plants from the strict regulations applied to classical genetically modified (GM) crops within the European Union (EU). Using official statistics on crop cultivation and pesticide usage for two example crops commonly grown in Sweden, we calculate that cereal farmers collectively could save up to 70 million € in pesticide usage for wheat alone and that a late blight‐resistant potato could reduce pesticide usage by over 80% provided that the EU legislation is amended.SummaryThe European Commission has set goals to reduce the use of chemical pesticides, and one way to meet these goals in the agricultural sector is to breed disease‐resistant crops. Here, we ask whether modern breeding technologies, for example, genome editing using site‐directed nucleases, can contribute to these goals. This question has been accentuated by recent legal proposals in the European Union (EU) and several other jurisdictions worldwide to exempt genome‐edited plants from the strict regulations often applied to classical genetically modified (GM) crops. Using official statistics on crop cultivation and pesticide usage for two example crops commonly grown in Sweden (wheat and potato), we show that cereal farmers collectively could potentially save up to 70 million € in pesticide usage for wheat alone and that a late blight‐resistant potato could reduce pesticide usage by over 80% provided that the EU legislation is amended. Given the immense potential of genome‐edited crops, we further discuss details in the legal proposal currently being negotiated in the EU on the so‐called new genomic techniques that includes both genome editing and targeted insertions of cisgenes. Although promising, we argue that several technical limitations in the legal proposal will, if implemented, hamper the development of disease‐resistant crops and make the suggested legislation less future‐proof.

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The effectors of Phytophthora infestans impact host immunity upon regulation of antagonistic hormonal activities

Wang,

Su,

et al. 2023

Planta

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A potato late blight resistance gene protects against multiple Phytophthora species by recognizing a broadly conserved RXLR-WY effector

Cited by 41 publications

References 57 publications

TheSolanum americanumpangenome and effectoromics reveal new resistance genes against potato late blight

TheSolanum americanumpangenome and effectoromics reveal new resistance genes against potato late blight

New genomic techniques can contribute to reduced pesticide usage in Europe

The effectors of Phytophthora infestans impact host immunity upon regulation of antagonistic hormonal activities

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