A potent antifungal rhizobacteria Bacillus velezensis RB.DS29 isolated from black pepper (Piper nigrum L.)

Trinh, Thi Huyen Trang; Wang, San-Lang; Nguyen, Van Bon; Tran, Minh D.; Doan, Chien Thang; Vo, Thi Phuong Khanh; Huynh, Que V.; Nguyen, Anh Dzung

doi:10.1007/s11164-019-03971-5

Cited by 36 publications

(34 citation statements)

References 39 publications

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“…In this study, the results show a positive correlation between chitinase activity and the Phytophthora antagonism activity (r = 0.36, n = 18), but a negative correlation between protease activity and the Phytophthora antagonism activity (r = −0.23, n = 18). This result appears to be same as results of recent works by , who also indicated that proteases, chitinases, and beta glucanses produced by rhizobacteria of black pepper showed no effect on Phytophthora antagonism [16]. Table 3.…”

Section: In Vivo Phytophthora Antagonism By the Endophytic Bacteriasupporting

confidence: 91%

“…It is reported that diversity of endophytic bacteria depends on the host, such as genus, species, and varieties by interaction and adaptation between plant hosts and the bacteria [30]. The diversity of endophytic bacteria community in this study was the same diversity as reported by Trinh et al [16] who indicated that there were approximately 500 rhizobacteria isolated from the root of black pepper collected at five provinces of the Central Highlands, Vietnam. Nguyen et al [12] isolated endophytic bacteria from the roots of black pepper cultivated in the Central Highlands that were screened for plant growth-promoting activity.…”

Section: Isolation and Morphology Of Endophytic Bacteriasupporting

confidence: 82%

“…In regard to the protease activity, the highest was found in EB.DC6 (7.880 U/mL) and then EB.CP36 (7.372 U/mL), EB.DL1 (4.218 U/mL), and EB.KN13 (3.965 U/mL). Generally, chitinase activity of the endophytic bacterial isolates was of 0.154-0.507 U/mL and protease activity from 1.33-7.37 U/mL, the same as those from the rhizobacteria isolated from roots of black pepper [12,16]. In this study, the results show a positive correlation between chitinase activity and the Phytophthora antagonism activity (r = 0.36, n = 18), but a negative correlation between protease activity and the Phytophthora antagonism activity (r = −0.23, n = 18).…”

Section: In Vivo Phytophthora Antagonism By the Endophytic Bacteriamentioning

confidence: 68%

“…Phytophthora is the most common fungus associated with root rot disease of agricultural crops, such as tomato, potato, onion, coffee, rubber, pepper, and black pepper [13][14][15]. Root rot disease caused by Phytophthora is one of the sources of serious production reduction in black pepper in Vietnam, India, Malaysia, and other countries [16,17]. Using chemical fungicides to manage Phytophthora in black pepper production is the most common application.…”

Section: Introductionmentioning

confidence: 99%

“…Nguyen et al [12] isolated 106 endophytic isolates and found that Bacillus amyloliquefaciens EB.EK2 produces seven potent biochemical compounds for Phytophthora resistance. Trinh et al [16] found a promising antifugal rhizobacteria Bacillus velezensis RB. DS29.…”

Section: Introductionmentioning

confidence: 99%

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Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

et al. 2020

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In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45-11.21%) and lower fatal rate (11.11-15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

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Section: In Vivo Phytophthora Antagonism By the Endophytic Bacteriasupporting

confidence: 91%

Section: Isolation and Morphology Of Endophytic Bacteriasupporting

confidence: 82%

Section: In Vivo Phytophthora Antagonism By the Endophytic Bacteriamentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

et al. 2020

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Isolation, Plant Growth‐Promoting Properties, and Whole‐Genome Sequence of a Novel Paenibacillus Species

Tran,

Huynh,

et al. 2024

Journal of Basic Microbiology

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This work aimed to isolate and characterize a novel chitin‐degrading bacterium from Yok Don National Park, Vietnam, for crop production studies. Among the chitinolytic isolates, strain YSY‐4.3 was selected, which grew rapidly and produced a large halo around the colony. 16S rDNA analysis indicated that the strain is a novel species in the genus Paenibacillus, and an in vitro evaluation showed that the strain produced phytohormones (IAA, GA3, and zeatin), biofilms, and siderophores; possessed cellulase; and exerted antifungal activity. The whole genome of the strain was 5,628,400 bp with 49.3% GC content, 5056 coding sequences, 48 tRNA, and 1 rRNA. It shared the highest values of digital DNA–DNA hybridization (67.4%) and average nucleotide identity (89.54%) with those of Paenibacillus woosongensis B2_4 (CP126084.1), suggesting a novel species. Of the coding sequences, 4287 proteins were identified by COG, and 2561 were assigned by KEGG. The genome contained at least 51 genes involved in plant growth and resistance to heavy‐metal toxicity and 359 carbohydrate‐active enzymes. The chitinolytic system of the strain was composed of 15 enzymes, among them, PsChiC, which contained a GH18 catalytic domain and a GH5 catalytic domain, had not been previously reported. In addition, the genome possessed 15 gene clusters encoding antimicrobial metabolites, 10 of which are possible novel clusters. This study expands knowledge regarding novel chitinolytic bacteria from Yok Don National Park and provides a valuable gene resource for future studies.

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Phytostimulation and biocontrol potential of Gram-positive endospore-forming Bacilli

Soni

Keharia

2021

Planta

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A potent antifungal rhizobacteria Bacillus velezensis RB.DS29 isolated from black pepper (Piper nigrum L.)

Cited by 36 publications

References 39 publications

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Isolation, Plant Growth‐Promoting Properties, and Whole‐Genome Sequence of a Novel Paenibacillus Species

Phytostimulation and biocontrol potential of Gram-positive endospore-forming Bacilli

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