A potential biomarker of esophageal squamous cell carcinoma WTAP promotes the proliferation and migration of ESCC

Luo, Gaomeng; Qi, Yao; Lei, ZhengYao; Shen, XiaoYing; Chen, MingMin; Li, Du; Wu, Chong; Bo, Jiaqi; Wang, ShunLi; Zhao, Jun; Yi, Xianghua

doi:10.1016/j.prp.2022.154114

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…Interfering with METTL3 expression can reduce the level of m6A modification of HIF1α and inhibit the metabolic reprogramming of colorectal and liver cancer cells [ 48 , 49 ]. The elevated expressions of WTAP and VIRMA genes were strongly correlated with poor prognosis in ESCA patients, augmented levels of WTAP were associated with poor OS of EA [ 50 ], whereas WTAP promoted the proliferation and migration of ESCC [ 51 ]. Both METTL3 and WTAP accelerates the Warburg effect of gastric cancer [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP

Gao

Yuan

et al. 2023

J Transl Med

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Background Cytoplasmic activation/proliferation-associated protein-1 (Caprin-1) is implicated in cancer cell proliferation and tumorigenesis; however, its role in the development of esophageal carcinoma (ESCA) has not been examined. Methods Biological methods and data analysis were used to investigate the expression of Caprin-1 in ESCA tissue and cell lines. We comprehensively analyzed the mRNA expression and prognostic values, signalling pathways of CAPRIN1 in ESCA using public databases online. Biological functions of CAPRIN1 were performed by clorimetric growth assay, EdU staining, colony formation, flow cytometry, apoptosis analysis, Western blot, lactate detection assay, extracellular acidification rates. The underlying mechanism was determined via flow cytometric analysis, Western blot and rescue experiments. In addition, xenograft tumor model was constructed to verify the phenotypes upon CAPRIN1 silencing. Results Caprin-1 expression was significantly elevated in both ESCA tumor tissues and cell lines compared with that in normal adjacent tissues and fibroblasts. Increased CAPRIN1 mRNA expression was significantly associated with clinical prognosis and diagnostic accuracy. The GO enrichment and KEGG pathway analysis CAPRIN1 might be related to immune-related terms, protein binding processes, and metabolic pathways. A significant positive correlation was observed between high Caprin-1 protein levels and lymph node metastasis (P = 0.031), ki-67 (P = 0.023), and 18F- FDG PET/CT parameters (SUVmax (P = 0.002) and SUV mean (P = 0.005)) in 55 ESCA patients. At cut-off values of SUVmax 17.71 and SUVmean 10.14, 18F- FDG PET/CT imaging predicted Caprin-1 expression in ESCA samples with 70.8% sensitivity and 77.4% specificity. In vitro and in vivo assays showed that Caprin-1 knockdown affected ESCA tumor growth. Silencing Caprin-1 inhibited ESCA cell proliferation and glycolysis, and decreased the expression of methyltransferase-like 3 (METTL3) and Wilms’ tumor 1-associating protein (WTAP). However, this effect could be partially reversed by the restoration of METTL3 and WTAP expression. Conclusions Our data suggest that Caprin-1 could serve as a prognostic biomarker and has an oncogenic role in ESCA.

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Section: Discussionmentioning

confidence: 99%

Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP

Gao

Yuan

et al. 2023

J Transl Med

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The METTL3-m6A-YTHDC1-AMIGO2 axis contributes to cell proliferation and migration in esophageal squamous cell carcinoma

Qiu,

Tian,

Miao

et al. 2024

Gene

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Biomarkers of lymph node metastasis in esophageal cancer

Wu,

Cai,

Jiang

et al. 2024

Front. Immunol.

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Esophageal cancer (EC) is among the most aggressive malignancies, ranking as the seventh most prevalent malignant tumor worldwide. Lymph node metastasis (LNM) indicates localized spread of cancer and often correlates with a poorer prognosis, emphasizing the necessity for neoadjuvant systemic therapy before surgery. However, accurate identification of LNM in EC presents challenges due to the lack of satisfactory diagnostic techniques. Imaging techniques, including ultrasound and computerized tomography scans, have low sensitivity and accuracy in assessing LNM. Additionally, the existing serological detection lacks precise biomarkers. The intricate and not fully understood molecular processes involved in LNM of EC contribute to current detective limitations. Recent research has shown potential in using various molecules, circulating tumor cells (CTCs), and changes in the microbiota to identify LNM in individuals with EC. Through summarizing potential biomarkers associated with LNM in EC and organizing the underlying mechanisms involved, this review aims to provide insights that facilitate biomarker development, enhance our understanding of the underlying mechanisms, and ultimately address the diagnostic challenges of LNM in clinical practice.

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A potential biomarker of esophageal squamous cell carcinoma WTAP promotes the proliferation and migration of ESCC

Cited by 4 publications

References 23 publications

Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP

Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP

The METTL3-m6A-YTHDC1-AMIGO2 axis contributes to cell proliferation and migration in esophageal squamous cell carcinoma

Biomarkers of lymph node metastasis in esophageal cancer

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