A potential correlation between adipokines, skeletal muscle function and bone mineral density in middle-aged and elderly individuals

Abstract

Background Evidence exists of a strong association between inflammation and a decrease in skeletal muscle function and bone mineral density (BMD); however, the specific mechanisms of these associations remain unclear. Adipokines, as key regulators of the inflammatory response, may be implicated in these processes. The objective of this study was to explore the potential correlation between adipokines, skeletal muscle function and BMD in middle-aged and elderly individuals. Methods A comparative cross-sectional study was carried out at the Huadong Hospital Affiliated with Fudan University (Shanghai, China). A total of 460 middle-aged and elderly individuals were recruited, and 125 were enrolled in the analysis. Their biochemical indices, body composition, skeletal muscle function and BMD were measured. Bioinformatic analysis was also employed to identify potential adipokine targets linked to skeletal muscle function and BMD. To validate these targets, plasma and peripheral blood mononuclear cells (PBMCs) were harvested from these individuals and subjected to western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). Results Individuals in this cross-sectional study were categorized into 2 groups according to their median skeletal muscle mass (SMM) (28.8 kg for males and 20.6 kg for females). Individuals with lower SMM exhibited poorer grip strength (P = 0.017), longer 5-Times-Sit-to-Stand Test (FTSST) duration (P = 0.029), lower total hip BMD (P = 0.043), lower femoral neck BMD (P = 0.011) and higher levels of inflammatory markers in comparison with individuals with higher SMM. Bioinformatics analysis identified LEP, ADIPOQ, RBP4, and DPP4 as potential adipokine targets associated with skeletal muscle function and BMD. In vitro experiments demonstrated that individuals with decreased skeletal muscle function and BMD expressed higher levels of these adipokines. Conclusions Skeletal muscle function is positively correlated with BMD and negatively correlated with levels of inflammatory markers among middle-aged and elderly individuals. Those with lower skeletal muscle function and BMD tend to have a higher expression of LEP, ADIPOQ, RBP4 and DPP4.

LUSTA Large Channel Endoscopy in the Treatment of Severe Lumbar Spinal Stenosis Complicated with Aneurysm of Ascendingaorta: A Case Report

“…As elderly patients are prone to hypertension, coronary heart disease and other medical diseases, the incidence of aneurysm of ascendingaorta increases. Moreover, for patients with osteoporosis, due to the increase of age and the attachment of calcium ions in bones to the arterial wall, the vascular wall gradually hardens and calcifies [2], and the loss of calcium ions can accelerate the development of osteoporosis, further reduce the elasticity of the ascending aorta and increase the risk of arteriosclerosis [3]. In addition, this patient is accompanied by cardiovascular disease because of severe low back and leg pain, but most doctors miss the diagnosis of aneurysm of ascendingaorta because of medical inertia, so patients must be examined comprehensively to avoid serious accidents during the operation [4].…”