A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin

Abstract

Human Adipose Derived Mesenchymal Stem Cells (HADMSCs) and Human Hair Follicle Derived Mesenchymal Stem Cells (HHFDMSCs) have attracted great interest because of their multilineage differentiation potential, selfrenewal properties, and their possible use of cell and gene therapies. This present study to investigate the neurogenic differentiation ability of hADMSCs and hHFDMSCs induced by Epidermal Growth Factors (EGF), Fibroblast Growth Factor (FGF), Platelet Derived Growth Factor (PDGF) and Forskolin. This study was true experimental with longitudinal study design. The sample size determined with minimal sample size formula and it was randomly chosen. These studies employed an in vitro design for the expansion and proliferation of Mesenchymal Stem Cells (MSCs) and examined the heterogeneity of these cells using the markers CD105, CD90, OCT4, and SOX2. MSCs from adipose tissue and hair follicles were induced with EGF, FGF, PDGF and Forskolin to differentiate and generate neurons. The capacity of MSCs to generate neurons were verified using glial fibrillary acidic protein, nestin, and β-tubulin III. The expression of neural markers and morphological changes in Mesenchymal stem cells from hADMSCs and hHFDMSCs were confirmed. hADMSCs and hHFDMSCs share a similar capacity to differentiate and generate neurons, which is beneficial for the development of neuronal restoration for future therapies for patients suffering from neurological diseases.

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

“…28 The other sources of MSCs, such as those derived from hair follicles, the umbilical cord, adipose tissue, and dental pulp, also positively presented the specific cell surface markers of MSCs. 5,6,8,9,11 GDMSCs have a potential proliferation rate characterized by Oct-4, Nanog, and SOX2 expression that play an important role in the self-renewal and survival of MSCs. 41,42 In addition to the proliferation potential of GDMSCs, their migration capability is also good.…”

“…28 The other sources of MSCs, such as those derived from hair follicles, the umbilical cord, adipose tissue, and dental pulp, also positively presented the specific cell surface markers of MSCs. 5 6 8 9 11 …”

“…The assessment of GDMSCs osteogenic differentiation ability was performed; 1. 10,6 GDMSCs/mL at the fourth passage were cultivated in a plate culture with a culture medium until it reached 80% confluence. Osteogenic differentiation of GDMSCs was induced with an osteogenic medium consisting of 100 nM MEM α, dexamethasone, 200 μM ascorbic acid, and 10 mM of glycerol 2-phosphate (Osteo-MAX; Merck, United States).…”

“…GDMSCs flow cytometry analysis begins with sample preparation; 1. 10,6 GDMSCs/mL dissolved in 3 mL cold (4°C) PBS is put into a tightly closed falcon tube. A 200 μL aliquot of the GDMSCs suspension is put into test tubes as required.…”

“…3 MSCs have been successfully extracted from various tissues, such as the umbilical cord, hair follicles, and adipose tissue, including orofacial tissues. [4][5][6][7][8] MSCs can be derived from orofacial tissue, including the dental pulp of permanent or deciduous teeth, the periodontal ligament, and the gingiva. [9][10][11][12][13] One of the potential and superior sources of MSCs from the oral cavity was in the gingiva.…”