A potentially important resource: endophytic yeasts

Ling, Lijun; Tu, Yixin; Ma, Wenxia; Feng, Shenglai; Yang, Caiyun; Zhao, Yunhua; Wang, Nan; Li, Zibin; Lu, Lu; Ji, Zhang

doi:10.1007/s11274-020-02889-0

Cited by 19 publications

(8 citation statements)

References 36 publications

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“…One of the foremost studied yeast species is Saccharomyces cerevisiae due to the growing interest in industrial processes [ 6 , 7 ]. Endophytic yeasts have a unicellular part of their lifecycle and usually reside within plant tissues and do not cause damage to their hosts [ 8 ]. The endophytes can be locally or systemically distributed in various plants where they are protected from biotic and abiotic stress [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Tobacco Plant Growth-Promoting and Antifungal Activities of Three Endophytic Yeast Strains

Petkova

Petrova

Spasova-Apostolova

et al. 2022

Plants

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In this research, the biosynthetic and biocontrol potential of endophytic yeast to improve the growth and development of tobacco has been elucidated. Three yeast strains were enriched and isolated from different plant tissues. Partial sequence analysis of ITS5-5.8-ITS4 region of the nuclear ribosomal DNA with universal primers identified YD5, YE1, and YSW1 as Saccharomyces cerevisiae (S. cerevisiae), Zygosaccharomyces bailii (Z. bailii), and Saccharomyces kudriavzevii (S. kudriavzevii), respectively. When cultivated in a medium supplemented with 0.1% L-tryptophan, isolated yeast strains produced indole-3-acetic acid (IAA). The capacities of those strains to improve the mobility of phosphorus and synthesize siderophores has been proven. Their antimicrobial activities against several Solanaceae plant pathogenic fungi (Alternaria solani pathovar. tobacco, Rhizoctonia solani, and Fusarium solani pathovar. phaseoli) were determined. S. cerevisiae YD5, Z. bailii YE1, and S. kudriavzevii YSW1 inhibited the growth of all tested pathogens. Yeast strains were tested for endophytic colonization of tobacco by two different inoculation methods: soil drench (SD) and leaf spraying (LS). To establish colonization in the various tissues of tested tobacco (Nicotiana tabaccum L.) plants, samples were taken on the seventh, fourteenth, and twenty-first days after treatment (DAT), and explants were inoculated on yeast malt agar (YMA). Both techniques of inoculation showed a high frequency of colonization from 83.33% to 100%. To determine the effectiveness of the microbial endophytes, their effect on some physiological processes in the plant were analyzed, such as photosynthesis, stomatal conductivity, and transpiration intensity. The effect of single and double treatment with yeast inoculum on the development and biochemical parameters of tobacco was reported. Plants have the ability of structural and functional adaptation to stress effects of different natures. All treated plants had a higher content of photosynthetic pigments compared to the control. Photosynthesis is probably more intense, and growth stimulation has been observed. The chlorophyll a/b ratio remained similar, and the total chlorophyll/carotenoid ratio slightly increased as a result of elevated chlorophyll levels. The most significant stimulating effect was recorded in tobacco plants treated by foliar spraying with Z. bailii YE1 and S. cerevisiae YD5. In contrast, S. kudriavzevii YSW1 had a better effect when applied as a soil drench. Thus, S. cerevisiae YD5, Z. bailii YE1, and S. kudriavzevii YSW1 have a high potential to be used as a biocontrol agents in organic agriculture.

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Section: Introductionmentioning

confidence: 99%

Tobacco Plant Growth-Promoting and Antifungal Activities of Three Endophytic Yeast Strains

Petkova

Petrova

Spasova-Apostolova

et al. 2022

Plants

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“…Endophytic yeasts have been associated with several plant species: Saccharomyces cerevisiae , considered endophytic of Ficus carica ( Ling et al., 2020 ); and Debaryomyces hansenii , endophytic of wheat ( Wachowska et al., 2018 ) and rice ( Tantirungkij et al., 2015 ), all identified in the apoplast of T. cacao . Although endophytes may play a role in increasing growth, acquiring nutrients and strengthening tolerance to stress in plants ( Ling et al., 2020 ), additional studies are needed. Such studies would offer a more comprehensive understanding of the possible symbiotic relationship between these microorganisms and T. cacao , clarifying the benefits for the host plant.…”

Section: Discussionmentioning

confidence: 99%

Apoplastomes of contrasting cacao genotypes to witches’ broom disease reveals differential accumulation of PR proteins

De Oliveira,

Alves,

Ferreira

et al. 2024

Front. Plant Sci.

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Witches’ broom disease (WBD) affects cocoa trees (Theobroma cacao L.) and is caused by the fungus Moniliophthora perniciosa that grows in the apoplast in its biotrophic phase and later progresses into the tissues, causing serious losses in the production of cocoa beans. Therefore, the apoplast of T. cacao can provide important defense responses during the interaction with M. perniciosa. In this work, the protein profile of the apoplast of the T. cacao genotypes Catongo, susceptible to WBD, and CCN-51, resistant one, was evaluated. The leaves of T. cacao were collected from asymptomatic plants grown in a greenhouse (GH) and from green witches’ brooms grown under field (FD) conditions for extraction of apoplastic washing fluid (AWF). AWF was used in proteomic and enzymatic analysis. A total of 14 proteins were identified in Catongo GH and six in Catongo FD, with two proteins being common, one up-accumulated, and one down-accumulated. In CCN-51, 19 proteins were identified in the GH condition and 13 in FD, with seven proteins being common, one up-accumulated, and six down-accumulated. Most proteins are related to defense and stress in both genotypes, with emphasis on pathogenesis-related proteins (PR): PR-2 (β-1,3-glucanases), PR-3 and PR-4 (chitinases), PR-5 (thaumatine), PR-9 (peroxidases), and PR-14 (lipid transfer proteins). Furthermore, proteins from microorganisms were detected in the AWF. The enzymatic activities of PR-3 showed a significant increase (p < 0.05) in Catongo GH and PR-2 activity (p < 0.01) in CCN-51 FD. The protein profile of the T. cacao apoplastome offers insight into the defense dynamics that occur in the interaction with the fungus M. perniciosa and offers new insights in exploring future WBD control strategies.

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“…Another factor that could interfere in diversity is the mutualistic relationship between microorganisms and plants. The characteristics of the type of colonized tissue can act by selecting the species that would colonize there (Ren et al 2016;Dhayanithy et al 2019;Ling et al 2020). Therefore, we believe that the nutritional characteristics of fruits (quality and availability of nutrients) may be the main factor influencing the richness and abundance of culturable fruit yeast population.…”

Section: Yeasts In Cerrado Fruitsmentioning

confidence: 96%

Yeasts in native fruits from Brazilian neotropical savannah: occurrence, diversity and enzymatic potential

et al. 2021

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Cerrado is the second largest phytogeographic domain in Brazil, with a huge ethnobotany variety, including fruit species that stand out for their economic, industrial, biotechnological and medicinal potential. The objective of this study was to characterize the diversity of culturable yeasts and their potential for the production of hydrolytic enzymes in fruits of 13 species of native plants of the Cerrado in Brazil. Sequencing the 26S rRNA gene identified the isolates. The enzymatic potential was evaluated using specific substrates for the enzymes amylases, cellulases, proteases, and pectinases. Nine of the 13 fruit species analyzed showed yeast growth, totaling 82 isolates, identified in 26 species. The phylum Ascomycota predominated over Basidiomycota. The fruits of Butia capitata presented the highest species richness. Candida and Meyerozyma were the most frequent genera. About 57% of the isolates were able to produce at least one of the enzymes analyzed. The species Papiliotrema flavescens, Hanseniaspora meyeri, Meyerozyma guilliermondii, and Rhodotorula mucilaginosa produced all the enzymes tested. The results were found to expand the knowledge about the yeast communities present in fruits of the Cerrado native plants, evidencing the presence of species shared among the plants, and their potential for biotechnological use in the future.

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A potentially important resource: endophytic yeasts

Cited by 19 publications

References 36 publications

Tobacco Plant Growth-Promoting and Antifungal Activities of Three Endophytic Yeast Strains

Tobacco Plant Growth-Promoting and Antifungal Activities of Three Endophytic Yeast Strains

Apoplastomes of contrasting cacao genotypes to witches’ broom disease reveals differential accumulation of PR proteins

Yeasts in native fruits from Brazilian neotropical savannah: occurrence, diversity and enzymatic potential

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