A potentiometric biosensor for rapid on-site disease diagnostics

Abstract

Quantitative point-of-care (POC) devices are the next generation for serological disease diagnosis. Whilst pathogen serology is typically performed by centralized laboratories using Enzyme-Linked ImmunoSorbent Assay (ELISA), faster on-site diagnosis would infer improved disease management and treatment decisions. Using the model pathogen Bovine Herpes Virus-1 (BHV-1) this study employs an extended-gate field-effect transistor (FET) for direct potentiometric serological diagnosis. BHV-1 is a major viral pathogen of Bovine Respiratory Disease (BRD), the leading cause of economic loss ($2 billion annually in the US only) to the cattle and dairy industry. To demonstrate the sensor capabilities as a diagnostic tool, BHV-1 viral protein gE was expressed and immobilized on the sensor surface to serve as a capture antigen for a BHV-1-specific antibody (anti-gE), produced in cattle in response to viral infection. The gE-coated immunosensor was shown to be highly sensitive and selective to anti-gE present in commercially available anti-BHV-1 antiserum and in real serum samples from cattle with results being in excellent agreement with Surface Plasmon Resonance (SPR) and ELISA. The FET sensor is significantly faster than ELISA (<10 min), a crucial factor for successful disease intervention. This sensor technology is versatile, amenable to multiplexing, easily integrated to POC devices, and has the potential to impact a wide range of human and animal diseases.

The application of graphene for in vitro and in vivo electrochemical biosensing

“…The proposed sensor showed enhanced analytical performance toward Na þ ions detection (lower limit of detection and better selectivity) comparatively to previously reported devices. In a recently published work, Tarasov et al, (2016) proposed a disposable potentiometric biosensor for direct serological diagnosis. In this study, the viral pathogen Bovine Herpes Virus-1 (BHV-1) was determined.…”

Biotechnological innovations in farm and pet animal disease diagnosis

“…Biosensors work on various principles viz detecting the changes in the pH, the ion concentrations, mass by specific hybridization, enzymatic reaction, loss of functionality, change in the electrical potential, change in color, and temperature. Based on these principles, many biosensors have been devised for the detection of animal pathogens; for example, an extended-gate field-effect transistor for the direct potentiometric serological diagnosis of the BHV-1 (Tarasov et al, 2016), nanowire-based immunosensor for bovine viral diarrhea virus (BVDV) (Montrose et al, 2015), luminescence resonance energy transferÀbased biosensors for the ultrasensitive detection of the H7 strain (Ye et al, 2014b), quartz crystal microbalance (QCM)Àbased immunosensors to detect H5N1 (Li et al, 2011), and SpectroSensTM optical microchip sensors for foot-and-mouth disease virus (FMDV) (Bhatta et al, 2012).…”

Recent Advancement in Biosensors Technology for Animal and Livestock Health Management

“…The biosensor was presented to be sensitive and selective to anti-IgE present in commercially available anti-BHV-1 antiserum and in real serum samples from cattle. The system was shown to be faster than the traditionally used ELISA, amenable to multiplexing, and easily integrated into POC devices (Tarasov et al, 2016).…”