The neonatal stage in the first month of life; the neonatal period is the first 4 week of a Childs life. Infections of neonatal during neonatal period are called as neonatal infections. Neonatal infections are the most common cause of neonatal mortality. The present aim of the study was to access the knowledge regarding neonatal infection among postnatal mothers who were admitted in the postnatal ward at saveetha medical college and hospital. A quantitative approach with descriptive research design was adopted for the present study. 60 postnatal mother among which (n = 60) were selected by using non-probability purposive sampling technique. A self -structured method questionnaire was used to collect both the demographic data and the existing level of knowledge on neonatal infection among postnatal mothers. Among 60 study participants, the mean scene on existing level of knowledge on neonatal infection among postnatal mothers was found to be 6.13 with standard deviation 2.07 hence the findings of present study conducted that, postnatal mothers had an inadequate level of knowledge on neonatal infection.