A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis

Kumar, Vinod; Sood, Shudhanta; Vasudevan, Karthikeyan; Umapathy, Govindhaswamy

doi:10.1016/j.mex.2021.101578

Cited by 5 publications

(10 citation statements)

References 16 publications

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“…Faecal progesterone metabolites were quantified using previously developed EIA [31] , [32] , [33] , [34] . In progesterone EIA, monoclonal antibody (CL425, provided by Dr. Coralie Munro, University of California, USA) was diluted to 1:6000, HRP (Horseradish peroxidase) conjugated progesterone diluted to 1:100,000 and progesterone standards (200 pg/well to 0.39 pg/well).…”

Section: Methodsmentioning

confidence: 99%

Development of an enzyme immunoassay to measure urinary and faecal 5α-androst-16-en-3-one in pigs

Kumar¹,

Umapathy²

2023

MethodsX

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Section: Methodsmentioning

confidence: 99%

Development of an enzyme immunoassay to measure urinary and faecal 5α-androst-16-en-3-one in pigs

Kumar¹,

Umapathy²

2023

MethodsX

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“…Fecal progestogens, estrogens, and androgens were measured using the previously described methods [ 49 , 50 , 51 , 52 ]. Antibodies and HRP conjugates for progesterone (Monoclonal progesterone, Quidel clone no.…”

Section: Methodsmentioning

confidence: 99%

“…The testosterone antibody was diluted to 1:10,000 and 1:200,000 for HRP conjugated testosterone and standards (600-1.17 pg/well). Crossreactivity of testosterone was reported previously [49][50][51]. The procedure for EIAs was performed as previously described [46][47][48].…”

Section: Eia For Progesterone Estradiol and Testosteronementioning

confidence: 99%

Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)

Kumar

Manu

Caroline

et al. 2022

Cells

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We discovered odorous 16-androstenes (Androstenone and Androstenol) in endangered mouse deer during a captive breeding program. This study examined the molecular characteristics, their synthesis pathway, and the possible functional role of these compounds in the reproduction of mouse deer. CYP17A1 and CYB5 genes were cloned and expressed in HEK-293, COS-7 cell lines, and gonads of mouse deer to investigate the CYP17A1 gene’s andien-β-synthase activity towards the synthesis of 16-androstenes in mouse deer. An enzyme immunoassay was further developed and standardized to measure fecal androstenone during the reproductive cycles of mouse deer. Results showed that the mouse deer CYP17A1 gene possesses andien-β-synthase activity and could transform pregnenolone into 5,16-androstadien-3β-ol. The expression of the CYP17A1 gene upregulated in the testis and ovary compared to other tissues in mouse deer. Significantly elevated androstenone and estrogens were recorded prior to delivery and postpartum estrus/mating in mouse deer. Further, there were weak correlations between fecal androstenone and estrogens/androgens in mouse deer during the breeding season. These findings suggest that androstenone probably plays a role in the reproductive activities of mouse deer. This knowledge can be used for captive breeding programs of mouse deer in India and elsewhere.

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“…The testosterone antibody was diluted to 1:10,000 and 1:2,00,000 for HRP conjugated testosterone and standards (600-1.17 pg/well). Cross-reactivity of testosterone is reported previously (44)(45)(46). The procedure for EIAs was performed as previously described (44)(45)(46).…”

Section: Eia For Progesterone Estradiol and Testosteronementioning

confidence: 99%

“…Cross-reactivity of testosterone is reported previously (44)(45)(46). The procedure for EIAs was performed as previously described (44)(45)(46).…”

Section: Eia For Progesterone Estradiol and Testosteronementioning

confidence: 99%

Discovery of Boar Pheromones and their Functional Role in Reproduction of Mouse Deer (<em>Moschiola Indica</em>)

Kumar¹,

Manu²,

Caroline³

et al. 2022

Preprint

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Two putative boar pheromones (Androstenone and Androstenol) were discovered in endangered mouse deer during captive breeding program. This study further examined the molecular characteristics, pheromone synthesis pathway, and the functional role of these pheromones in reproduction of mouse deer. CYP17A1 and CYB5 genes were cloned and expressed in HEK-293, COS-7 cell lines and gonads of mouse deer to investigate CYP17A1 gene’s andien-β-synthase activity towards synthesis of sex pheromones in mouse deer. An enzyme immunoassay was also developed and standardized to measure the fecal androstenone during reproductive cycles of mouse deer. Results showed that mouse deer’s CYP17A1 gene possesses andien-β-synthase activity and could transform pregnenolone into 5,16-androstadien-3β-ol. The expression of CYP17A1 gene upregulated in the testis and ovary, compared to other tissues in mouse deer. Significantly elevated pheromones and estrogens were recorded prior to delivery and postpartum estrus / mating in mouse deer. Further, there were weak correlations between fecal pheromones and estrogens/ androgens in mouse deer during breeding season. The findings suggest that the boar pheromones might play a direct role in the reproductive activities of mouse deer which might be used for breeding of mouse deer elsewhere.

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A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis

Cited by 5 publications

References 16 publications

Development of an enzyme immunoassay to measure urinary and faecal 5α-androst-16-en-3-one in pigs

Development of an enzyme immunoassay to measure urinary and faecal 5α-androst-16-en-3-one in pigs

Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)

Discovery of Boar Pheromones and their Functional Role in Reproduction of Mouse Deer (<em>Moschiola Indica</em>)

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