A practical protocol to prepare paraffin-embedded whole tick histology sections

Yang, Tzushan S.; LaDouceur, Elise E B; Baumgartner, Wes; Marr, Henry S.; Karounos, Michael; Robertson, James; Whitehurst, Nathan; Miller, Laura S.; Birkenheuer, Adam J.

doi:10.1016/j.ttbdis.2023.102162

Cited by 1 publication

(2 citation statements)

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“…Routine histological preparation of a whole tick is challenging due to the tick’s chitinous exoskeleton, which is rigid and impermeable. 26 Specialized techniques and equipment, not always available in laboratories, are more suitable for a detailed evaluation of the tick’s histological structures. 26 A recent study demonstrated that Bouin’s solution is more effective than 10% neutral-buffered formalin for tick fixation, allowing for the generation of sections with more histological details of the parasite’s internal structures.…”

Section: Discussionmentioning

confidence: 99%

“…26 Specialized techniques and equipment, not always available in laboratories, are more suitable for a detailed evaluation of the tick’s histological structures. 26 A recent study demonstrated that Bouin’s solution is more effective than 10% neutral-buffered formalin for tick fixation, allowing for the generation of sections with more histological details of the parasite’s internal structures. 26 However, in the cases evaluated in this study, tick fixation was performed with 10% neutral-buffered formalin, which probably interfered with obtaining sections of the whole tick.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of cutaneous lesions caused by natural tick infestation in cattle

Melo,

Santos,

Almeida

et al. 2023

Vet Pathol

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Although tick infestation is a significant health problem in livestock, there are limited studies on the dermatopathological aspects of natural tick infestation in cattle. This study aimed to describe the gross and histologic aspects of cutaneous lesions caused by tick infestation in cattle. Thirteen cases were selected based on necropsy data from a 10-year retrospective study. Predispositions were observed in beef cattle ( P = .049) and the Angus breed ( P = .012), and lesions occurred mainly in the fall ( P = .007). Gross lesions included hypotrichosis (13/13; 100%), scales (12/13; 92%), alopecia (11/13; 85%), ulcers (7/13; 54%), crusts (7/13; 54%), and erosions (2/13; 15%). These gross lesions were mainly located in the thorax (12/13; 92%), head (11/13; 85%), abdomen (10/13; 77%), neck (9/13; 69%), limbs (9/13; 69%), and perineum (9/13; 69%). Histologically, all cases had ticks adhered to the epidermis with erosions (13/13; 100%), ulcers (11/13; 85%), orthokeratotic hyperkeratosis (13/13; 100%), irregular acanthosis (13/13; 100%), intraepidermal pustules (13/13; 100%), crusts (10/13; 77%), and ballooning degeneration (4/13; 31%). In the dermis, just below the tick insertion site, there was coagulation necrosis, fibrin deposition, and inflammatory infiltrate composed of mixed cells (neutrophils, lymphocytes, plasma cells, macrophages, and few eosinophils) (9/13; 69%), neutrophils (3/13; 23%), or eosinophils (1/13; 8%). This study reinforces the different patterns of cutaneous lesions caused by tick infestation in cattle, which should be considered as a potential cause of dermatitis in this species.

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Section: Discussionmentioning

confidence: 99%