A practical strategy to develop isoform-selective near-infrared fluorescent probes for human cytochrome P450 enzymes

Real-time fluorescent monitoring of phase I xenobiotic-metabolizing enzymes

“…The potential activity of fluorescent probe 2 in detecting endogenous substances was demonstrated in HeLa and HepG2 cells, with a fluorescence emission spectrum observed in the range of 690–750 nm. 41…”

“…The potential activity of uorescent probe 2 in detecting endogenous substances was demonstrated in HeLa and HepG2 cells, with a uorescence emission spectrum observed in the range of 690-750 nm. 41 Another NIR uorescent probe 3 was designed for real-time tracking of CYP2J2 in complex biological systems, leveraging the overexpression of CYP2J2 in various cancer cells. The basic uorophore component, (E)-2-(2-(6-hydroxy-2,3-dihydro-1Hxanthen-4-yl) vinyl)-3,3-dimethyl-1-propyl-3H-indol-1-ium iodide (HXPI), was selected for its long aromatic structure, photoemissive properties, and high tissue penetration.…”

Fluorescence-Based High-Throughput Assays for Investigating Cytochrome P450 Enzyme-Mediated Drug–Drug Interactions

“…For example, DBF, a multi-enzyme metabolizing substrate, was identified as a useful tool in HTS assay for CYP3A7 to realize the DDI/HDIs for neonatal population (Work et al, 2021). It is noteworthy that part of the fluorogenic (Li et al, 1997;Renwick et al, 2001;Dai et al, 2017;Ning et al, 2018b;Ning et al, 2019a;Ning et al, 2019c;Jin et al, 2020;Dai et al, 2021;Feng et al, 2022;Tian et al, 2022;He et al, 2023).…”

Benzorhodol derived far-red/near-infrared fluorescent probes for selective and sensitive detection of butyrylcholinesterase activity in living cells and the non-alcoholic fatty liver of zebrafish

“…[19][20][21][22] Such a method requires the addition of extra reagents, making it unsuitable for intracellular or in vivo assays. In contrast, far-red/near-infrared (FR/NIR) uorescence probing, as a kind of non-invasive bio-imaging strategy, demonstrates versatility and high reliability in detecting biological enzymes in living systems, [23][24][25][26] making it a promising alternative. In 2017, Yang et al identied cyclopropanecarboxylate as the specic substrate of BChE.…”