2016
DOI: 10.1371/journal.ppat.1006091
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A Precise Temperature-Responsive Bistable Switch Controlling Yersinia Virulence

Abstract: Different biomolecules have been identified in bacterial pathogens that sense changes in temperature and trigger expression of virulence programs upon host entry. However, the dynamics and quantitative outcome of this response in individual cells of a population, and how this influences pathogenicity are unknown. Here, we address these questions using a thermosensing virulence regulator of an intestinal pathogen (RovA of Yersinia pseudotuberculosis) as a model. We reveal that this regulator is part of a novel … Show more

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Cited by 31 publications
(23 citation statements)
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“…RovA was involved in these changes, and is known to positively regulate invasin and pH6 antigen expression. RovA expression is bistable in bacteriological media between 30 and 34°C, indicating bacteria utilize a bet-hedging approach to prepare a subset of the population to invade host cells, potentially as bacteria are moving through the host environment and the temperature is increasing (Quade et al, 2012 ; Nuss et al, 2016 ). RovA expression was also detected within a subset of the bacterial population replicating within the cecum, suggesting that RovA expression could be another example of cooperative behavior (Nuss et al, 2016 ).…”
Section: Yersinia Human Disease Progressionmentioning
confidence: 99%
“…RovA was involved in these changes, and is known to positively regulate invasin and pH6 antigen expression. RovA expression is bistable in bacteriological media between 30 and 34°C, indicating bacteria utilize a bet-hedging approach to prepare a subset of the population to invade host cells, potentially as bacteria are moving through the host environment and the temperature is increasing (Quade et al, 2012 ; Nuss et al, 2016 ). RovA expression was also detected within a subset of the bacterial population replicating within the cecum, suggesting that RovA expression could be another example of cooperative behavior (Nuss et al, 2016 ).…”
Section: Yersinia Human Disease Progressionmentioning
confidence: 99%
“…pseudotuberculosis YPIII ( mRuby2 ) or YPIII Δ cnfY ( mRuby2 ) were sacrificed by CO 2 asphyxiation at day 3 or day 42 post infection. The cecum was isolated and cryosections of the ceca were prepared for fluorescent microscopy as described [ 81 ]. The cryo-sections were examined with the Axiovert II fluorescence microscope (Zeiss) using the Axiocam HR digital CCD camera (Zeiss) and the software ZEN 2012 (Zeiss).…”
Section: Methodsmentioning
confidence: 99%
“…At an increased temperature (>42°C), the coiled‐coil motif unfolds and TlpA is unable to bind DNA and repress gene expression. Additional examples of proteinaceous thermosensors have since then been identified in other pathogens such as the transcriptional regulator RovA in Yersinia which positively regulates expression of itself and the gene encoding Invasin, an outer membrane protein binding β1‐integrins in the host (Herbst et al, ; Nuss et al, ; Revell & Miller, ). GmaR in Listeria monocytogenes acts as an anti‐repressor, binding to the transcriptional repressor MogR (Kamp & Higgins, ).…”
Section: Discovering How Bacteria Feel the Heatmentioning
confidence: 99%