A precisely targeted application strategy of dipping young cucumber fruit in fungicide to control cucumber gray mold

He, Lifei; Cui, Kaidi; Song, Yufei; Zhang, Zhengqun; Li, Beixing; Mu, Wei; Liu, Feng

doi:10.1002/ps.5055

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…The toxicities of 5% ME emamectin benzoate, 1.8% ME abamectin, 5% EC fluopyram, and 5% EC cyclobutrifluram against B. xylophilus were assessed using the dipping method [26]. These agents were prepared in at least six mass concentration gradients (Table S1), with one treatment for each concentration and distilled water serving as the control.…”

Section: Toxicity Test Against B Xylophilusmentioning

confidence: 99%

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Bursaphelenchus xylophilus

Liu,

Shao,

et al. 2024

IJMS

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Bursaphelenchus xylophilus is a dangerous quarantine pest that causes extensive damage to pine ecosystems worldwide. Cyclobutrifluram, a succinate dehydrogenase inhibitor (SDHI), is a novel nematicide introduced by Syngenta in 2013. However, the nematocidal effect of cyclobutrifluram against plant-parasitic nematodes remains underexplored. Therefore, here, we aim to address this knowledge gap by evaluating the toxicity, effects, and mode of action of cyclobutrifluram on B. xylophilus. The result shows that cyclobutrifluram is the most effective agent, with an LC50 value of 0.1078 mg·L−1. At an LC20 dose, it significantly reduced the population size to 10.40 × 103 ± 737.56—approximately 1/23 that of the control group. This notable impact may stem from the agent’s ability to diminish egg-laying and hatching rates, as well as to impede the nematodes’ development. In addition, it has also performed well in the prevention of pine wilt disease, significantly reducing the incidence in greenhouses and in the field. SDH consists of a transmembrane assembly composed of four protein subunits (SDHA to SDHD). Four sdh genes were characterized and proved by RNAi to regulate the spawning capacity, locomotion ability, and body size of B. xylophilus. The mortality of nematodes treated with sdhc-dsRNA significantly decreased upon cyclobutrifluram application. Molecular docking further confirmed that SDHC, a cytochrome-binding protein, is the target. In conclusion, cyclobutrifluram has a good potential for trunk injection against B. xylophilus. This study provides valuable information for the screening and application of effective agents in controlling and preventing PWD in forests.

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Section: Toxicity Test Against B Xylophilusmentioning

confidence: 99%

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Bursaphelenchus xylophilus

Liu,

Shao,

et al. 2024

IJMS

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Effect of Ginkgo biloba endophytic bacterium Variovorax sp. R1 on the biological control of postharvest cucumber gray mold and related physiological mechanisms

Yang

Ding

et al. 2023

Eur J Plant Pathol

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Development of Boscalid Resistance in Botrytis cinerea and an Efficient Strategy for Resistance Management

Cui

et al. 2021

Plant Disease

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Among succinate dehydrogenase inhibitors (SDHI), only boscalid has been registered in China for controlling gray mold. In Shandong Province of China, it has been more than a decade since the first use of boscalid to control gray mold. In the current study, we monitored the resistance development process of Botrytis cinerea to boscalid, identified the mutation types that occurred in boscalid-resistant isolates, and proposed an original application technique to delay resistance development. A total of 720 B. cinerea isolates collected from tomato and cucumber in Shandong Province from 2014 to 2019 were determined to be sensitive to boscalid. The results showed that the sensitivity of the B. cinerea isolates to boscalid declined gradually over time, with a mean EC50 value of 0.3 ± 0.02 mg L−1 in 2014 and 6.39 ± 1.66 mg L−1 in 2019. The proportion of resistant isolates quickly increased from 0.81% in 2014 to 28.97% in 2019. Mutations of P225F, N230I, H272Y and H272R in the SdhB subunit were responsible for boscalid resistance. Four concurrent mutations (G85A, I93V, M158V and V168I) in the SdhC subunit were first discovered in Shandong Province, but they did not affect the level of boscalid resistance. Interestingly, this study found that the dipping fruit application, a precise topical application technique, could delay the development of boscalid resistance. Therefore, this application technique provides a new way for the resistance management of B. cinerea.

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A precisely targeted application strategy of dipping young cucumber fruit in fungicide to control cucumber gray mold

Cited by 4 publications

References 29 publications

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Bursaphelenchus xylophilus

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Bursaphelenchus xylophilus

Effect of Ginkgo biloba endophytic bacterium Variovorax sp. R1 on the biological control of postharvest cucumber gray mold and related physiological mechanisms

Development of Boscalid Resistance in Botrytis cinerea and an Efficient Strategy for Resistance Management

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