A Precision Hand Trimmer for Electron Microscope Tissue Blocks

Butler, James K.

doi:10.3109/10520297409116962

Cited by 9 publications

(3 citation statements)

References 2 publications

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“…To this author's knowledge, only one such block holder is commercially available (Butler, 1974), although others have been described in the literature (Boyd, 1960;Danon, 1961). The Butler Block Trimmer is currently offered by most EM supply houses for approximately $300 USD.…”

Section: Introductionmentioning

confidence: 99%

Inexpensive holder for hand‐trimming resin‐embedded tissues

Wise

2002

Microscopy Res & Technique

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A simple and inexpensive mount for securely holding resin-embedded tissues for trimming prior to sectioning for light or electron microscopy is described. The unit is composed of a base plate from which a 12.7-mm drill chuck protrudes. A tissue block is clamped into a microtome chuck and that is mounted in the drill chuck. A standard dissecting microscope is placed over the unit and used to magnify the block for trimming. Total cost of materials is estimated to be $20-40 USD.

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Section: Introductionmentioning

confidence: 99%

Inexpensive holder for hand‐trimming resin‐embedded tissues

Wise

2002

Microscopy Res & Technique

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“…In comparison to hand trimmers which simply employ knife guides (Butler 1974, Danon 1961, Guglielmotti 1976, Schneider and Sasaki 1976, the trimmer described in the present communication allows more accurate, mechanically-guided trimming from untrimmed plastic specimen blocks. The device, in conjunction with an ultramicrotome and a stereomicroscope, facilitates rapid, preferential shaping of the block face to leave only the area of interest at.…”

mentioning

confidence: 99%

“…Several devices and techniques have been described which aid in the trimming of plastic embedded specimen blocks to form "pyramids" (= truncated pyramids) before they are mounted into the microtome (Boyde 1960, Butler 1974, Danon 1961, Guglielmotti 1976, Schneider and Sasaki 1976, Sjostrand 1967. Gorycki (1978) described a "rectangular trimming block" which, in conjunction with a small mirror mounted on the knife holder of the ultramicrotome, squarely retrimmed the block face of a preformed truncated pyramid.…”

mentioning

confidence: 99%