A Preclinical Study of the Effects of Seabuckthorn (Hippophae rhamnoides L.) Leaf Extract on Cutaneous Wound Healing in Albino Rats

Abstract

Hippophae rhamnoides L. (family Elaeagnaceae), commonly known as seabuckthorn, is a wild shrub growing at high altitude (1200-4500 meters) in adverse climatic conditions. The aim of the present study was to evaluate healing potential of seabuckthorn leaves in a preclinical study on rats using a cutaneous excision-punch wound model. Four full-thickness excision-type wounds of 8.0 mm diameter were created on the dorsal surface of rats under aseptic conditions. The aqueous lyophilized extract of seabuckthorn leaves, at doses of 0.5%, 1.0%, and 1.5% w/v prepared in propylene glycol, were applied topically twice daily for 7 days. Control animals received the vehicle alone in an identical manner. Wound granulation tissues were excised on eighth day postwounding, and the hydroxyproline, hexosamine, total protein content, and antioxidant levels were determined. Wound surface area was also measured on the eighth day before wound excision to determine wound contraction. Topical application of 1.0% seabuckthorn leaf extract statistically significantly augmented the healing process, as evidenced by increases in the content of hydroxyproline and protein as well as the reduction in wound area when compared with similar effects in response to treatment using povidone-iodine ointment (standard care). The reduced glutathione, vitamin C, superoxide dismutase, catalase, and glutathione peroxidase activities showed significant increases in seabuckthorn leaf extract-treated wounds as compared to controls. The lipid peroxide levels were significantly decreased in leaf extract-treated wounds. The results suggest that aqueous leaf extract of seabuckthorn promotes wound healing, which may be due to increased antioxidant levels in the granulation tissue.

Wound healing potential of Capparis spinosa against cutaneous wounds infected by Escherichia coli in a rat model

“…Chithra et al [24] and Pather et al [25] showed that collagen content reached its peak on day 8 and then a mild continuous decline occurred until day 16 [24,25]. In 2005, Gupta et al [26] demonstrated that topical application of 1% seabuckthorn leaf extract significantly improved the healing process, as evidenced by increase in the content of hydroxyproline and protein as well as the reduction in wound area on day 8 post wounding. In a study by Nayak et al [27] contents of protein and hydroxyproline were increased significantly in groups treated by Cecropia peltata, meaning that proliferative stage reached a peak on day 7 or 8 of post wounding [24,25].…”

Effects of Sea Buckthorn (Hippophae rhamnoides L.) Fruit Extract on Ultraviolet-induced Apoptosis of Skin Fibroblasts

“…Sea buckthorn의 다양한 부위는 피부질환, 위궤양, 천식, 폐질환 등을 치료하기 위해 오랫동안 사용되어 왔다 [34]. 특별히, sea buckthorn의 잎 추출물, 열매, 씨앗오일 등은 진피 상처치료와 다양한 요인에 의한 화상치료 등에도 우수한 효능 을 나타내었다 [15]. 그러나, Sea buckthorn의 잠재적인 치료효 과에 대한 이러한 보고에도 불구하고, 이들을 이용하여 UV에 의한 세포사와 관련된 작용기전에 대한 연구는 거의 알려져 있지 않다.…”

Effects of Alchemilla vulgaris and glycerine on epithelial and myofibroblast cell growth and cutaneous lesion healing in rats

“…The protocol employed was similar to that described by Gupta et al (2005). The back was shaved and three circular cutaneous excisional lesions were created on each side of the back by gently rotating a biopsy punch (a pencil-like metal tube with a cutting edge) under light ether anesthesia.…”

“…The aim of the present study was therefore twofold: (i) to study the putative effects of A. vulgaris on epithelial and myofibroblast cell growth in vitro, and (ii) to investigate whether A. vulgaris could affect wound healing in a conventional in vivo rat model of cutaneous excisional lesions (Dorsett-Martin, 2004;Gupta et al, 2005).…”