A preliminary comparative immunohistochemical, radioimmunological, and biological study of arginine vasotocin (AVT) in the pineal gland and urophysis of some teleostei

Holder, F.C.; Schroêder, M.; Guerné, J.M.; Vivien-Rœls, B.

doi:10.1016/0016-6480(79)90041-8

Cited by 20 publications

(2 citation statements)

References 33 publications

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“…The AVT concentrations in flounder’s ( Platichthys flesus ), silver eel’s ( Anguilla anguilla ), long-spined bullhead’s ( Cottus bubalis ) and five-bearded rockling’s ( Onos mustela ) urophysis ranged from 0.096 to 0.276 pmol/gland (Holden et al 1979; Harding et al 1997). …”

Section: Resultsmentioning

confidence: 99%

“…It is well known that urophysis is a site of storage and release of peptides, that is, urotensin I (UI), urotensin II (UII), corticotropin-releasing factor (CRF) and parathyroid hormone-related protein (PTHrP) (Pearson et al 1980; Lederis et al 1982; Ingleton et al 2002), which are synthesized by Dahlgren cells. Also, AVT-like immunoreactivity has been reported in urophysis of several fish species (Holden et al 1979; Harding et al 1997). To the authors’ knowledge, IT has not been yet identified here.…”

Section: Introductionmentioning

confidence: 99%

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Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species

Gozdowska

Ślebioda²,

Kulczykowska

2012

Fish Physiol Biochem

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In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish species. We have used high-performance liquid chromatographic assay with fluorescence detection (HPLC-FL) preceded by solid-phase extraction (SPE) and liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI MS/MS) technique to determine both neuropeptides in urophysis of three fish species. The efficiency of peptide’s SPE extraction was 79–85 %. In HPLC-FL method, the limits of detection (LOD) and quantification (LOQ) were estimated as 1.0 and 3.4 pmol/mL for IT and 0.25 and 2.20 pmol/mL for AVT. In LC–MS/MS method, LOD and LOQ were estimated as 0.4 and 1.2 pmol/mL for IT and 0.06 and 0.2 pmol/mL for AVT. The chromatographic methods are good alternative for RIA, because enable to measure both nonapeptides simultaneously in one sample. In round goby (Neogobius melanostomus), three-spined stickleback (Gasterosteus aculeatus) and sea bream (Sparus aurata), urophysial IT concentrations ranged between 0.056 and 0.678 pmol/mg tissue and AVT concentrations ranged between 0.0008 (or even below detection threshold) and 0.084 pmol/mg tissue.

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Section: Resultsmentioning

confidence: 99%