A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED

Abstract

The aim of this preliminary study was to compare the effects of different energy densities from red and infrared low-level laser (LLL) on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). SHED were irradiated with red laser (R) or infrared laser (IR) set with the following dosimetry: 1.2 J/cm (0.05 J), 2.5 J/cm (0.1 J), 5.0 J/cm (0.2 J), and 7.5 J/cm (0.3 J). Positive (C+) and negative (C-) control groups comprised non-irradiated cells. Data were analyzed by two-way ANOVA followed by Tukey's test (P < 0.05). At 24- and 48-h period, group R5.0 showed significantly higher cell viability rates than R1.2 and R2.5. At 48 h, R2.5 also revealed lower proliferation than R5.0. Comparing to the C+ group, R2.5 exhibited lower viability at 72 h, and proliferation at 24 and 48 h. Groups R1.2, IR1.2, and IR5.0 were less viable at 24 h, while R1.2, IR2.5, and IR5.0 revealed lower proliferative capacity at 48 h. Overall, our results showed that LLL can favor viability and proliferation of SHED, especially when cells receive red laser irradiation at 5.0 J/cm. Therefore, according to this preliminary investigation, 5 J/cm applied by red LLL induced high rates of cell viability and proliferation, while the same irradiation dose using infrared laser led to negative effects. LLL irradiation with 1.2 and 2.5 J/cm was deleterious to metabolic activity and proliferation of SHED regardless of the type of laser. Further studies are necessary to gain in-depth knowledge about the effects of different wavelengths of LLL on SHED viability and proliferation.

Newly synthesized series of oxoindole–oxadiazole conjugates as potential anti-SARS-CoV-2 agents:in silicoandin vitrostudies

“…Then, the crystal violet dye per well was dissolved using absolute CH 3 OH (100 ml) to measure the optical density of the produced color using an Anthos Zenyth 200rt plate reader at 570 nm. 55 4.2.3. SARS-CoV-2 main protease inhibitory activity.…”

β-Blockers bearing hydroxyethylamine and hydroxyethylene as potential SARS-CoV-2 Mpro inhibitors: rational based design,in silico,in vitro, and SAR studies for lead optimization

“…Absolute CH 3 OH (100 μl) was added to dissolve the crystal violet dye per well to measure the optical density of the produced color using Anthos Zenyth 200rt plate reader at 570 nm. 60 The IC 50 value for each tested compound which is corresponding to its minimum concentration required to reduce the virus infectivity by 50% in comparison to the virus control was calculated.…”

Photobiomodulation Effect of Different Diode Wavelengths on the Proliferation of Human Gingival Fibroblast Cells

“…The end power of each laser was checked by Power Meter (Laserpoint, Italy) prior to irradiation. Cell proliferation was assessed by MTT conducted at 24, 72 and 120 h following PBM irradiation (28).…”

“…In general, parameter standardization in PBM is necessary in order to improve the effects of this technology (27). This study was motivated by the fact the number of studies focused on the comparison of various energy densities and wavelengths of lasers in PBM treatments is limited (7,(26)(27)(28). Thus, this study was focused on comparing the PBM effects of various energy densities and wavelengths of diode lasers on the proliferation of human gingival fibroblast cells.…”