A preliminary linkage map of the tick, Ixodes scapularis

Abstract

Abstract.A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F 1 intercross progeny from a single, field-collected P 1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ∼10 9 bp, the relationship of physical to genetic distance is ∼300 kb/cM in the I. scapularis genome.