A preliminary map of genomic sites for F-attachment in Escherichiacoli Kl2

The pattern of evolutionary change in bacteria

“…These two sites may be relics of the integrated episome. (Matney, Goldschmidt, Erwin and Scroggs (1964) report the existence of at least four other pairs of * Condamine (1971) has presented evidence suggesting that the proA and proB genes are more closely linked than was previously supposed. He also reports the existence of revertible mutations which abolish the expression of both genes and suggests that they constitute an operon.…”

“…In E. coil, although sex factors with this sort of specificity are known (Kahn, 1968;Pearce and Meynell, 1968), other sex factors, e.g. F, transmit the chromosome with a variety of different gene orders (Matney, Goldschmidt, Erwin and Scroggs, 1964). In Pseudomonas matings a gene is thus always linked to all these genes that are transmitted before it, whilst in the Enterobacteriaceae there is no such regularity.…”

Coordination of a histidine residue of the protein‐component S to the cobalt atom in coenzyme B12‐dependent glutamate mutase from Clostridium cochlearium

“…For labelling of component S with lSN, the E. coli strain MC4100 containing pOZ3 was grown on M9 minimal medium containing (tSNHa)2SO4 (1 g/l). The his E. coli strain KI2 Hfr G6 [19] was grown in M9 medium containing ('5NH4)2SO 4 (1 g/l)and 1 mM [14N]histidine.…”

Regulation of hisHF Transcription of Aspergillus nidulans by Adenine and Amino Acid Limitation

“…The S. cerevisiae strain RH1616 (MAT␣,his7⌬ϻhis7 p -lacZ, gcn4-101) (Kuenzler et al, 1993) was used as recipient for a his7⌬ complementing cDNA of a galactose inducible A. nidulans expression library. The E. coli strains HfrG6 ( Ϫ , hisA323) (Matney et al, 1964), SB3931 ( Ϫ , hisF860), and UTH1767 ( Ϫ , hisH1767) (Goldschmidt et al, 1970) with stable mutations in hisA, hisF, and hisH, respectively, were obtained from the E. coli Genetic Stock Center (Yale University, New Haven, CT) and served as recipients for the cDNA found by the yeast complementation experiment. The A. nidulans strain GR5 (wA3, pyrG89, pyroA4, veA1) from G. May (Houston, TX) was recipient for the plasmids pME1565 and pME1608.…”

“…The plasmid DNA pME1611 from one of the transformants was retransformed into RH1616 to verify its ability to complement the histidine auxotrophy. Plasmid pME1611 was further analyzed by transforming it into the histidine auxotrophic E. coli strains HfrG6 (Matney et al, 1964) and UTH1767 and SB3931 (Goldschmidt et al, 1970) containing mutations in the hisA, hisH, and hisF genes, respectively. Although no functional E. coli promoter was present on the plasmid, the transformed DNA was able to complement the mutations in hisH and hisF, encoding a glutamine amidotransferase and a cyclase, respectively, but not in hisA, encoding imidazole-carboxamide isomerase.…”