A preliminary microsatellite genetic map of the ostrich (&lt;i&gt;Struthio camelus&lt;/i&gt;)

Huang, Yinhua; Liu, Q.; Tang, Bo; Lin, Li; Liu, W.; Zhang, L.; Li, N.; Hu, Xingwang

doi:10.1159/000125838

Cited by 13 publications

(8 citation statements)

References 50 publications

(28 reference statements)

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“…Only six of these loci were mapped on a microsatellite based genetic map by Huang et al (2008), but each of the six were assigned to different linkage groups (of 13 linkage groups: CAU17 on 1, CAU69 on 11, CAU97 on 4, CAU84 on 9, CAU3 on 2, and CAU14 on 6). The loci were arranged into five multiplexes based on common annealing temperature (T a ) and size ranges (Table 2).…”

Section: Microsatellite Amplification/genotypingmentioning

confidence: 99%

Phylogeographic analysis of nuclear and mtDNA supports subspecies designations in the ostrich (Struthio camelus)

et al. 2010

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We investigated the phylogeography and subspecies classification of the ostrich (Struthio camelus) by assessing patterns of variation in mitochondrial DNA control region (mtDNA-CR) sequence and across fourteen nuclear microsatellite loci. The current consensus taxonomy of S. camelus names five subspecies based on morphology, geographic range, mtDNA restriction fragment length polymorphism and mtDNA-CR sequence analysis: S. c. camelus, S. c. syriacus, S. c. molybdephanes, S. c. massaicus and S. c. australis. We expanded a previous mtDNA dataset from 18 individual mtDNA-CR sequences to 123 sequences, including sequences from all five subspecies. Importantly, these additional sequences included 43 novel sequences of the red-necked ostrich, S. c. camelus, obtained from birds from Niger. Phylogeographic reconstruction of these sequences matches previous results, with three well-supported clades containing S. c. camelus/syriacus, S. c. molybdophanes, and S. c. massaicus/australis, respectively. The 14 microsatellite loci assessed for 119 individuals of four subspecies (all but S. c. syriacus) showed considerable variation, with an average of 13.4 (±2.0) alleles per locus and a mean observed heterozygosity of 55.7 (±5.3)%. These data revealed high levels of variation within most subspecies, and a structure analysis revealed strong separation between each of the four subspecies. The level of divergence across both marker types suggests the consideration of separate species status for S. c. molybdophanes, and perhaps also for S. c. camelus/syriacus. Both the mtDNA-CR and microsatellite analyzes also suggest that there has been no recent hybridization between the subspecies. These findings are of importance for management of the highly endangered red-necked subspecies (S. c. camelus) and may warrant its placement onto the IUCN red list of threatened animals.

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Section: Microsatellite Amplification/genotypingmentioning

confidence: 99%

Phylogeographic analysis of nuclear and mtDNA supports subspecies designations in the ostrich (Struthio camelus)

et al. 2010

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“…The DNA was amplified by targeting 23 microsatellite loci that had already been reported in literature. The microsatellite primer sequences were selected out of the studies by Kimwele et al (1998), Tang et al (2003), Kawka et al (2007) and Huang et al (2008) and are described in Table 1. Microsatellite loci with a high observed heterozygosity, >4 alleles and di-nucleotide repeats were selected for this analysis.…”

Section: Methodsmentioning

confidence: 99%

“…It is therefore necessary to confirm these results using a larger sample size and more microsatellite loci. Microsatellites have also been used to construct a preliminary genetic linkage map of the ostrich (Huang et al, 2008). This map can be of benefit for future identification of chromosomal regions affecting quantitative traits such as growth, reproduction and disease susceptibility.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation within and among three ostrich breeds, estimated by using microsatellite markers

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Cloete

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et al. 2012

SA J. An. Sci.

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________________________________________________________________________________ AbstractGenetic diversity within and among three ostrich populations was investigated to corroborate whether these populations can be classified genetically as three different breeds. The DNA of South African Black (SAB, n = 30), Zimbabwean Blue (ZB, n = 32) and Kenyan Redneck (KR, n = 17) birds was assessed for genetic differences using 19 microsatellite loci. The number of alleles, as well as observed and expected heterozygosity of alleles, was determined. Genetic differentiation was measured using the F-statistic (F ST ) and Nei's genetic distance. Significant differences were observed among the three breeds. The SAB and ZB (F ST = 0.10 and Nei = 0.49) were genetically more similar, whereas the genetic distance between the KR and ZB breeds was the greatest (F ST = 0.13 and Nei = 0.61). The SAB strain exhibited the greatest observed heterozygosity (H o = 0.72) within its population while the ZB and the KR exhibited lower levels of heterozygosity (H o = 0.68). Based on these results, it was suggested that crossbreeding between these breeds would lead to heterosis in commercial ostrich enterprises. ________________________________________________________________________________

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“…Recent interest in ratite farming, especially ostrich and emu, has led to an increasing demand for information about these birds [5, 13, 23], especially the genetics aspects [6, 11, 14, 17–19, 25, 26]. These studies are aimed at determining the genetic structure of these birds e.g., estimation of the genetic variability and analysis of the relationship between individuals belonging to a given populations [28].…”

Section: Introductionmentioning

confidence: 99%

“…It should be emphasized that currently in available literature there is a shortage of data on genetic linkage maps for any ratites. First, a preliminary genetic map of ostrich developed Huang et al [14], analyzing 104 polymorphic microsatellite markers using a two-generation ostrich reference family.…”

Section: Introductionmentioning

confidence: 99%

The use of microsatellite polymorphism in genetic mapping of the ostrich (Struthio camelus)

et al. 2011

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The aim of this study was to determine microsatellite polymorphism in ostriches and using it in creation the genetic map of the ostrich. The polymorphism analysis covered 30 microsatellite markers characteristic of ostrich, for the CAU (China Agricultural University) group. The material consisted of 150 ostriches (Struthio camelus). The 30 microsatellite loci was examined and a total of 343 alleles was identified. The number of alleles at a single locus ranged from 5 at locus CAU78 to 34 at locus CAU85. The values for the observed heterozygosity Ho ranged from 0.467 (locus CAU78) to 0.993 (locus CAU16), whereas for the expected heterozygosity He - from 0.510 (locus CAU78) to 0.953 (locus CAU85). Analyzing the individual loci, the highest PIC value, more than 0.7 was observed for: loci CAU85 (0.932), CAU64 (0.861) and CAU32, 75 (0.852), respectively. It should be noted, that the microsatellite markers used in our study were very polymorphic as evidenced by the large number of detected alleles and high rates of heterozygosity, PIC and PE as well. The analysed microsatellite markers may be used in genetic linkage mapping of ostrich, the construction of a comparative genetic map with other ratites, such as emu and rhea, and population genetics studies or phylogenetic studies of these birds.

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A preliminary microsatellite genetic map of the ostrich (<i>Struthio camelus</i>)

Cited by 13 publications

References 50 publications

Phylogeographic analysis of nuclear and mtDNA supports subspecies designations in the ostrich (Struthio camelus)

Phylogeographic analysis of nuclear and mtDNA supports subspecies designations in the ostrich (Struthio camelus)

Genetic variation within and among three ostrich breeds, estimated by using microsatellite markers

The use of microsatellite polymorphism in genetic mapping of the ostrich (Struthio camelus)

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