A preliminary study of activation of collagenase in carious human dentine matrix

Dayan, Dan; Binderman, Itzhak; Mechanic, Gerald L.

doi:10.1016/0003-9969(83)90126-7

Cited by 91 publications

(67 citation statements)

References 13 publications

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“…Unluckily, there is no clear explanation for this issue. As caries-affected dentin has been reported to exhibit increased collagenolytic activity when compared with sound dentin (16), it could be speculated that CHX application could inhibit the degradation of cariesaffected dentin-bonded interfaces in comparison with sound dentin (25). The higher porosity of caries-affected intertubular dentin may help in gaining the benefit of CHX pretreatment by allowing more penetration of CHX to deeper layers vulnerable to MMPs.…”

Section: Discussionmentioning

confidence: 99%

Chlorhexidine does not increase immediate bond strength of etch-and-rinse adhesive to caries-affected dentin of primary and permanent teeth

et al. 2012

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The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) on immediate bond strength of etch-andrinse adhesive to sound (SD) and caries-affected (CAD) primary dentin compared with permanent dentin. Flat dentin surfaces from 20 primary molars (Pri) and 20 permanent molars (Perm) were assigned to 8 experimental groups (n=5) according to tooth type (Pri or Perm), dentin condition (SD or CAD -pH-cycling for 14 days) and treatment (control -C or 60 s application of 2% CHX solution after acid etching -CHX). The bonding system (Adper Single Bond 2) was applied according to manufacturer's instructions followed by resin composite application (Filtek Z250). After 24 h water storage, specimens with cross-section area of 0.8 mm 2 were prepared for being tested under microtensile test (1 mm/min). Data were submitted to ANOVA and Tukey's post hoc test (α=0.05). Failure mode was evaluated using a stereomicroscope at ×400. Treatment with CHX did not result in higher bond strength values than no pretreatment (C groups), independently of tooth type. Primary teeth and caries-affected dentin showed significantly lower (p<0.05) bond strength means compared with permanent teeth and sound dentin, respectively. Predominance of adhesive/mixed failure was observed for all groups. CHX did not influence the immediate bond strength to sound or caries-affected dentin of primary and permanent teeth.

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Section: Discussionmentioning

confidence: 99%

Chlorhexidine does not increase immediate bond strength of etch-and-rinse adhesive to caries-affected dentin of primary and permanent teeth

et al. 2012

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“…22,44 Accordingly, whether SE Bond was able to activate MMPs to a different degree as compared to normal dentin requires further research. Pretreatment with 2% CHX, similar to that Figure 3.…”

Section: Discussionmentioning

confidence: 99%

Effect of Chlorhexidine Pretreatment on Bond Strength Durability of Caries–affected Dentin Over 2-Year Aging in Artificial Saliva and Under Simulated Intrapulpal Pressure

Mobarak¹

2011

Operative Dentistry

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Pretreatment of caries-affected dentin with 5% chlorhexidine decelerates the bond strength loss over the long term. SUMMARYObjective: To evaluate the influence of 2% and 5% chlorhexidine (CHX) pretreatment on bond durability of a self-etching adhesive to normal (ND) and caries-affected (AD) dentin after 2-years of aging in artificial saliva and under simulated intrapulpal pressure (IPP).Methods: One hundred twenty freshly extracted carious teeth were ground to expose ND and AD. Specimens were distributed into three equal groups (n¼40) according to whether the dentin substrates were pretreated with 2% or 5% CHX or with water (control). Clearfil SE Bond (Kuraray) was applied to both substrates and composite cylinders (0.9 mm diameter and 0.7 mm height) were formed. Pretreatment and bonding were done while the specimens were subjected to 15 mm Hg IPP. After curing, specimens were aged in artificial saliva at 378C and under IPP at 20 mm Hg until being tested after 24 hours or 2 years (n¼20/group). Microshear bond strength was evaluated. Failure modes were determined using a scanning electron microscope (SEM) at 4003 magnification. Data were statistically analyzed using three-way analysis of variance (ANOVA); oneway ANOVA tests, and t-test (p,0.05). Additional specimens (n¼5/group) were prepared to evaluate interfacial silver precipitation.Results: For the 24-hour groups, there were no significant differences among the ND groups and AD groups. For ND aged specimens, the 5% CHX group had the highest value followed by the 2% CHX and control groups, although the difference was statistically insignificant. For AD aged specimens, the 5% CHX group revealed statistically higher bond values compared to the 2% CHX and control groups. Fracture modes were predominately adhesive and mixed. Different interfacial silver depositions were recorded.Conclusions: Two percent or 5% CHX pretreatment has no adverse effect on the 24-hour bonding to ND and AD. Five percent CHX was able to diminish the loss in bonding to AD after 2years of aging in artificial saliva and under simulated IPP.

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“…The first evidence of collagenolytic activity in dentin was reported in the early 1980s both in carious and intact dentin (Dayan et al 1983). More recently, MMPs were identified as being responsible for that activity (Tjäderhane et al 1998), and to date, the presence of gelatinases MMP-2 and -9 ( Fig.…”

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confidence: 99%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

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A preliminary study of activation of collagenase in carious human dentine matrix

Cited by 91 publications

References 13 publications

Chlorhexidine does not increase immediate bond strength of etch-and-rinse adhesive to caries-affected dentin of primary and permanent teeth

Chlorhexidine does not increase immediate bond strength of etch-and-rinse adhesive to caries-affected dentin of primary and permanent teeth

Effect of Chlorhexidine Pretreatment on Bond Strength Durability of Caries–affected Dentin Over 2-Year Aging in Artificial Saliva and Under Simulated Intrapulpal Pressure

Role of Dentin MMPs in Caries Progression and Bond Stability

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