A preliminary study of the effect of low intensity pulsed ultrasound on new bone formation during mandibular distraction osteogenesis in rabbits

Xie, Liangkun; Wangrangsimakul, K.; Suttapreyasri, Srisurang; Cheung, LK; Nuntanaranont, Thongchai

doi:10.1016/j.ijom.2011.03.016

Cited by 20 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…tion during fracture healing and callus distraction quite recently [8][9][10]. Thus, it has been established that LIPUS stimulates bone regeneration in orthopedic field.…”

Section: Open Accessmentioning

confidence: 99%

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Chen¹,

Jia²,

Noritake³

et al. 2013

OJRM

View full text Add to dashboard Cite

Purpose: Low intensity pulsed ultrasound stimulation (LIPUS) has been clinically applied to promote bone fracture healing in the orthopedic field. Thus, it is likely that LIPUS also stimulates bone regeneration in bone defects in the cranial-maxillofacial area. However, this has not been clearly proved. Furthermore, optimal time point and period of the application after the surgery has not been reported. The purpose of the present study was to evaluate the effect of LIPUS on bone regeneration in the rat parietal bone defects especially focusing on time and period of the application. Materials and Methods: Eighteen Wistar rats (14 weeks old) were divided into 6 groups: 5 experimental groups and a control group. Bone defect of 5 mm diameter was prepared on each side of the parietal bone and customized gelatin membranes were placed over the bone defects. LIPUS (160 mW/cm 2 , 15 min/day) was applied to the defect area with an active transducer externally in the experimental groups according to the schedules of the applications: Group 1 (day 6 -12), group 2 (day 13 -19), group 3 (day 20 -26), group 4 (day 6 -19) and group 5 (day 6 -26). All the animals were sacrificed at 28 days. The defects were analyzed with micro CT and then histologically. Results: In Group 1, new bone formation was significantly promoted and the newly-formed bone was thick and matured compared to the one of the control group. In other experimental groups there were tendencies of stimulation of new bone formation; however, they were not statistically significant. Discussion and Conclusion: The present study demonstrated that amount of new bone formation in the bone defect depended on the time and period of LIPUS application. It has been suggested that application of LIPUS at an early healing period, the second week after the surgery, effectively accelerated new bone formation.

show abstract

“…tion during fracture healing and callus distraction quite recently [8][9][10]. Thus, it has been established that LIPUS stimulates bone regeneration in orthopedic field.…”

Section: Open Accessmentioning

confidence: 99%

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Chen¹,

Jia²,

Noritake³

et al. 2013

OJRM

View full text Add to dashboard Cite

show abstract

“…It has been well-established that fracture healing involves four events: inflammation, soft callus formation, hard callus formation and bone remodeling. Pre-clinical work has shown LIPUS to be effective in bone fracture models of small [7–18] and large animals [19–21]. Duatre [7] previously demonstrated that LIPUS accelerated bone fracture union in a rabbit femoral fracture model.…”

Section: Introductionmentioning

confidence: 99%

Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells

Tabuchi

Sugahara

Ikegame

et al. 2013

IJMS

View full text Add to dashboard Cite

Although low-intensity pulsed ultrasound (LIPUS) has been shown to enhance bone fracture healing, the underlying mechanism of LIPUS remains to be fully elucidated. Here, to better understand the molecular mechanism underlying cellular responses to LIPUS, we investigated gene expression profiles in mouse MC3T3-E1 preosteoblast cells exposed to LIPUS using high-density oligonucleotide microarrays and computational gene expression analysis tools. Although treatment of the cells with a single 20-min LIPUS (1.5 MHz, 30 mW/cm2) did not affect the cell growth or alkaline phosphatase activity, the treatment significantly increased the mRNA level of Bglap. Microarray analysis demonstrated that 38 genes were upregulated and 37 genes were downregulated by 1.5-fold or more in the cells at 24-h post-treatment. Ingenuity pathway analysis demonstrated that the gene network U (up) contained many upregulated genes that were mainly associated with bone morphology in the category of biological functions of skeletal and muscular system development and function. Moreover, the biological function of the gene network D (down), which contained downregulated genes, was associated with gene expression, the cell cycle and connective tissue development and function. These results should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells.

show abstract

“…Of which, 31 studies were eliminated after reviewing the abstracts which clearly showed these papers did not meet the criteria. Of the 19 potentially eligible articles retrieved in full text [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28], 2 were excluded as they used high intensity ultrasound (2 W/cm 2 ) [27,28] and 2 studies were published in 2 articles each and were thus considered as one study for the purpose of the research [11,12,23,24], leaving 15 eligible studies included in the systematic review (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…Yes [25] DEXA (BMD) BMD was significantly higher in LIPUS compared to control at early consolidation. BMD in laser group was significantly increased at late consolidation.…”

Section: Discussionmentioning

confidence: 99%

Low Intensity Pulsed Ultrasound for Accelerating Distraction Osteogenesis: A Systematic Review of Experimental Animal Studies

Ginini¹,

Shilo²,

Emodi³

et al. 2018

J. Mol. Clin. Med.

View full text Add to dashboard Cite

The purpose of this report is to review systematically all studies performed regarding the influence of low intensity pulsed ultrasound (LIPUS) on bone regeneration in animals during distraction osteogenesis (DO). Based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist structure, a systematic search using PubMed and EMBASE electronic databases was undertaken utilizing the key words "distraction osteogenesis" and "low intensity ultrasound". Human trials, review articles, case reports and non-English language publications were excluded. Data items were extracted from each eligible study, regarding the study design, risk of bias, results and whether or not LIPUS accelerated bone regeneration. The search identified 40 relevant articles, 15 of which were included for full review. Included studies were characterized by a high risk of bias and considerable variations in study design was observed. However, most studies which reported LIPUS in an intensity of 30-40 mW/cm 2 accelerated bone formation via endochondral ossification, thus shortening the consolidation period when applied during distraction and early consolidation periods. According to the current review, application of LIPUS during DO shows promise in accelerating bone formation and density during DO, that bears no adverse effects, thus shortening the consolidation period. Optimal timing of LIPUS application is during the distraction and early consolidation phases. The preferred intensity should be between 30-40 mW/cm 2. Histological analysis indicates influence via endochondral ossification. Thus, the effect of LIPUS on chondrocytes should be further investigated in order to decipher the exact molecular and cellular influence of LIPUS on enhancement of bone formation. These findings should be used in future clinical protocols and raise potential directions for future research regarding the molecular mechanisms underlying the influence of LIPUS on bone formation.

show abstract

A preliminary study of the effect of low intensity pulsed ultrasound on new bone formation during mandibular distraction osteogenesis in rabbits

Cited by 20 publications

References 23 publications

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells

Low Intensity Pulsed Ultrasound for Accelerating Distraction Osteogenesis: A Systematic Review of Experimental Animal Studies

Contact Info

Product

Resources

About