A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line

Li, Wei; Wang, Yaomei; Song, Yongping; Xu, Lei; Zhao, Junmei; Fang, Baijun

doi:10.3892/ol.2015.2946

Cited by 21 publications

(16 citation statements)

References 29 publications

(41 reference statements)

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“…Our finding indicated that treatment with curcumin-induced apoptosis of the tumor cells by the upregulation in the expression level of the P53 gene in HepG2 cells after administration of curcumin in a time-dependent manner. This result agrees with the previous studies that also reflected the role of curcumin in upregulating P53 protein expression in cancer cells of lymphoma-bearing mice [35], human multiple myeloma cell line RPMI 8226 cells [36] and vascular smooth muscle cell line, A7r5 [37].…”

Section: Discussionsupporting

confidence: 93%

The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma

Khamis¹,

Sharshar²,

Mahmoud³

et al. 2019

JBM

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Curcumin the active component of turmeric is widely used as an anticancer agent for treating many human cancers. This study aimed at the extraction of curcumin from Curcuma Longa and investigates its therapeutic effect as ornithine decarboxylase (ODC) inhibitor in HepG2 cells. The proliferation of HepG2 cells was carried out by using the MTT assay. In addition, cell cycle analysis was evaluated by using the flow-cytometric technique. Our results showed that curcumin has the ability to inhibit the proliferation of HepG2 cells with IC50 of 24.79 μg/ml and induced G2/M cell cycle arrest. Moreover, it caused an elevation in the intracellular concentration of Ca 2+. Moreover, in the curcumin administration the downregulation expression level of ODC and Bcl-2 genes (p ≤ 0.05) was significant found. On the other hand, upregulation in the expression level of P53, Bax, and caspase-3 genes (p ≤ 0.05). This study concluded that curcumin may be considered as a new saving candidate for the future progress of antitumor agents.

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Section: Discussionsupporting

confidence: 93%

The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma

Khamis¹,

Sharshar²,

Mahmoud³

et al. 2019

JBM

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“…It is reported that level of p53 in cells is controlled through many routes but major route is p53-murine double minute 2 (MDM2) pathway through an auto-regulatory feedback loop. Curcumin has shown downregulation of MDM2 leading to upregulation of the expression of p53 and Bax in multiple myeloma cancerous cells (Li et al, 2015). In conclusion, it is revealed that curcumin is effective for activation and restoration of p53 levels especially in the cells with an abnormal p53 expression or function.…”

Section: Epigenetic Modulation By Curcuminmentioning

confidence: 99%

Curcumin as an Alternative Epigenetic Modulator: Mechanism of Action and Potential Effects

et al. 2019

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Curcumin (a polyphenolic compound in turmeric) is famous for its potent anti-inflammatory, anti-oxidant, and anti-cancer properties, and has a great potential to act as an epigenetic modulator. The epigenetic regulatory roles of curcumin include the inhibition of DNA methyltransferases (DNMTs), regulation of histone modifications via the regulation of histone acetyltransferases (HATs) and histone deacetylases (HDACs), regulation of microRNAs (miRNA), action as a DNA binding agent and interaction with transcription factors. These mechanisms are interconnected and play a vital role in tumor progression. The recent research has demonstrated the role of epigenetic inactivation of pivotal genes that regulate human pathologies such as cancers. Epigenetics helps to understand the mechanism of chemoprevention of cancer through different therapeutic agents. In this regard, dietary phytochemicals, such as curcumin, have emerged as a potential source to reverse epigenetic modifications and efficiently regulate the expression of genes and molecular targets that are involved in the promotion of tumorigenesis. The curcumin may also act as an epigenetic regulator in neurological disorders, inflammation, and diabetes. Moreover, curcumin can induce the modifications of histones (acetylation/deacetylation), which are among the most important epigenetic changes responsible for altered expression of genes leading to modulating the risks of cancers. Curcumin is an effective medicinal agent, as it regulates several important molecular signaling pathways that modulate survival, govern anti-oxidative properties like nuclear factor E2-related factor 2 (Nrf2) and inflammation pathways, e.g., nuclear factor kappa B (NF-κB). Curcumin is a potent proteasome inhibitor that increases p-53 level and induces apoptosis through caspase activation. Moreover, the disruption of 26S proteasome activity induced by curcumin through inhibiting DYRK2 in different cancerous cells resulting in the inhibition of cell proliferation opens up a new horizon for using curcumin as a potential preventive and treatment approach in proteasome-linked cancers. This review presents a brief summary of knowledge about the mechanism of epigenetic changes induced by curcumin and the potential effects of curcumin such as anti-oxidant activity, enhancement of wound healing, modulation of angiogenesis and its interaction with inflammatory cytokines. The development of curcumin as a clinical molecule for successful chemo-prevention and alternate therapeutic approach needs further mechanistic insights.

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“…The major features of carcinogenesis have been shown to be inhibited by curcumin 34 . Several mechanisms for anti-cancer activities of curcumin have been proposed, including, induction of apoptosis 34 , p53 stabilization 35 , mTOR 33 , Wnt 36 , Notch 37 , PI3K 38 , signaling inhibition, AMPK activation 39 , cell cycle inhibition 40 , inhibition of oncogenes 41 , inactivation of NF-kB 42 , metastasis inhibition 43 , angiogenesis inhibition 44 , miRNA regulation 45 , DNA damage and repair 46 . However, the effect of curcumin on cancer metabolism, an emerging hallmark of cancer, remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Curcumin decreases Warburg effect in cancer cells by down-regulating pyruvate kinase M2 via mTOR-HIF1α inhibition

Siddiqui

Prakasam

Chattopadhyay

et al. 2018

Sci Rep

120

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Warburg effect is an emerging hallmark of cancer cells with pyruvate kinase M2 (PKM2) as its key regulator. Curcumin is an extensively-studied anti-cancer compound, however, its role in affecting cancer metabolism remains poorly understood. Herein, we show that curcumin inhibits glucose uptake and lactate production (Warburg effect) in a variety of cancer cell lines by down-regulating PKM2 expression, via inhibition of mTOR-HIF1α axis. Stable PKM2 silencing revealed that PKM2 is required for Warburg effect and proliferation of cancer cells. PKM2 over-expression abrogated the effects of curcumin, demonstrating that inhibition of Warburg effect by curcumin is PKM2-mediated. High PKM2 expression correlated strongly with poor overall survival in cancer, suggesting the requirement of PKM2 in cancer progression. The study unravels novel PKM2-mediated inhibitory effect of curcumin on metabolic capacities of cancer cells. To the best of our knowledge, this is the first study linking curcumin with PKM2-driven cancer glycolysis, thus, providing new perspectives into the mechanism of its anticancer activity.

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A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line

Cited by 21 publications

References 29 publications

The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma

The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma

Curcumin as an Alternative Epigenetic Modulator: Mechanism of Action and Potential Effects

Curcumin decreases Warburg effect in cancer cells by down-regulating pyruvate kinase M2 via mTOR-HIF1α inhibition

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