A preliminary study on the stabilization of blood typing antibodies sorbed into paper

Guan, Liyun; Cao, Rong; Tian, Junfei; McLiesh, Heather; Garnier, Gil; Shen, Wei

doi:10.1007/s10570-013-0134-x

Cited by 36 publications

(37 citation statements)

References 26 publications

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“…However, the plasma phase still moves forward, separating from agglutinated RBCs. 25 Most recently, Li et al used calcium chloride solution to aggregate RBCs in rabbit blood samples. However, its application for O Rh negative, a phenotype that has a signicantly high frequency in human population, 12 and weak ABO subgroups 12,23 have not been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Low-cost blood plasma separation method using salt functionalized paper

Nilghaz

Shen

2015

RSC Adv.

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This study describes an extremely low-cost method for separating plasma in a sample of whole human blood on salt functionalized paper by means of osmotic pressure. When a sample of whole blood was introduced onto the salt functionalized paper, plasma dissolves the salt and places the red blood cells (RBCs) in a hypertonic medium. This leads to the generation of osmotic pressure across the cells membrane, and also the crenation of RBCs. The effect of different concentrations of salt on RBC deformation and crenation has been monitored using confocal microscopy. Depending upon the salt concentration, RBCs deform into various shapes under osmotic pressure. At high salt concentration, RBCs turn into deflated thin disks. This increases the RBCs' contact with one another and with fibres in the paper as well. Besides, the counter ion valency charge of the Na + suppresses the thickness of the charged double layer of RBC. Subsequently, aggregation of the deflated RBCs occurs. The aggregates are large enough to be separated chromatographically from the plasma phase of the wicking front.Our results show that 0.5 mL addition of 0.68 M (4% w/v) saline solution (NaCl) can provide sufficient plasma separation on a filter paper for diagnostic applications. A colorimetric blood glucose concentration assay is employed to demonstrate the efficiency of this plasma separation method on paper. The experimental investigation indicates that although the crenation of RBCs forced a small amount of water into plasma, this method is suitable for performing glucose assay in human blood on paper. Our method can enhance bioassays performed on microfluidic paper-based analytical devices (mPADs) by combining the separation and testing of plasma into a single device with no significant additional cost.

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Section: Introductionmentioning

confidence: 99%

Low-cost blood plasma separation method using salt functionalized paper

Nilghaz

Shen

2015

RSC Adv.

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“…Since thermal stability is a very important performance indicator of PADs for sensor applications (Guan et al 2014), both APTMS-and chitosan-modified papers loaded with QDs were aged at different temperatures to test their thermal stability. Thermal stability was evaluated by measuring the red intensities of QDs spots formed in the center of the paper before and after aging.…”

Section: Thermal Stability Of Qds On Modified Papermentioning

confidence: 99%

Surface Modification of Cellulose Paper for Quantum Dot-based Sensing Applications

Guan¹,

Tian²,

Cao³

et al. 2015

BioResources

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Cellulose paper specimens with and without surface modification were compared in order to study their physicochemical compatibility with quantum dots (QDs) for biochemical sensing applications. Silane and chitosan modification methods were applied. The distribution of QDs deposited on untreated paper and papers modified with silane and chitosan were investigated in order to understand the interaction between QDs and fibre. Modified papers were shown to significantly reduce the undesirable redistribution of QDs during paper drying. The retention ability and thermal resistance of QDs to the loss of fluorescence on modified papers were also studied for the purpose of determining the most suitable paper surface modification for developing QD-Paper-based analytical devices (QD-PADs). Furthermore, chitosan-modified paper was used to design QD-PADs to quantify glucose concentration in aqueous samples; the quenching effect of the enzymatic product on the fluorescent emission of QDs was used as the indicator system. The change of fluorescence of QDs was measured by a simple in-house constructed fluorescence imaging system. The detection limit of glucose was 5 mg/dL, which is comparable with other reported paper sensors for detection of glucose.

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“…These properties make cellulose an attractive material for engineering advanced applications such as low-cost, biodegradable, and disposable biodiagnostics (Su et al, 2012; Arcot et al, 2015), point of care analytical devices (Cranston et al, 2010), and membranes (Sukma and Culfaz-Emecen, 2018) for separation (dialysis). Currently, the main biomedical applications of cellulose include biodiagnostics, such as blood typing (Guan et al, 2014; Li et al, 2014; Casals-Terre et al, 2018), and pregnancy tests (Halpern et al, 2008; Koczula and Gallotta, 2016), tissue engineering (Courtenay et al, 2018; Torgbo and Sukyai, 2018; Curvello et al, 2019), scaffolds (Novotna et al, 2013; Modulevsky et al, 2014; Demitri et al, 2016; Courtenay et al, 2017; O'donnell et al, 2018), eye care solutions (Vehige et al, 2003; Luchs, 2010; Nguyen and Latkany, 2011; Racic et al, 2019), coatings (Jimenez et al, 2017; Spera et al, 2017; Sharif Hossain et al, 2018), packaging (Czaja et al, 2007; Sunasee et al, 2016; Shaghaleh et al, 2018), and sensors (Zhu et al, 2014; Ummartyotin and Manuspiya, 2015; Chen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Cellulose Nano-Films as Bio-Interfaces

Raghuwanshi

Garnier

2019

Front. Chem.

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Cellulose, the most abundant polymer on earth, has enormous potential in developing bio-friendly, and sustainable technological products. In particular, cellulose films of nanoscale thickness (1–100 nm) are transparent, smooth (roughness <1 nm), and provide a large surface area interface for biomolecules immobilization and interactions. These attractive film properties create many possibilities for both fundamental studies and applications, especially in the biomedical field. The three liable—OH groups on the monomeric unit of the cellulose chain provide schemes to chemically modify the cellulose interface and engineer its properties. Here, the cellulose thin film serves as a substrate for biomolecules interactions and acts as a support for bio-diagnostics. This review focuses on the challenges and opportunities provided by engineering cellulose thin films for controlling biomolecules interactions. The first part reviews the methods for preparing cellulose thin films. These are by dispersing or dissolving pure cellulose or cellulose derivatives in a solvent to coat a substrate using the spin coating, Langmuir-Blodgett, or Langmuir-Schaefer method. It is shown how different cellulose sources, preparation, and coating methods and substrate surface pre-treatment affect the film thickness, roughness, morphology, crystallinity, swelling in water, and homogeneity. The second part analyses the bio-macromolecules interactions with the cellulose thin film interfaces. Biomolecules, such as antibodies and enzymes, are adsorbed at the cellulose-liquid interface, and analyzed dry and wet. This highlights the effect of film surface morphology, thickness, crystallinity, water intake capacity, and surface pre-treatment on biomolecule adsorption, conformation, coverage, longevity, and activity. Advance characterization of cellulose thin film interface morphology and adsorbed biomolecules interactions are next reviewed. X-ray and neutron scattering/reflectivity combined with atomic force microscopy (AFM), quartz crystal microbalance (QCM), microscopy, and ellipsometer allow visualizing, and quantifying the structural morphology of cellulose-biomolecule interphase and the respective biomolecules conformations, kinetics, and sorption mechanisms. This review provides a novel insight on the advantages and challenges of engineering cellulose thin films for biomedical applications. This is to foster the exploration at the molecular level of the interaction mechanisms between a cellulose interface and adsorbed biomolecules with respect to adsorbed molecules morphology, surface coverage, and quantity. This knowledge is to engineer a novel generation of efficient and functional biomedical devices.

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A preliminary study on the stabilization of blood typing antibodies sorbed into paper

Cited by 36 publications

References 26 publications

Low-cost blood plasma separation method using salt functionalized paper

Low-cost blood plasma separation method using salt functionalized paper

Surface Modification of Cellulose Paper for Quantum Dot-based Sensing Applications

Cellulose Nano-Films as Bio-Interfaces

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