A preliminary technique for electro-ejaculation of agouti (Dasyprocta leporina)

Mollineau, W. M.; Adogwa, Andrew; Garcia, Gary Wayne

doi:10.1016/j.anireprosci.2007.07.017

Cited by 20 publications

(21 citation statements)

References 12 publications

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“…A média de defeitos totais encontrada foi de 31,9 ± 13,5%, menor que a média encontrada por Costa e Paula (2005) que observaram variação de defeitos totais entre 15,5 ± 2,4% e 55,5 ± 16,1% em caititus adultos. Já Mollineau et al (2007), ao avaliarem sêmen de cutias (Dasyprocta leporina), verificaram 35,14 ± 2,7% de anormalidades totais. Segundo Mies Filho (1987), animais silvestres mantidos em cativeiro podem ter elevadas porcentagens de células morfologicamente anormais, se comparadas às espécies domésticas.…”

Section: Características Seminaisunclassified

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

et al. 2010

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Estudos relacionados à obtenção e avaliação de sêmen de Tayassu tajacu são escassos, sendo necessárias pesquisas a respeito. Os objetivos do estudo foram avaliar a biometria testicular de caititus adultos cativos, testar a eficiência da eletroejaculação para obtenção de sêmen e avaliar suas características seminais ao longo do ano. Procedeu-se à eletroejaculação em oito animais adultos e as amostras de sêmen colhidas foram avaliadas quanto às características físicas e morfológicas. Os animais tinham testículo esquerdo com 3,8 ± 0,4 cm X 2,6 ± 0,3 cm e 2,3 ± 0,2 de consistência, e testículo direito com 3,8 ± 0,5 cm X 2,7 ± 0,3 cm e 2,3 ± 0,2 de consistência. A taxa de sucesso nas colheitas foi de 75,21%. O sêmen possuiu: volume 0,81 ± 0,86 mL, concentração 137,44 ± 153 x 106 sptz mL-1, pH 7,92 ± 0,73, motilidade 52,66 ± 28,79%, vigor 2,2 ± 0,8, integridade de membrana plasmática 55,84 ± 28,55%, defeitos maiores 22,87 ± 12,93%, defeitos menores 9,11 ± 5,88% e defeitos totais 31,52 ± 13,81%. Os animais apresentaram simetria testicular, a eletroejaculação se mostrou eficiente para a obtenção de ejaculados em caititus e as flutuações observadas na produção seminal não foram suficientes para caracterizá-los como animais de reprodução sazonal.

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Section: Características Seminaisunclassified

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

et al. 2010

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“…Therefore, obtaining information regarding reproduction of this species will also provide information for their rational production and consequent preservation [11]. In this regard, several studies have been performed involving the reproductive characteristics of agoutis, such as placentation [10], morphology of the ovaries [12] and oviducts [13], semen collection, and semen cryopreservation [14,15].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

Wanderley¹,

Luz²,

Faustino³

et al. 2012

Theriogenology

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].You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol AbstractThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing, all samples were fixed in Carnoy prior to histological analysis. To evaluate ultrastructure, follicles from the control and all cryopreserved treatments were fixed in Karnovsky and processed for transmission electron microscopy. After exposure and freezing, there was a significant decrease in the percentage of morphologically normal preantral follicles in all treatments when compared to the control (92.67 ± 2.79, mean ± SD). However, there were no significant difference when the exposure and freezing procedures were compared using the same cryoprotectant. Moreover, there was no significant difference among cryoprotectants at the time of exposure (DMSO: 64.7 ± 3.8; EG: 70.7 ± 11.2, PROH: 63.3 ± 8.5) or after freezing (DMSO: 60.6 ± 3.6, EG: 64.0 ± 11.9; PROH: 62.0 ± 6.9). However, only follicles frozen with PROH had normal ultrastructure. In conclusion, preantral follicles enclosed in agouti ovarian tissue were successfully cryopreserved using 1.5 M PROH, with satisfactory maintenance of follicle morphology and ultrastructure.

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“…The three Protocols compared depended on the application of incremental stimuli via voltage ranging from 2 to 12 volts and application periods from 2 to 5 seconds [8,10,11]. More variations were observed during the rest periods for these protocols, 2 to 4 seconds for Protocols 1 and 2, and up to 2 minutes between the applications of sequences for Protocol 3.…”

Section: Discussionmentioning

confidence: 99%

“…This line of research also included semen collection by electroejaculation (EE) from Dasyprocta leporina and Dasyprocta azarae in the Republic of Trinidad and Tobago and Brazil, respectively [8][9][10][11]. These procedures were applied while the animals under anaesthesia.…”

Section: Introductionmentioning

confidence: 99%

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Electro Ejaculation of Two Species of Agouti (Dasyprocta spp.) in Brazil and Trinidad and Tobago

Mollineau¹,

Mollineau²

2017

MOJAP

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The meat of Neo-tropical fauna is savoured in parts of the Caribbean, Central and South America. Dasyprocta species is one animal hunted for its meat and this activity may eventually threaten their survival. However, efforts made to domesticate these wild animals lead to the development of farming systems. Reproduction has been a valuable tool in the process of domestication. Therefore, part of this effort included research directed at understanding the reproductive attributes of the male agouti. Electro-ejaculation was used to collect semen from Dasyprocta leporina and Dasyprocta azarae. This mini review compared three electro-ejaculation protocols developed for Dasyprocta species in Brazil and the Republic of Trinidad and Tobago. These procedures were applied after the animals were anaesthetized. Collected ejaculates that contained spermatozoa were expressed as percentages of the total number of ejaculated collected and used as a measure of the effectiveness for the respective three Protocols. The most effective was Protocol 3 (100%). However, this was achieved with the administering of 5 different anaesthetics which may cause some potential damage to the agouti. Conversely, 75% successes were obtained then Xylazine 20mg/ kg (Protocol 1, T4) and Ketamine 20mg/kg and Xylazine 40mg/kg (Protocol 2, T8) were administered, respectively. The ejaculate with the largest concentration of spermatozoa (431±180×10 6 /ml) were collected for Protocol 1, T4. Furthermore, no such specifics were listed for ejaculates collected from Protocol 3. In conclusion, the three Protocols should be reviewed to develop a procedure which utilizes resources efficiently and produces results that are quantitatively and qualitatively desirable.

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A preliminary technique for electro-ejaculation of agouti (Dasyprocta leporina)

Cited by 20 publications

References 12 publications

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

Electro Ejaculation of Two Species of Agouti (Dasyprocta spp.) in Brazil and Trinidad and Tobago

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