A Presumptive Developmental Role for a Sea Urchin Cyclin B Splice Variant

Lozano, Jean-Claude; Schatt, Philippe; Marquès, François; Peaucellier, Gérard; Fort, Philippe; Féral, Jean‐Pierre; Genevière, Anne-Marie; Picard, André

doi:10.1083/jcb.140.2.283

Cited by 30 publications

(17 citation statements)

References 37 publications

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“…These include several CDK regulator cyclins (E, D1, and B) (15)(16)(17), phosphatase CDC25B (18), and p16 INK4a/p19 ARF (19), as well as E2F (20), members of the p53 family (21,22), and Bcl-x (23). Isoforms can behave in dramatically different ways and have different functions.…”

Section: Discussionmentioning

confidence: 99%

Human CDK10 Gene Isoforms

Sergere

Thuret

et al. 2000

Biochemical and Biophysical Research Communications

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Section: Discussionmentioning

confidence: 99%

Human CDK10 Gene Isoforms

Sergere

Thuret

et al. 2000

Biochemical and Biophysical Research Communications

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“…The cell-cycle lengths progressively increase during the development of the embryo for these different kinds of cells (Masuda and Sato, 1984;Yasuda and Schubiger, 1992). The first cells in which the cell cycle slows down are the micromeres in which lengthening of the fifth cell cycle is due to the acquisition of a G2-phase (Lozano et al, 1998). Macromere and mesomere lineages always undergo shorter cell cycles and continue cycling longer than micromere lineage (Masuda and Sato, 1984).…”

Section: Discussionmentioning

confidence: 99%

Embryonic-stage-dependent changes in the level of eIF4E-binding proteins during early development of sea urchin embryos

Salaün

Boulben

Mulner‐Lorillon

et al. 2005

Journal of Cell Science

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The eukaryotic initiation factor 4E (eIF4E)-binding proteins (4E-BPs) inhibit translation initiation by binding eIF4E and preventing recruitment of the translation machinery to mRNA. We have previously shown that fertilization of sea urchin eggs triggers eIF4E–4E-BP complex dissociation and 4E-BP degradation. Here, we show that microinjection of eIF4E-binding motif peptide into unfertilized eggs delays the onset of the first mitosis triggered by fertilization, demonstrating that dissociation of the eIF4E–4E-BP complex is functionally important for the first mitotic division in sea urchin embryos. We also show by gel filtration analyses that eIF4E is present in unfertilized eggs as an 80 kDa molecular mass complex containing 4E-BP and a new 4E-BP of 40 kDa. Fertilization triggers the dissociation of eIF4E from these two 4E-BPs and triggers the rapid recruitment of eIF4E into a high-molecular-mass complex. Release of eIF4E from the two 4E-BPs is correlated with a decrease in the total level of both 4E-BPs following fertilization. Abundance of the two 4E-BPs has been monitored during embryonic development. The level of the two proteins remains very low during the rapid cleavage stage of early development and increases 8 hours after fertilization. These results demonstrate that these two 4E-BPs are down- and upregulated during the embryonic development of sea urchins. Consequently, these data suggest that eIF4E availability to other partners represents an important determinant of the early development of sea urchin embryos.

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“…(A) Northern blot analysis of sea urchin RNAs: The 536-bp fragment isolated by PCR was used as a probe. Lanes contain alternatively 1 g poly A ϩ RNA and 10 g total RNA from unfertilized eggs (UF), 2-cell embryos (2C), and swimming blastulae (Blast) prepared as described (19) Fig. 2.…”

Section: Expression and Localization Of The Sg Cdc2l5 Protein In Tranmentioning

confidence: 99%